Dr. Dirk Albrecht

Senior Scientist

Department for Microbial Physiology and Molecular Biology

Felix-Hausdorff-Straße 8 - 1. floor - room-nr.: 1.59
17489 Greifswald

phone: +49 (0)3834 420 5919
fax:      +49 (0)3834 420 5902
email:   dirkalbr(at)uni-greifswald(dot)de

Curriculum Vitae
since 09/2013 Scientist at the Institute of Microbiology at the University of Greifswald, Department of Microbial Physiology, Prof. Katharina Riedel, main focus mass spectrometry.
04/2002 – 08/2013 Postdoc at the Institute of Microbiology at the University of Greifswald, Department of Microbial Physiology, Prof. Michael Hecker, main focus mass spectrometry
10/2001 - 03/2002 Scientist at the Institute of Organic Chemistry
03/1996 – 10/2001 dissertation and PhD with Professor Dr. A. Hetzheim at the Institute of Organic Chemistry, at the University of Greifswald
01/1995 – 01/1996 Diploma at the Institute of Organic Chemistry at the University of Greifswald
09/1994 – 12/1994 practical training at the research Department Boehringer Ingelheim
09/1990 – 09/1994 Study of Chemistry at the Ernst-Moritz-Arndt-University of Greifswald
Research Interests
  • high throughput spot cutting and spot digestion with the Spot-Handling-Workstation
  • protein/peptide identification with MALDI mass spectrometry
  • gel-free label-based and label-free proteomics with LCMS (Orbitrap XL and MALDI-LCMS)
  • Webmaster
  • Mehdi Eslamizad, Dirk Albrecht, Björn Kuhla. The effect of chronic, mild heat stress on metabolic changes of nutrition  and adaptations in rumen papillae of lactating dairy cows. Journal of Dairy Science Vol. 103 No. 9, 2020. https://doi.org/10.3168/jds.2020-18417.
  • Quintieri L, Fanelli F, Zühlke D, Caputo L, Logrieco AF, Albrecht D and Riedel K. Biofilm and Pathogenesis-Related Proteins in the Foodborne ITEM 17298 With Distinctive Phenotypes During Cold Storage. Front Microbiol. 2020;11:991. PMID:32670211
  • Schultz D, Zühlke D, Bernhardt J, et al. An optimized metaproteomics protocol for a holistic taxonomic and functional characterization of microbial communities from marine particles. Environ Microbiol Rep. 2020 Apr 12. 12842. PMID:32281239
  • Eymann C, Wachlin G, Albrecht D, et al. Exoproteome Analysis of Human Pathogenic Dermatophyte Species and Identification of Immunoreactive Proteins. Proteomics Clin Appl. 2018 Jun 28;:e1800007. PMID: 29952123.
  • Handtke S, Albrecht D, Otto A, Becher D, Hecker M, Voigt B,. The proteomic response of B. pumilus cells to glucose starvation. Proteomics.2017 Nov 30. PMID: 29193752.
  • Weitzel JM, Viergutz T, Albrecht D, Bruckmaier R, Schmicke M, Tuchscherer A, Koch F, Kuhla B. Hepatic thyroid signaling of heat-stressed late pregnant and early lactating cows. J Endocrinol.2017 Aug;234(2):129-141. PMID: 28500083.
  • Handtke S, Albrecht D, Zühlke D, Otto A, Becher D, Schweder T, Riedel K, Hecker M, Voigt B,. Bacillus pumilus KatX2 confers enhanced hydrogen peroxide resistance to a Bacillus subtilis PkatA:katX2 mutant strain. Microb Cell Fact.2017 Apr 26;16(1):72. PMID: 28446175.
  • Eymann C, Lassek C, Wegner U, Bernhardt J, Fritsch OA, Fuchs S, Otto A, Albrecht D, Schiefelbein U, Cernava T, Aschenbrenner I, Berg G, Grube M, Riedel K. Symbiotic interplay of fungi, algae, and bacteria within the lung lichen </i>Lobaria pulmonaria </i> L. Hoffm. as assessed by <i>state-of-the-art</i> metaproteomics. J Proteome Res.2017 Mar 14. PMID: 28290203.
  • Knight AM, Nobili A, van den Bergh T, Genz M, Joosten HJ, Albrecht D, Riedel K, Pavlidis IV, Bornscheuer UT. Bioinformatic analysis of fold-type III PLP-dependent enzymes discovers multimeric racemases. Appl Microbiol Biotechnol.2017 Feb;101(4):1499. PMID: 27787586.
  • Zwicker P, Schultze N, Niehs S, Methling K, Wurster M, Albrecht D, Bernhardt J, Wachlin G, Lalk M, Lindequist U, Haertel B. A proteomic approach for the identification of immunotoxic properties of Tulipalin A. Proteomics.2016 Sep 30. PMID: 27687999.
  • Kaval KG, Hahn B, Tusamda N, Albrecht D, Halbedel S. The PadR-like transcriptional regulator LftR ensures efficient invasion of Listeria monocytogenes into human host cells. Front Microbiol.2015 Jul 28;6:772. PMID: 26284051.
  • Kohler C,, Lourenço RF, Bernhardt J, Albrecht D, Schüler J, Hecker M, Gomes SL. A comprehensive genomic, transcriptomic and proteomic analysis of a hyperosmotic stress sensitive α-proteobacterium. BMC Microbiol.2015 Mar 26;15:71. PMID: 25879753.
  • Voigt B, Albrecht D, Sievers S, Becher D, Bongaerts J, Evers S, Schweder T, Maurer KH, Hecker M. High-resolution proteome maps of Bacillus licheniformis cells growing in minimal medium. Proteomics.2015 Aug;15(15):2629-33. PMID: 25867794.
  • Brack C, Mikolasch A, Schlueter R, Otto A, Becher D, Wegner U, Albrecht D, Riedel K, Schauer F. Antibacterial metabolites and bacteriolytic enzymes produced by Bacillus pumilus during bacteriolysis of Arthrobacter citreus. Mar Biotechnol (NY).2015 Jun;17(3):290-304. PMID: 25678259.
  • Henning AK, Albrecht D, Riedel K, Mettenleiter TC, Karger A. An alternative method for serum protein depletion/enrichment by precipitation at mildly acidic pH values and low ionic strength. Proteomics.2015 Jun;15(11):1935-40. PMID: 25644273.
  • Barbieri G, Voigt B, Albrecht D, Hecker M, Albertini AM, Sonenshein AL, Ferrari E, Belitsky BR. CodY regulates expression of the Bacillus subtilis extracellular proteases Vpr and Mpr. J Bacteriol.2015 Apr;197(8):1423-32. PMID: 25666135.
  • Kierul K, Voigt B, Albrecht D, Chen XH, Carvalhais LC, Borriss R. Influence of root exudates on the extracellular proteome of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42. Microbiology.2015 Jan;161(Pt 1):131-47. PMID: 25355936.
  • Halbedel S, Reiss S, Hahn B, Albrecht D, Mannala GK, Chakraborty T, Hain T, Engelmann S, Flieger A. A systematic proteomic analysis of Listeria monocytogenes house-keeping protein secretion systems. Mol Cell Proteomics.2014 Nov;13(11):3063-81. PMID: 25056936.
  • Palm GJ, Sharma A, Kumari M, Panjikar S, Albrecht D, Jagannadham MV, Hinrichs W. Post-translational modification and extended glycosylation pattern of a plant latex peroxidase of native source characterized by X-ray crystallography. FEBS J.2014 Sep;281(18):4319-33. PMID: 24980207.
  • Wollenhaupt K, Brüssow KP, Albrecht D, Tomek W. The Akt/mTor signaling cascade is modified during placentation in the porcine uterine tissue. Reprod Biol.2013 Sep;13(3):184-94. PMID: 24011189.
  • Kabisch J, Pratzka I, Meyer H, Albrecht D, Lalk M, Ehrenreich A, Schweder T. Metabolic engineering of Bacillus subtilis for growth on overflow metabolites. Microb Cell Fact.2013 Jul 25;12:72. PMID: 23886069.
  • Skovager A, Larsen MH, Castro-Mejia JL, Hecker M, Albrecht D, Gerth U, Arneborg N, Ingmer H. Initial adhesion of Listeria monocytogenes to fine polished stainless steel under flow conditions is determined by prior growth conditions. Int J Food Microbiol.2013 Jul 1;165(1):35-42. PMID: 23685728.
  • Wollenhaupt K, Brüssow KP, Albrecht D, Tomek W. The eIF4E repressor protein 4E-BP2 is merely truncated, despite 4E-BP1 degradation in the porcine uterine tissue during implantation. Mol Reprod Dev.2012 Nov;79(11):767-76. PMID: 22968905.
  • Handtke S, Schroeter R, Jürgen B, Methling K, Schlüter R, Albrecht D, van Hijum SA, Bongaerts J, Maurer KH, Lalk M, Schweder T, Hecker M, Voigt B. Bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress. PLoS One.2014 Jan 20;9(1):e85625. PMID: 24465625.
  • Schroeter R, Hoffmann T, Voigt B, Meyer H, Bleisteiner M, Muntel J, Jürgen B, Albrecht D, Becher D, Lalk M, Evers S, Bongaerts J, Maurer KH, Putzer H, Hecker M, Schweder T, Bremer E. Stress responses of the industrial workhorse Bacillus licheniformis to osmotic challenges. PLoS One.2013 Nov 15;8(11):e80956. PMID: 24348917.
  • Feng J, Michalik S, Varming AN, Andersen JH, Albrecht D, Jelsbak L, Krieger S, Ohlsen K, Hecker M, Gerth U, Ingmer H, Frees D. Trapping and proteomic identification of cellular substrates of the ClpP protease in Staphylococcus aureus. J Proteome Res.2013 Feb 1;12(2):547-58. PMID: 23253041.
  • Wiegand S, Voigt B, Albrecht D, Bongaerts J, Evers S, Hecker M, Daniel R, Liesegang H. Fermentation stage-dependent adaptations of Bacillus licheniformis during enzyme production. Microb Cell Fact.2013 Dec 6;12:120. PMID: 24313996.
  • Fuchs S, Zühlke D, Pané-Farré J, Kusch H, Wolf C, Reiß S, Binh le TN, Albrecht D, Riedel K, Hecker M, Engelmann S. Aureolib – a proteome signature library: towards an understanding of staphylococcus aureus pathophysiology. PLoS One.2013 Aug 13;8(8):e70669. PMID: 23967085.
  • Voigt B, Schroeter R, Jürgen B, Albrecht D, Evers S, Bongaerts J, Maurer KH, Schweder T, Hecker M. The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon. Proteomics.2013 Jul;13(14):2140-61. PMID: 23592518.
  • Moche M, Albrecht D, Maaß S, Hecker M, Westermeier R, Büttner K. The new horizon in 2D electrophoresis: new technology to increase resolution and sensitivity. Electrophoresis.2013 Jun;34(11):1510-8. PMID: 23494680.
  • Zhou X, Szeker K, Janocha B, Böhme T, Albrecht D, Mikhailopulo IA, Neubauer P. Recombinant purine nucleoside phosphorylases from thermophiles: preparation, properties and activity towards purine and pyrimidine nucleosides. FEBS J.2013 Mar;280(6):1475-90. PMID: 23332162.
  • Feng J, Michalik S, Varming AN, Andersen JH, Albrecht D, Jelsbak L, Krieger S, Ohlsen K, Hecker M, Gerth U, Ingmer H, Frees D. Trapping and proteomic identification of cellular substrates of the ClpP protease in Staphylococcus aureus. J Proteome Res.2013 Feb 1;12(2):547-58. PMID: 23253041.
  • Golec P, Karczewska-Golec J, Voigt B, Albrecht D, Schweder T, Hecker M, Wegrzyn G, Łos M. Proteomic profiles and kinetics of development of bacteriophage T4 and its rI and rIII mutants in slowly growing Escherichia coli. J Gen Virol.2013 Apr;94(Pt 4):896-905. PMID: 23239571.
  • Chi BK, Roberts AA, Huyen TT, Bäsell K, Becher D, Albrecht D, Hamilton CJ, Antelmann H. S-bacillithiolation protects conserved and essential proteins against hypochlorite stress in firmicutes bacteria. Antioxid Redox Signal.2013 Apr 10;18(11):1273-95. PMID: 22938038.
  • Reder A, Albrecht D, Gerth U, Hecker M. Cross-talk between the general stress response and sporulation initiation in Bacillus subtilis – the σ(B) promoter of spo0E represents an AND-gate. Environ Microbiol.2012 Oct;14(10):2741-56. PMID: 22524514.
  • Kleiner M, Wentrup C, Lott C, Teeling H, Wetzel S, Young J, Chang YJ, Shah M, VerBerkmoes NC, Zarzycki J, Fuchs G, Markert S, Hempel K, Voigt B, Becher D, Liebeke M, Lalk M, Albrecht D, Hecker M, Schweder T, Dubilier N. Metaproteomics of a gutless marine worm and its symbiotic microbial community reveal unusual pathways for carbon and energy use. Proc Natl Acad Sci U S A.2012 May 8;109(19):E1173-82. PMID: 22517752.
  • Palm GJ, Khanh Chi B, Waack P, Gronau K, Becher D, Albrecht D, Hinrichs W, Read RJ, Antelmann H. Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR. Nucleic Acids Res.2012 May;40(9):4178-92. PMID: 22238377.
  • Thürmer A, Voigt B, Angelov A, Albrecht D, Hecker M, Liebl W. Proteomic analysis of the extremely thermoacidophilic archaeon Picrophilus torridus at pH and temperature values close to its growth limit. Proteomics.2011 Dec;11(23):4559-68. PMID: 22114103.
  • Gardebrecht A, Markert S, Sievert SM, Felbeck H, Thürmer A, Albrecht D, Wollherr A, Kabisch J, Le Bris N, Lehmann R, Daniel R, Liesegang H, Hecker M, Schweder T. Physiological homogeneity among the endosymbionts of Riftia pachyptila and Tevnia jerichonana revealed by proteogenomics. ISME J.2012 Apr;6(4):766-76. PMID: 22011719.
  • Kolata J, Bode LG, Holtfreter S, Steil L, Kusch H, Holtfreter B, Albrecht D, Hecker M, Engelmann S, van Belkum A, Völker U, Bröker BM. Distinctive patterns in the human antibody response to Staphylococcus aureus bacteremia in carriers and non-carriers. Proteomics.2011 Oct;11(19):3914-27. PMID: 21805632.
  • Kuhla B, Nürnberg G, Albrecht D, Görs S, Hammon HM, Metges CC. Involvement of skeletal muscle protein, glycogen, and fat metabolism in the adaptation on early lactation of dairy cows. J Proteome Res.2011 Sep 2;10(9):4252-62. PMID: 21774562.
  • Markert S, Gardebrecht A, Felbeck H, Sievert SM, Klose J, Becher D, Albrecht D, Thürmer A, Daniel R, Kleiner M, Hecker M, Schweder T. Status quo in physiological proteomics of the uncultured Riftia pachyptila endosymbiont. Proteomics.2011 Aug;11(15):3106-17. PMID: 21710568.
  • Schroeter R, Voigt B, Jürgen B, Methling K, Pöther DC, Schäfer H, Albrecht D, Mostertz J, Mäder U, Evers S, Maurer KH, Lalk M, Mascher T, Hecker M, Schweder T. The peroxide stress response of Bacillus licheniformis. Proteomics.2011 Jul;11(14):2851-66. PMID: 21674797.
  • Wolf C, Kusch H, Monecke S, Albrecht D, Holtfreter S, von Eiff C, Petzl W, Rainard P, Bröker BM, Engelmann S. Genomic and proteomic characterization of Staphylococcus aureus mastitis isolates of bovine origin. Proteomics.2011 Jun;11(12):2491-502. PMID: 21595036.
  • Wenzel M, Patra M, Albrecht D, Chen DY, Nicolaou KC, Metzler-Nolte N, Bandow JE. Proteomic signature of fatty acid biosynthesis inhibition available for in vivo mechanism-of-action studies. Antimicrob Agents Chemother.2011 Jun;55(6):2590-6. PMID: 21383089.
  • Wilmes B, Kock H, Glagla S, Albrecht D, Voigt B, Markert S, Gardebrecht A, Bode R, Danchin A, Feller G, Hecker M, Schweder T. Cytoplasmic and periplasmic proteomic signatures of exponentially growing cells of the psychrophilic bacterium Pseudoalteromonas haloplanktis TAC125. Appl Environ Microbiol.2011 Feb;77(4):1276-83. PMID: 21183643.
  • Kuhla B, Albrecht D, Bruckmaier R, Viergutz T, Nürnberg G, Metges CC. Proteome and radioimmunoassay analyses of pituitary hormones and proteins in response to feed restriction of dairy cows. Proteomics.2010 Dec;10(24):4491-500. PMID: 21136601.
  • Wollenhaupt K, Reinke K, Brüssow KP, Albrecht D, Tiemann U, Seyfert HM, Tomek W. Natural occurrence and physiological role of a truncated eIF4E in the porcine endometrium during implantation. Biochem J.2010 Dec 1;432(2):353-63. PMID: 20854261.
  • Chi BK, Albrecht D, Gronau K, Becher D, Hecker M, Antelmann H. The redox-sensing regulator YodB senses quinones and diamide via a thiol-disulfide switch in Bacillus subtilis. Proteomics.2010 Sep;10(17):3155-64. PMID: 20652907.
  • Chi BK, Kobayashi K, Albrecht D, Hecker M, Antelmann H. The paralogous MarR/DUF24-family repressors YodB and CatR control expression of the catechol dioxygenase CatE in Bacillus subtilis. J Bacteriol.2010 Sep;192(18):4571-81. PMID: 20639328.
  • Kuhla B, Kucia M, Görs S, Albrecht D, Langhammer M, Kuhla S, Metges CC. Effect of a high-protein diet on food intake and liver metabolism during pregnancy, lactation and after weaning in mice. Proteomics.2010 Jul;10(14):2573-88. PMID: 20422639.
  • Eberhardt C, Engelmann S, Kusch H, Albrecht D, Hecker M, Autenrieth IB, Kempf VA. Proteomic analysis of the bacterial pathogen Bartonella henselae and identification of immunogenic proteins for serodiagnosis. Proteomics.2009 Apr;9(7):1967-81. PMID: 19333998.
  • Kuhla B, Albrecht D, Kuhla S, Metges CC. Proteome analysis of fatty liver in feed-deprived dairy cows reveals interaction of fuel sensing, calcium, fatty acid, and glycogen metabolism. Physiol Genomics.2009 Apr 10;37(2):88-98. PMID: 19240300.
  • Schönhusen U, Kuhla S, Rudolph PE, Zitnan R, Albrecht D, Huber K, Voigt J, Flöter A, Hammon HM, Metges CC. Alterations in the jejunum of young goats caused by feeding soy protein-based diets. J Anim Physiol Anim Nutr (Berl).2010 Feb 1;94(1):1-14. PMID: 19138348.
  • Voigt B, Antelmann H, Albrecht D, Ehrenreich A, Maurer KH, Evers S, Gottschalk G, van Dijl JM, Schweder T, Hecker M. Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol.2009;16(1-2):53-68. PMID: 18957862.
  • Liebeke M, Pöther DC, van Duy N, Albrecht D, Becher D, Hochgräfe F, Lalk M, Hecker M, Antelmann H. Depletion of thiol-containing proteins in response to quinones in Bacillus subtilis. Mol Microbiol.2008 Sep;69(6):1513-29. PMID: 18673455.
  • Wolf C, Hochgräfe F, Kusch H, Albrecht D, Hecker M, Engelmann S. Proteomic analysis of antioxidant strategies of Staphylococcus aureus: diverse responses to different oxidants. Proteomics.2008 Aug;8(15):3139-53. PMID: 18604844.
  • Hieu CX, Voigt B, Albrecht D, Becher D, Lombardot T, Glöckner FO, Amann R, Hecker M, Schweder T. Detailed proteome analysis of growing cells of the planctomycete Rhodopirellula baltica SH1T. Proteomics.2008 Apr;8(8):1608-23. PMID: 18340632.