Prof. Dr. Dörte Becher

Executive Vice Director

Microbial Proteomics

F.-L.-Jahnstr. 15 - 1.floor QB right - room-nr.: 106
17489 Greifswald

phone: +49 (0)3834 86 4230
fax:      +49 (0)3834 86 4202
email:  dbecher(at)uni-greifswald(dot)de

opening hours: only by appointment

Curriculum Vitae
since 10/2014 Managing Vice Director of the Institute of Microbiology, Ernst-Moritz-Arndt University Greifswald
since 06/2013 Professor for Microbial Proteomics at the Institute for Microbiology, Ernst-Moritz-Arndt University Greifswald
01/2012 Habilitation in Microbiology, Topic “Novel qualitative and quantitative approaches for global proteome analysis in gram positive bacteria”
2003 – 2013 Head of the Group “Microbial Proteomics and Mass Spectrometry” at the Institute for Microbiology, Ernst-Moritz-Arndt University Greifswald
1999 – 2013 Scientific Assistant, Institute for Microbiology, in the group of Prof. M. Hecker, Ernst-Moritz-Arndt University Greifswald
1993 – 1998 Ph.D. (Dr. rer. nat., Microbiology) Ernst-Moritz-Arndt University Greifswald, Institute of Microbiology, in the group of Prof. F. Schauer
1992 University of Aberdeen, ERASMUS Students Exchange Program
1987 – 1992 Study of Chemistry, Diploma, Ernst-Moritz-Arndt University Greifswald
Further Activities
since 2011 Secretary-Treasurer of the Bacteriology and Applied Microbiology Division (BAM) of the International Union of Microbiological Societies (IUMS)
Research Interests
  • Development, establishment and optimization of mass spectrometry based proteomics methods with a focus on global characterization of microbial proteomes in a qualitative and quantitative manner
  • Application of newly established methods for the global analysis of protein expression profiles as well as the characterization of single proteins, post translational modified proteins or protein complexes
  • Absolute protein quantification with targeted as well as global methods for determination of proteins on the level of copy number per cells especially in the context of systems biology
  • Elaboration and application of proteomics methods with particular consideration of technologically/industrial relevant questions, infection related conditions or meta-proteomics aspects.

 

 

Selected Funded Projects

Graduate College 1870 “Bacterial Respiratory Infections – Common and Specific Mechanisms of Pathogen Adaption and Immune Response”.

  • Project A1 “Proteome stress signatures and metabolome analysis of Burkholderia pseudomallei and Streptococcus pneumoniae under infection-related in vitro conditions”
  • Project A2 “Proteome and transcriptome signatures of extracellular and intracellular Burkholderia pseudomallei and Streptococcus pneumonia” funded by DFG 2014 -2018

DFG CRC-TRR34 “Pathophysiology of Staphylococci in the Post-Genomic Era”

  • Project Z2 “Staphyloccoccus aureus proteome” funded by DFG 2014 – 2018

CDiff Clostridium difficile – From the proteome inventory to a comprehensive understanding of pathophysiology” financed by VW Vorab & Federal State of Mecklenburg-Vorpommern 2014 – 2016

EUGENPATH “Eukaryotic genes in vacuolar pathogens and symbionts” funded by ERA-Infect program of the European Community 2014 – 2017

ITN Proteinfactory “Engineering of new-generation protein secretion systems” funded by Horizon 2020 – Research and Innovation Framework Program of the European Community 2014 – 2018

European Regional Development Fund (ERDF) 

EASY-nLC 1200 Nano-HPLC

With the support from the European Regional Development Fund (ERDF) it was possible to acquire a nano- HPLC System for the mass spectrometric analysis of complex peptide samples generated in proteomics approaches. The LC- System will be used within the frame of extramurally funded research projects of applied sciences as well as in direct cooperations with industrial partners.

Publication list
2016
  • Ponnudurai R, Kleiner M, Sayavedra L, Petersen JM, Moche M, Otto A, Becher D, Takeuchi T, Satoh N, Dubilier N, Schweder T, Markert S,. Metabolic and physiological interdependencies in the Bathymodiolus azoricus symbiosis. ISME J.2016 Nov 1. PMID: 27801908.

  • König S, Gros O, Heiden SE, Hinzke T, Thürmer A, Poehlein A, Meyer S, Vatin M, Mbéguié-A-Mbéguié D, Tocny J, Ponnudurai R, Daniel R, Becher D, Schweder T, Markert S,. Nitrogen fixation in a chemoautotrophic lucinid symbiosis. Nat Microbiol.2016 Oct 24;2:16193. PMID: 27775698.

  • Stobernack T, Glasner C, Junker S, Gabarrini G, de Smit M, de Jong A, Otto A, Becher D, van Winkelhoff AJ, van Dijl JM. The Extracellular Proteome and Citrullinome of the Oral Pathogen Porphyromonas gingivalis. J Proteome Res.2016 Oct 7. PMID: 27712078.

  • Lappann M, Otto A, Brauer M, Becher D, Vogel U, Johswich K. Impact of moderate temperature changes on Neisseria meningitidis adhesive phenotypes and proteome. Infect Immun.2016 Sep 26. PMID: 27672084.

  • Smirnov A, Förstner KU, Holmqvist E, Otto A, Günster R, Becher D, Reinhardt R, Vogel J. Grad-seq guides the discovery of ProQ as a major small RNA-binding protein. Proc Natl Acad Sci U S A.2016 Oct 11;113(41):11591-11596. PMID: 27671629.

  • Otto A, Biran D, Sura T, Becher D, Ron EZ. Proteomics of septicemic Escherichia coli. Proteomics Clin Appl.2016 Oct;10(9-10):1020-1024. PMID: 27604157.

  • Otto A, Maaß S, Lassek C, Becher D, Hecker M, Riedel K, Sievers S. The protein inventory of Clostridium difficile grown in complex and minimal medium. Proteomics Clin Appl.2016 Oct;10(9-10):1068-1072. PMID: 27511832.

  • Zühlke D, Dörries K, Bernhardt J, Maaß S, Muntel J, Liebscher V, Pané-Farré J, Riedel K, Lalk M, Völker U, Engelmann S, Becher D, Fuchs S, Hecker M. Costs of life – Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis. Sci Rep.2016 Jun 27;6:28172. PMID: 27344979.
  • López-Mondéjar R, Zühlke D, Větrovský T, Becher D, Riedel K, Baldrian P. Decoding the complete arsenal for cellulose and hemicellulose deconstruction in the highly efficient cellulose decomposer Paenibacillus O199. Biotechnol Biofuels. 2016. 9:104. PMID: 27186238

  • Taha MK, Claus H, Lappann M, Veyrier FJ, Otto A, Becher D, Deghmane AE, Frosch M, Hellenbrand W, Hong E, Parent du Châtelet I, Prior K, Harmsen D, Vogel U. Evolutionary Events Associated with an Outbreak of Meningococcal Disease in Men Who Have Sex with Men. PLoS One. 2016. 11(5):e0154047. PMID: 27167067

  • López-Mondéjar R, Zühlke D, Becher D, Riedel K, Baldrian P. Cellulose and hemicellulose decomposition by forest soil bacteria proceeds by the action of structurally variable enzymatic systems. Sci Rep. 2016. 29;6:25279. PMID: 27125755

  • Herweg JA, Pons V, Becher D, Hecker M, Krohne G, Barbier J, Berger H, Rudel T, Mehlitz A. Proteomic analysis of the Simkania-containing vacuole: the central role of retrograde transport. Mol Microbiol. 2016. 99(1):151-71. PMID: 26374382

  • Aurass P, Gerlach T, Becher D, Voigt B, Karste S, Bernhardt J, Riedel K, Hecker M, Flieger A. Life stage-specific proteomes of Legionella pneumophila reveal a highly differential abundance of virulence-associated Dot/Icm effectors. Mol Cell Proteomics. 2016. 15(1):177-200. PMID: 26545400

  • Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Pühler A. Comparative proteome analysis of Actinoplanes sp. SE50/110 grown with maltose or glucose shows minor differences for acarbose biosynthesis proteins but major differences for saccharide transporters. J Proteomics. 2016. 131:140-8. PMID: 26597626

  • Dietz S, Lassek C, Mack SL, Ritzmann M, Stadler J, Becher D, Hoelzle K, Riedel K, Hoelzle LE. Updating the proteome of the uncultivable hemotrophic Mycoplasma suis in experimentally infected pigs. Proteomics. 2016. 16(4):609-13. PMID: 26678042

  • Maaß S, Becher D. Methods and applications of absolute protein quantification in Microbial Systems. J Proteomics. 2016. 136:222-33. PMID: 26825536

  • Bonn F, Pané-Farré J, Schlüter R, Schaffer M, Fuchs S, Bernhardt J, Riedel K, Otto A, Völker U, Dijl JM, Hecker M, Mäder U, Becher D. Global analysis of the impact of linezolid onto virulence factor production in S. aureus USA300. Int J Med Microbiol. 2016. 306(3):131-40. PMID: 26996810
2015

Goelzer A, Muntel J, Chubukov V, Jules M, Prestel E, Nölker R, Mariadassou M, Aymerich S, Hecker M, Noirot P, Becher D, Fromion V. Quantitative prediction of genome-wide resource allocation in bacteria. Metab Eng. 2015; 32:232-43. PMID: 26498510

Sayavedra L, Kleiner M, Ponnudurai R, Wetzel S, Pelletier E, Barbe V, Satoh N, Shoguchi E, Fink D, Breusing C, Reusch TB, Rosenstiel P, Schilhabel MB, Becher D, Schweder T, Markert S, Dubilier N, Petersen JM. Abundant toxin-related genes in the genomes of beneficial symbionts from deep-sea hydrothermal vent mussels. Elife. 2015; 4. pii: e07966. PMID: 26371554

Xing P, Hahnke RL, Unfried F, Markert S, Huang S, Barbeyron T, Harder J, Becher D, Schweder T, Glöckner FO, Amann RI, Teeling H. Niches of two polysaccharide-degrading Polaribacter isolates from the North Sea during a spring diatom bloom. ISME J. 2015; 9(6):1410-22. PMID: 25478683

Singhal A, Arora G, Virmani R, Kundu P, Khanna T, Sajid A, Misra R, Joshi J, Yadav V, Samanta S, Saini N, Pandey AK, Visweswariah SS, Hentschker C, Becher D, Gerth U, Singh Y. Systematic analysis of mycobacterial acylation reveals first example of acylation-mediated regulation of enzyme activity of a bacterial phosphatase. J Biol Chem. 2015; 290(43):26218-34. PMID: 26350458

Heazlewood JL, Schrimpf SP, Becher D, Riedel K, Tholey A, Bendixen E. Multi-Organism Proteomes (iMOP): Advancing our understanding of human biology. Proteomics. 2015;15(17):2885-94. PMID: 26331910

Theilacker C, Diederich AK, Otto A, Sava IG, Wobser D, Bao Y, Hese K, Broszat M, Henneke P, Becher D, Huebner J. Enterococcus faecalis glycolipids modulate lipoprotein-content of the bacterial cell membrane and host immune response. PLoS One. 2015;10(7):e0132949. PMID: 26172831

Neef J, Milder FJ, Koedijk DG, Klaassens M, Heezius EC, van Strijp JA, Otto A, Becher D, van Dijl JM, Buist G. Versatile vector suite for the extracytoplasmic production and purification of heterologous His-tagged proteins in Lactococcus lactis. Appl Microbiol Biotechnol. 2015; 99(21):9037-48. PMID: 26160391

Moche M, Schlüter R, Bernhardt J, Plate K, Riedel K, Hecker M, Becher D. Time-resolved analysis of cytosolic and surface-associated proteins of Staphylococcus aureus HG001 under planktonic and biofilm conditions. J Proteome Res. 2015;14(9):3804-22. PMID: 26152824

Herweg JA, Hansmeier N, Otto A, Geffken AC, Subbarayal P, Prusty BK, Becher D, Hensel M, Schaible UE, Rudel T, Hilbi H. Purification and proteomics of pathogen-modified vacuoles and membranes. Front Cell Infect Microbiol. 2015; 5:48. Review. PMID: 26082896

Kern B, Jain U, Utsch C, Otto A, Busch B, Jiménez-Soto L, Becher D, Haas R. Characterization of Helicobacter pylori VacA-containing vacuoles (VCVs), VacA intracellular trafficking and interference with calcium signalling in T lymphocytes. Cell Microbiol. 2015. 17(12):1811-32. PMID: 26078003

Stopnisek N, Zühlke D, Carlier A, Barberán A, Fierer N, Becher D, Riedel K, Eberl L and Weisskopf L. Molecular mechanisms underlying the close association between soil Burkholderia and fungi. ISME J. 2015. 10(1):253-64. PMID: 25989372

Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Walter F, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Pühler A. Comprehensive proteome analysis of Actinoplanes sp. SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster. J Proteomics. 2015. 125:1-16. PMID: 25896738

Prasse D, Thomsen J, De Santis R, Muntel J, Becher D, Schmitz RA. First description of small proteins encoded by spRNAs in Methanosarcina mazei strain Gö1. Biochimie. 2015; 117:138-48 PMID: 25890157

Voigt B, Albrecht D, Sievers S, Becher D, Bongaerts J, Evers S, Schweder T, Maurer KH, Hecker M. High resolution proteome maps of Bacillus licheniformis cells growing in minimal medium. Proteomics. 2015;15(15):2629-33. PMID: 25867794

Brack C, Mikolasch A, Schlueter R, Otto A, Becher D, Wegner U, Albrecht D, Riedel K, Schauer F. Antibacterial metabolites and bacteriolytic enzymes produced by Bacillus pumilus during bacteriolysis of Arthrobacter citreus. Mar Biotechnol (NY). 2015; 17(3):290-304. PMID: 25678259

Lassek C, Burghartz M, Chaves-Moreno D, Otto A, Hentschker C, Fuchs S, Bernhardt J, Jauregui R, Neubauer R, Becher D, Pieper DH, Jahn M, Jahn D, Riedel K. A Metaproteomics approach to elucidate host and pathogen protein expression during catheter-associated urinary tract infections (CAUTIs). Mol Cell Proteomics. 2015; 14(4):989-1008. PMID: 25673765

Salzberg LI, Botella E, Hokamp K, Antelmann H, Maaß S, Becher D, Noone D, Devine KM. Genome-Wide Analysis of Phosphorylated PhoP Binding to Chromosomal DNA Reveals Several Novel Features of the PhoPR-Mediated Phosphate Limitation Response in Bacillus subtilis. J Bacteriol. 2015; 197(8):1492-506. PMID: 25666134

AbouElfetouh A, Kuhn ML, Hu LI, Scholle MD, Sorensen DJ, Sahu AK, Becher D, Antelmann H, Mrksich M, Anderson WF, Gibson BW, Schilling B, Wolfe AJ. The E. coli sirtuin CobB shows no preference for enzymatic and nonenzymatic lysine acetylation substrate sites. Microbiologyopen. 2015;4(1):66-83. PMID: 25417765

2014

Handtke S, Volland S, Methling K, Albrecht D, Becher D, Nehls J, Bongaerts J, Maurer KH, Lalk M, Liesegang H, Voigt B, Daniel R, Hecker M. Cell physiology of the biotechnological relevant bacterium Bacillus pumilus-An omics-based approach. J Biotechnol. 2014;192 Pt A:204-14. PMID: 25281541

Kohlmann Y, Pohlmann A, Schwartz E, Zühlke D, Otto A, Albrecht D, Grimmler C, Ehrenreich A, Voigt B, Becher D, Hecker M, Friedrich B, Cramm R. Coping with Anoxia: A Comprehensive Proteomic and Transcriptomic Survey of Denitrification. J Proteome Res. 2014; 13(10):4325-38. PMID: 25198380

Maaβ S, Wachlin G, Bernhardt J, Eymann C, Fromion V, Riedel K, Becher D, Hecker M. Highly Precise Quantification of Protein Molecules per Cell During Stress and Starvation Responses in Bacillus subtilis. Mol Cell Proteomics. 2014;13(9):2260-76. PMID: 24878497.

Huja S, Oren Y, Biran D, Meyer S, Dobrindt U, Bernhard J, Becher D, Hecker M, Sorek R, Ron EZ. Fur is the master regulator of the extraintestinal pathogenic Escherichia coli response to serum. MBio. 2014;5(4). pii: e01460-14. PMID: 25118243.

Bonn F, Bartel J, Büttner K, Hecker M, Otto A, Becher D. Picking vanished proteins from the void: how to collect and ship/share extremely dilute proteins in a reproducible and highly efficient manner. Anal Chem. 2014;86(15):7421-7. PMID: 24987932.

Grube M, Cernava T, Soh J, Fuchs S, Aschenbrenner I, Lassek C, Wegner U, Becher D, Riedel K, Sensen CW, Berg G. Exploring functional contexts of symbiotic sustain within lichen-associated bacteria by comparative omics. ISME J. 2014. doi: 10.1038/ismej.2014.138. PMID: 25072413.

Gaballa A, Chi BK, Roberts AA, Becher D, Hamilton CJ, Antelmann H, Helmann JD. Redox regulation in Bacillus subtilis: The bacilliredoxins BrxA(YphP) and BrxB(YqiW) function in de-bacillithiolation of S-bacillithiolated OhrR and MetE. Antioxid Redox Signal. 2014;21(3):357-67. PMID: 24313874.

Hoffmann C, Finsel I, Otto A, Pfaffinger G, Rothmeier E, Hecker M, Becher D, Hilbi H. Functional analysis of novel Rab GTPases identified in the proteome of purified Legionella-containing vacuoles from macrophages. Cell Microbiol. 2014;16(7):1034-52. PMID: 24373249.

Kabisch A, Otto A, König S, Becher D, Albrecht D, Schüler M, Teeling H, Amann RI, Schweder T. Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes ‘Gramella forsetii‘ KT0803. ISME J. 2014;8(7):1492-502. PubMed PMID: 24522261.

Petasch J, Disch EM, Markert S, Becher D, Schweder T, Hüttel B, Reinhardt R, Harder J. The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen. BMC Microbiol. 2014;14:164. PMID: 24952578.

Wenzel M, Chiriac AI, Otto A, Zweytick D, May C, Schumacher C, Gust R, Albada HB, Penkova M, Krämer U, Erdmann R, Metzler-Nolte N, Straus SK, Bremer E, Becher D, Brötz-Oesterhelt H, Sahl HG, Bandow JE. Small cationic antimicrobial peptides delocalize peripheral membrane proteins. Proc Natl Acad Sci U S A. 2014;111(14):E1409-18. PMID: 24706874.

Muntel J, Fromion V, Goelzer A, Maaβ S, Mäder U, Büttner K, Hecker M, Becher D. Comprehensive absolute quantification of the cytosolic proteome of Bacillus subtilis by data independent, parallel fragmentation in liquid chromatography/mass spectrometry (LC/MS(E)). Mol Cell Proteomics. 2014;13(4):1008-19. PMID: 24696501.

Bäsell K, Otto A, Junker S, Zühlke D, Rappen GM, Schmidt S, Hentschker C, Macek B, Ohlsen K, Hecker M, Becher D. The phosphoproteome and its physiological dynamics in Staphylococcus aureus. Int J Med Microbiol. 2014;304(2):121-32. PMID: 24457182.

Otto A, van Dijl JM, Hecker M, Becher D. The Staphylococcus aureus proteome. Int J Med Microbiol. 2014;304(2):110-20. Review. PMID: 24439828.

Otto A, Becher D, Schmidt F. Quantitative proteomics in the field of microbiology. Proteomics. 2014;14(4-5):547-65. PMID: 24376008.

Pribyl T, Moche M, Dreisbach A, Bijlsma JJ, Saleh M, Abdullah MR, Hecker M, van Dijl JM, Becher D, Hammerschmidt S. Influence of impaired lipoprotein biogenesis on surface and exoproteome of Streptococcus pneumoniae. J Proteome Res. 2014;13(2):650-67. PMID: 24387739.

Chi BK, Busche T, Van Laer K, Bäsell K, Becher D, Clermont L, Seibold GM, Persicke M, Kalinowski J, Messens J, Antelmann H. Protein S-mycothiolation functions as redox-switch and thiol protection mechanism in Corynebacterium glutamicum under hypochlorite stress. Antioxid Redox Signal. 2014;20(4):589-605. PMID: 23886307.

Noone D, Salzberg LI, Botella E, Bäsell K, Becher D, Antelmann H, Devine KM. A highly unstable transcript makes CwlO D,L-endopeptidase expression responsive to growth conditions in Bacillus subtilis. J Bacteriol. 2014;196(2):237-47. PMID: 24163346.

Miranda HV, Antelmann H, Hepowit N, Chavarria NE, Krause DJ, Pritz JR, Bäsell K, Becher D, Humbard MA, Brocchieri L, Maupin-Furlow JA. Archaeal ubiquitin-like SAMP3 is isopeptide-linked to proteins via a UbaA-dependent mechanism. Mol Cell Proteomics. 2014;13(1):220-39. PMID: 24097257.

2013

Hessling B, Bonn F, Otto A, Herbst FA, Rappen GM, Bernhardt J, Hecker M, Becher D. Global proteome analysis of vancomycin stress in Staphylococcus aureus. Int J Med Microbiol. 2013;303(8):624-34. PMID: 24161710.

Greiner R, Pálinkás Z, Bäsell K, Becher D, Antelmann H, Nagy P, Dick TP. Polysulfides link H2S to protein thiol oxidation. Antioxid Redox Signal. 2013;19(15):1749-65. PMID: 23646934.

Lappann M, Otto A, Becher D, Vogel U. Comparative proteome analysis of spontaneous outer membrane vesicles and purified outer membranes of Neisseria meningitidis. J Bacteriol. 2013;195(19):4425-35. PMID: 23893116.

Hessling B, Büttner K, Hecker M, Becher D. Global relative quantification with liquid chromatography-matrix-assisted laser desorption ionization time-of-flight (LC-MALDI-TOF)–cross-validation with LTQ-Orbitrap proves reliability and reveals complementary ionization preferences. Mol Cell Proteomics. 2013;12(10):2911-20. PMID: 23788530.

Becher D, Bernhardt J, Fuchs S, Riedel K. Metaproteomics to unravel major microbial players in leaf litter and soil environments: challenges and perspectives. Proteomics. 2013;13(18-19):2895-909. Review. PMID: 23894095.

Krishnappa L, Dreisbach A, Otto A, Goosens VJ, Cranenburgh RM, Harwood CR, Becher D, van Dijl JM. Extracytoplasmic proteases determining the cleavage and release of secreted proteins, lipoproteins, and membrane proteins in Bacillus subtilis. J Proteome Res. 2013;12(9):4101-10. PMID: 23937099.

Hu LI, Chi BK, Kuhn ML, Filippova EV, Walker-Peddakotla AJ, Bäsell K, Becher D, Anderson WF, Antelmann H, Wolfe AJ. Acetylation of the response regulator RcsB controls transcription from a small RNA promoter. J Bacteriol. 2013;195(18):4174-86. PMID: 23852870.

Musa YR, Bäsell K, Schatschneider S, Vorhölter FJ, Becher D, Niehaus K. Dynamic protein phosphorylation during the growth of Xanthomonas campestris pv. campestris B100 revealed by a gel-based proteomics approach. J Biotechnol. 2013;167(2):111-22. PMID: 23792782.

Chi BK, Roberts AA, Huyen TT, Bäsell K, Becher D, Albrecht D, Hamilton CJ, Antelmann H. S-bacillithiolation protects conserved and essential proteins against hypochlorite stress in firmicutes bacteria. Antioxid Redox Signal. 2013;18(11):1273-95. PMID: 22938038.

Tsompanidou E, Denham EL, Becher D, de Jong A, Buist G, van Oosten M, Manson WL, Back JW, van Dijl JM, Dreisbach A. Distinct roles of phenol-soluble modulins in spreading of Staphylococcus aureus on wet surfaces. Appl Environ Microbiol. 2013;79(3):886-95. PMID: 23183971.

Goosens VJ, Otto A, Glasner C, Monteferrante CC, van der Ploeg R, Hecker M, Becher D, van Dijl JM. Novel twin-arginine translocation pathway-dependent phenotypes of Bacillus subtilis unveiled by quantitative proteomics. J Proteome Res. 2013;12(2):796-807. PMID: 23256564.

Schroeter R, Hoffmann T, Voigt B, Meyer H, Bleisteiner M, Muntel J, Jürgen B, Albrecht D, Becher D, Lalk M, Evers S, Bongaerts J, Maurer KH, Putzer H, Hecker M, Schweder T, Bremer E. Stress responses of the industrial workhorse Bacillus licheniformis to osmotic challenges. PLoS One. 2013;8(11):e80956. PMID: 24348917.

Celik H, Blouzard JC, Voigt B, Becher D, Trotter V, Fierobe HP, Tardif C, Pagès S, de Philip P. A two-component system (XydS/R) controls the expression of genes encoding CBM6-containing proteins in response to straw in Clostridium cellulolyticum. PLoS One. 2013;8(2):e56063. PMID: 23418511.

2012

Sibbald MJ, Yang XM, Tsompanidou E, Qu D, Hecker M, Becher D, Buist G, van Dijl JM. Partially overlapping substrate specificities of staphylococcal group A sortases. Proteomics. 2012;12(19-20):3049-62. PMID: 22930668.

Lima BP, Thanh Huyen TT, Bäsell K, Becher D, Antelmann H, Wolfe AJ. Inhibition of acetyl phosphate-dependent transcription by an acetylatable lysine on RNA polymerase. J Biol Chem. 2012;287(38):32147-60. PMID: 22829598.

Michalik S, Bernhardt J, Otto A, Moche M, Becher D, Meyer H, Lalk M, Schurmann C, Schlüter R, Kock H, Gerth U, Hecker M. Life and death of proteins: a case study of glucose-starved Staphylococcus aureus. Mol Cell Proteomics. 2012;11(9):558-70. PMID: 22556279.

Otto A, Bernhardt J, Hecker M, Becher D. Global relative and absolute quantitation in microbial proteomics. Curr Opin Microbiol. 2012;15(3):364-72. Review. PMID: 22445110.

Voigt B, Hieu CX, Hempel K, Becher D, Schlüter R, Teeling H, Glöckner FO, Amann R, Hecker M, Schweder T. Cell surface proteome of the marine planctomycete Rhodopirellula baltica. Proteomics. 2012;12(11):1781-91. PMID: 22623273.

Kleiner M, Wentrup C, Lott C, Teeling H, Wetzel S, Young J, Chang YJ, Shah M, VerBerkmoes NC, Zarzycki J, Fuchs G, Markert S, Hempel K, Voigt B, Becher D, Liebeke M, Lalk M, Albrecht D, Hecker M, Schweder T, Dubilier N. Metaproteomics of a gutless marine worm and its symbiotic microbial community reveal unusual pathways for carbon and energy use. Proc Natl Acad Sci U S A. 2012;109(19):E1173-82. PMID: 22517752.

Elsholz AK, Turgay K, Michalik S, Hessling B, Gronau K, Oertel D, Mäder U, Bernhardt J, Becher D, Hecker M, Gerth U. Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis. Proc Natl Acad Sci U S A. 2012;109(19):7451-6. PMID: 22517742.

Teeling H, Fuchs BM, Becher D, Klockow C, Gardebrecht A, Bennke CM, Kassabgy M, Huang S, Mann AJ, Waldmann J, Weber M, Klindworth A, Otto A, Lange J, Bernhardt J, Reinsch C, Hecker M, Peplies J, Bockelmann FD, Callies U, Gerdts G, Wichels A, Wiltshire KH, Glöckner FO, Schweder T, Amann R. Substrate-controlled succession of marine bacterioplankton populations induced by a phytoplankton bloom. Science. 2012;336(6081):608-11. PMID: 22556258.

Palm GJ, Khanh Chi B, Waack P, Gronau K, Becher D, Albrecht D, Hinrichs W, Read RJ, Antelmann H. Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR. Nucleic Acids Res. 2012;40(9):4178-92. PMID: 22238377.

Muntel J, Hecker M, Becher D. An exclusion list based label-free proteome quantification approach using an LTQ Orbitrap. Rapid Commun Mass Spectrom. 2012;26(6):701-9. PMID: 22328225.

Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P. Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis. Science. 2012;335(6072):1103-6. PMID: 22383849.

Buescher JM, Liebermeister W, Jules M, Uhr M, Muntel J, Botella E, Hessling B, Kleijn RJ, Le Chat L, Lecointe F, Mäder U, Nicolas P, Piersma S, Rügheimer F, Becher D, Bessieres P, Bidnenko E, Denham EL, Dervyn E, Devine KM, Doherty G, Drulhe S, Felicori L, Fogg MJ, Goelzer A, Hansen A, Harwood CR, Hecker M, Hubner S, Hultschig C, Jarmer H, Klipp E, Leduc A, Lewis P, Molina F, Noirot P, Peres S, Pigeonneau N, Pohl S, Rasmussen S, Rinn B, Schaffer M, Schnidder J, Schwikowski B, Van Dijl JM, Veiga P, Walsh S, Wilkinson AJ, Stelling J, Aymerich S, Sauer U. Global network reorganization during dynamic adaptations of Bacillus subtilis metabolism. Science. 2012;335(6072):1099-103. PMID: 22383848.

Heinz E, Williams TA, Nakjang S, Noël CJ, Swan DC, Goldberg AV, Harris SR, Weinmaier T, Markert S, Becher D, Bernhardt J, Dagan T, Hacker C, Lucocq JM, Schweder T, Rattei T, Hall N, Hirt RP, Embley TM. The genome of the obligate intracellular parasite Trachipleistophora hominis: new insights into microsporidian genome dynamics and reductive evolution. PLoS Pathog. 2012;8(10):e1002979. PMID: 23133373.

2011

Chi BK, Gronau K, Mäder U, Hessling B, Becher D, Antelmann H. S-bacillithiolation protects against hypochlorite stress in Bacillus subtilis as revealed by transcriptomics and redox proteomics. Mol Cell Proteomics. 2011;10(11):M111.009506. PMID: 21749987.

Miller M, Dreisbach A, Otto A, Becher D, Bernhardt J, Hecker M, Peppelenbosch MP, van Dijl JM. Mapping of interactions between human macrophages and Staphylococcus aureus reveals an involvement of MAP kinase signaling in the host defense. J Proteome Res. 2011;10(9):4018-32. PMID: 21736355.

Lima BP, Antelmann H, Gronau K, Chi BK, Becher D, Brinsmade SR, Wolfe AJ. Involvement of protein acetylation in glucose-induced transcription of a stress-responsive promoter. Mol Microbiol. 2011;81(5):1190-204. PMID: 21696463.

Schmidl SR, Otto A, Lluch-Senar M, Piñol J, Busse J, Becher D, Stülke J. A trigger enzyme in Mycoplasma pneumoniae: impact of the glycerophosphodiesterase GlpQ on virulence and gene expression. PLoS Pathog. 2011;7(9):e1002263. PMID: 21966272.

Elsholz AK, Hempel K, Michalik S, Gronau K, Becher D, Hecker M, Gerth U. Activity control of the ClpC adaptor McsB in Bacillus subtilis. J Bacteriol. 2011;193(15):3887-93. PMID: 21622759.

Markert S, Gardebrecht A, Felbeck H, Sievert SM, Klose J, Becher D, Albrecht D, Thürmer A, Daniel R, Kleiner M, Hecker M, Schweder T. Status quo in physiological proteomics of the uncultured Riftia pachyptila endosymbiont. Proteomics. 2011;11(15):3106-17. PMID: 21710568.

Delumeau O, Lecointe F, Muntel J, Guillot A, Guédon E, Monnet V, Hecker M, Becher D, Polard P, Noirot P. The dynamic protein partnership of RNA polymerase in Bacillus subtilis. Proteomics. 2011;11(15):2992-3001. PMID: 21710567.

Becher D, Büttner K, Moche M, Hessling B, Hecker M. From the genome sequence to the protein inventory of Bacillus subtilis. Proteomics. 2011;11(15):2971-80. Review. PMID: 21710564.

Dreisbach A, van der Kooi-Pol MM, Otto A, Gronau K, Bonarius HP, Westra H, Groen H, Becher D, Hecker M, van Dijl JM. Surface shaving as a versatile tool to profile global interactions between human serum proteins and the Staphylococcus aureus cell surface. Proteomics. 2011;11(14):2921-30. PMID: 21674804.

Albaum SP, Hahne H, Otto A, Haußmann U, Becher D, Poetsch A, Goesmann A, Nattkemper TW. A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study. Proteome Sci. 2011;9:30. PMID: 21663690.

Kohlmann Y, Pohlmann A, Otto A, Becher D, Cramm R, Lütte S, Schwartz E, Hecker M, Friedrich B. Analyses of soluble and membrane proteomes of Ralstonia eutropha H16 reveal major changes in the protein complement in adaptation to lithoautotrophy. J Proteome Res. 2011;10(6):2767-76. PMID: 21561103.

Eymann C, Schulz S, Gronau K, Becher D, Hecker M, Price CW. In vivo phosphorylation patterns of key stressosome proteins define a second feedback loop that limits activation of Bacillus subtilis σB. Mol Microbiol. 2011;80(3):798-810. PMID: 21362065.

Tefon BE, Maass S, Ozcengiz E, Becher D, Hecker M, Ozcengiz G. A comprehensive analysis of Bordetella pertussis surface proteome and identification of new immunogenic proteins. Vaccine. 2011;29(19):3583-95. PMID: 21397717.

Maass S, Sievers S, Zühlke D, Kuzinski J, Sappa PK, Muntel J, Hessling B, Bernhardt J, Sietmann R, Völker U, Hecker M, Becher D. Efficient, global-scale quantification of absolute protein amounts by integration of targeted mass spectrometry and two-dimensional gel-based proteomics. Anal Chem. 2011;83(7):2677-84. PMID: 21395229.

Hempel K, Herbst FA, Moche M, Hecker M, Becher D. Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions. J Proteome Res. 2011;10(4):1657-66. PMID: 21323324.

Yıldırım V, Ozcan S, Becher D, Büttner K, Hecker M, Ozcengiz G. Characterization of proteome alterations in Phanerochaete chrysosporium in response to lead exposure. Proteome Sci. 2011;9:12. PMID: 21388532.

Elsholz AK, Hempel K, Pöther DC, Becher D, Hecker M, Gerth U. CtsR inactivation during thiol-specific stress in low GC, Gram+ bacteria. Mol Microbiol. 2011;79(3):772-85. PMID: 21208299.

Saller MJ, Otto A, Berrelkamp-Lahpor GA, Becher D, Hecker M, Driessen AJ. Bacillus subtilis YqjG is required for genetic competence development. Proteomics. 2011;11(2):270-82. PMID: 21204254.

2010

Price CE, Otto A, Fusetti F, Becher D, Hecker M, Driessen AJ. Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions. Proteomics. 2010;10(18):3235-47. PMID: 20706981.

Dreisbach A, Hempel K, Buist G, Hecker M, Becher D, van Dijl JM. Profiling the surfacome of Staphylococcus aureus. Proteomics. 2010;10(17):3082-96. PMID: 20662103.

Chi BK, Albrecht D, Gronau K, Becher D, Hecker M, Antelmann H. The redox-sensing regulator YodB senses quinones and diamide via a thiol-disulfide switch in Bacillus subtilis. Proteomics. 2010;10(17):3155-64. PMID: 20652907.

Hyyryläinen HL, Marciniak BC, Dahncke K, Pietiäinen M, Courtin P, Vitikainen M, Seppala R, Otto A, Becher D, Chapot-Chartier MP, Kuipers OP, Kontinen VP. Penicillin-binding protein folding is dependent on the PrsA peptidyl-prolyl cis-trans isomerase in Bacillus subtilis. Mol Microbiol. 2010;77(1):108-27. PMID: 20487272.

Möbius K, Arias-Cartin R, Breckau D, Hännig AL, Riedmann K, Biedendieck R, Schröder S, Becher D, Magalon A, Moser J, Jahn M, Jahn D. Heme biosynthesis is coupled to electron transport chains for energy generation. Proc Natl Acad Sci U S A. 2010;107(23):10436-41. PMID: 20484676.

Schmidl SR, Gronau K, Pietack N, Hecker M, Becher D, Stülke J. The phosphoproteome of the minimal bacterium Mycoplasma pneumoniae: analysis of the complete known Ser/Thr kinome suggests the existence of novel kinases. Mol Cell Proteomics. 2010;9(6):1228-42. PMID: 20097688.

Sievers S, Ernst CM, Geiger T, Hecker M, Wolz C, Becher D, Peschel A. Changing the phospholipid composition of Staphylococcus aureus causes distinct changes in membrane proteome and membrane-sensory regulators. Proteomics. 2010;10(8):1685-93. PMID: 20162562.

Hempel K, Pané-Farré J, Otto A, Sievers S, Hecker M, Becher D. Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach. J Proteome Res. 2010;9(3):1579-90. PMID: 20108986.

Miller M, Donat S, Rakette S, Stehle T, Kouwen TR, Diks SH, Dreisbach A, Reilman E, Gronau K, Becher D, Peppelenbosch MP, van Dijl JM, Ohlsen K. Staphylococcal PknB as the first prokaryotic representative of the proline-directed kinases. PLoS One. 2010;5(2):e9057. PMID: 20140229.

Hecker M, Becher D, Fuchs S, Engelmann S. A proteomic view of cell physiology and virulence of Staphylococcus aureus. Int J Med Microbiol. 2010;300(2-3):76-87 Review. PMID: 20005169.

Hahne H, Mäder U, Otto A, Bonn F, Steil L, Bremer E, Hecker M, Becher D. A comprehensive proteomics and transcriptomics analysis of Bacillus subtilis salt stress adaptation. J Bacteriol. 2010;192(3):870-82. PMID: 19948795.

Schmidl SR, Gronau K, Hames C, Busse J, Becher D, Hecker M, Stülke J. The stability of cytadherence proteins in Mycoplasma pneumoniae requires activity of the protein kinase PrkC. Infect Immun. 2010 Jan;78(1):184-92. PMID: 19858294.

Pietack N, Becher D, Schmidl SR, Saier MH, Hecker M, Commichau FM, Stülke J. In vitro phosphorylation of key metabolic enzymes from Bacillus subtilis: PrkC phosphorylates enzymes from different branches of basic metabolism. J Mol Microbiol Biotechnol. 2010;18(3):129-40. PMID: 20389117.

Otto A, Bernhardt J, Meyer H, Schaffer M, Herbst FA, Siebourg J, Mäder U, Lalk M, Hecker M, Becher D. Systems-wide temporal proteomic profiling in glucose-starved Bacillus subtilis. Nat Commun. 2010;1:137. PMID: 21266987.

2009

Becher D, Hempel K, Sievers S, Zühlke D, Pané-Farré J, Otto A, Fuchs S, Albrecht D, Bernhardt J, Engelmann S, Völker U, van Dijl JM, Hecker M. A proteomic view of an important human pathogen–towards the quantification of the entire Staphylococcus aureus proteome. PLoS One. 2009;4(12):e8176. PMID: 19997597.

Pöther DC, Liebeke M, Hochgräfe F, Antelmann H, Becher D, Lalk M, Lindequist U, Borovok I, Cohen G, Aharonowitz Y, Hecker M. Diamide triggers mainly S Thiolations in the cytoplasmic proteomes of Bacillus subtilis and Staphylococcus aureus. J Bacteriol. 2009;191(24):7520-30. PMID: 19837798.

Hempel K, Rosen R, Becher D, Büttner K, Hecker M, Ron EZ. Analysis of ultra acidic proteins by the use of anodic acidic gels. Anal Biochem. 2009;385(2):208-14. PMID: 19084495.

Altindiş E, Tefon BE, Yildirim V, Ozcengiz E, Becher D, Hecker M, Ozcengiz G. Immunoproteomic analysis of Bordetella pertussis and identification of new immunogenic proteins. Vaccine. 2009;27(4):542-8. PMID: 19028538.

Hahne H, Wolff S, Hecker M, Becher D. From complementarity to comprehensiveness–targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics. 2008;8(19):4123-36. PMID: 18763711.

Liebeke M, Pöther DC, van Duy N, Albrecht D, Becher D, Hochgräfe F, Lalk M, Hecker M, Antelmann H. Depletion of thiol-containing proteins in response to quinones in Bacillus subtilis. Mol Microbiol. 2008;69(6):1513-29. PMID: 18673455.

Wolff S, Hahne H, Hecker M, Becher D. Complementary analysis of the vegetative membrane proteome of the human pathogen Staphylococcus aureus. Mol Cell Proteomics. 2008;7(8):1460-8. PMID: 18460691.

Dreisbach A, Otto A, Becher D, Hammer E, Teumer A, Gouw JW, Hecker M, Völker U. Monitoring of changes in the membrane proteome during stationary phase adaptation of Bacillus subtilis using in vivo labeling techniques. Proteomics. 2008;8(10):2062-76. PMID: 18491319.

Zweers JC, Barák I, Becher D, Driessen AJ, Hecker M, Kontinen VP, Saller MJ, Vavrová L, van Dijl JM. Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes. Microb Cell Fact. 2008;7:10. PMID: 18394159.

Hieu CX, Voigt B, Albrecht D, Becher D, Lombardot T, Glöckner FO, Amann R, Hecker M, Schweder T. Detailed proteome analysis of growing cells of the planctomycete Rhodopirellula baltica SH1T. Proteomics. 2008;8(8):1608-23. PMID: 18340632.

Leelakriangsak M, Huyen NT, Töwe S, van Duy N, Becher D, Hecker M, Antelmann H, Zuber P. Regulation of quinone detoxification by the thiol stress sensing DUF24/MarR-like repressor, YodB in Bacillus subtilis. Mol Microbiol. 2008;67(5):1108-24. PMID: 18208493.

2007

Eymann C, Becher D, Bernhardt J, Gronau K, Klutzny A, Hecker M. Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis. Proteomics. 2007;7(19):3509-26. PMID: 17726680.

Hochgräfe F, Mostertz J, Pöther DC, Becher D, Helmann JD, Hecker M. S-cysteinylation is a general mechanism for thiol protection of Bacillus subtilis proteins after oxidative stress. J Biol Chem. 2007;282(36):25981-5. PMID: 17611193.

Wolff S, Antelmann H, Albrecht D, Becher D, Bernhardt J, Bron S, Büttner K, van Dijl JM, Eymann C, Otto A, Tam le T, Hecker M. Towards the entire proteome of the model bacterium Bacillus subtilis by gel-based and gel-free approaches. J Chromatogr B Analyt Technol Biomed Life Sci. 2007;849(1-2):129-40. Review. PMID: 17055787.

Ozcan S, Yildirim V, Kaya L, Albrecht D, Becher D, Hecker M, Ozcengiz G. Phanerochaete chrysosporium soluble proteome as a prelude for the analysis of heavy metal stress response. Proteomics. 2007;7(8):1249-60. PMID: 17366474.

Markert S, Arndt C, Felbeck H, Becher D, Sievert SM, Hügler M, Albrecht D, Robidart J, Bench S, Feldman RA, Hecker M, Schweder T. Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila. Science. 2007;315(5809):247-50. PMID: 17218528.

Borriss M, Lombardot T, Glöckner FO, Becher D, Albrecht D, Schweder T. Genome and proteome characterization of the psychrophilic Flavobacterium bacteriophage 11b. Extremophiles. 2007;11(1):95-104. PMID: 16932843.

2006 and older

Linsen L, Löcherbach J, Berth M, Becher D, Bernhardt J. Visual analysis of gel-free proteome data. IEEE Trans Vis Comput Graph. 2006;12(4):497-508. PMID: 16805259.

Wolff S, Otto A, Albrecht D, Zeng JS, Büttner K, Glückmann M, Hecker M, Becher D. Gel-free and gel-based proteomics in Bacillus subtilis: a comparative study. Mol Cell Proteomics. 2006;5(7):1183-92. PMID: 16552027.

Mattow J, Siejak F, Hagens K, Becher D, Albrecht D, Krah A, Schmidt F, Jungblut PR, Kaufmann SH, Schaible UE. Proteins unique to intraphagosomally grown Mycobacterium tuberculosis. Proteomics. 2006;6(8):2485-94. PMID: 16548060.

Kohler C, Wolff S, Albrecht D, Fuchs S, Becher D, Büttner K, Engelmann S, Hecker M. Proteome analyses of Staphylococcus aureus in growing and non-growing cells: a physiological approach. Int J Med Microbiol. 2005;295(8):547-65. PMID: 16325551.

Wesche J, Hammer E, Becher D, Burchhardt G, Schauer F. The bphC gene-encoded 2,3-dihydroxybiphenyl-1,2-dioxygenase is involved in complete degradation of dibenzofuran by the biphenyl-degrading bacterium Ralstonia sp. SBUG 290. J Appl Microbiol. 2005;98(3):635-45. PMID: 15715866.

Rosen R, Becher D, Büttner K, Biran D, Hecker M, Ron EZ. Probing the active site of homoserine trans-succinylase. FEBS Lett. 2004;577(3):386-92. PMID: 15556615.

Rosen R, Becher D, Büttner K, Biran D, Hecker M, Ron EZ. Highly phosphorylated bacterial proteins. Proteomics. 2004;4(10):3068-77. PMID: 15378765.

Eymann C, Dreisbach A, Albrecht D, Bernhardt J, Becher D, Gentner S, Tam le T, Büttner K, Buurman G, Scharf C, Venz S, Völker U, Hecker M. A comprehensive proteome map of growing Bacillus subtilis cells. Proteomics. 2004;4(10):2849-76. PMID: 15378759.

Ziebandt AK, Becher D, Ohlsen K, Hacker J, Hecker M, Engelmann S. The influence of agr and sigmaB in growth phase dependent regulation of virulence factors in Staphylococcus aureus. Proteomics. 2004 ;4(10):3034-47. PMID: 15378746.

Voigt B, Schweder T, Becher D, Ehrenreich A, Gottschalk G, Feesche J, Maurer KH, Hecker M. A proteomic view of cell physiology of Bacillus licheniformis. Proteomics. 2004;4(5):1465-90. PMID: 15188415.

Weber H, Engelmann S, Becher D, Hecker M. Oxidative stress triggers thiol oxidation in the glyceraldehyde-3-phosphate dehydrogenase of Staphylococcus aureus. Mol Microbiol. 2004;52(1):133-40. PMID: 15049816.

Rosen R, Sacher A, Shechter N, Becher D, Büttner K, Biran D, Hecker M, Ron EZ. Two-dimensional reference map of Agrobacterium tumefaciens proteins. Proteomics. 2004;4(4):1061-73. PMID: 15048987.

Brandt R, Nawka M, Kellermann J, Salazar R, Becher D, Krantz S. Nucleophosmin is a component of the fructoselysine-specific receptor in cell membranes of Mono Mac 6 and U937 monocyte-like cells. Biochim Biophys Acta. 2004;1670(2):132-6. PMID: 14738996.

Krah A, Schmidt F, Becher D, Schmid M, Albrecht D, Rack A, Büttner K, Jungblut PR. Analysis of automatically generated peptide mass fingerprints of cellular proteins and antigens from Helicobacter pylori 26695 separated by two-dimensional electrophoresis. Mol Cell Proteomics. 2003;2(12):1271-83. PMID: 14519719.

Rosen R, Matthysse AG, Becher D, Biran D, Yura T, Hecker M, Ron EZ. Proteome analysis of plant-induced proteins of Agrobacterium tumefaciens. FEMS Microbiol Ecol. 2003;44(3):355-60. PMID: 19719616.

Bandow JE, Becher D, Büttner K, Hochgräfe F, Freiberg C, Brötz H, Hecker M. The role of peptide deformylase in protein biosynthesis: a proteomic study. Proteomics. 2003;3(3):299-306. PMID: 12627383.

Stope MB, Becher D, Hammer E, Schauer F. Cometabolic ring fission of dibenzofuran by Gram-negative and Gram-positive biphenyl-utilizing bacteria. Appl Microbiol Biotechnol. 2002;59(1):62-7. PMID: 12073133.

Rosen R, Büttner K, Becher D, Nakahigashi K, Yura T, Hecker M, Ron EZ. Heat shock proteome of Agrobacterium tumefaciens: evidence for new control systems. J Bacteriol. 2002;184(6):1772-8. PMID: 11872730.

Rosen R, Biran D, Gur E, Becher D, Hecker M, Ron EZ. Protein aggregation in Escherichia coli: role of proteases. FEMS Microbiol Lett. 2002;207(1):9-12. PMID: 11886743.

Becher D, Specht M, Hammer E, Francke W, Schauer F. Cometabolic degradation of dibenzofuran by biphenyl-cultivated Ralstonia sp. strain SBUG 290. Appl Environ Microbiol. 2000;66(10):4528-31. PMID: 11010910.

Scheller U, Zimmer T, Becher D, Schauer F, Schunck WH. Oxygenation cascade in conversion of n-alkanes to alpha,omega-dioic acids catalyzed by cytochrome P450 52A3. J Biol Chem. 1998;273(49):32528-34. PMID: 9829987.

Hundt K, Wagner M, Becher D, Hammer E, Schauer F. Effect of selected environmental factors on degradation and mineralization of biaryl compounds by the bacterium Ralstonia pickettii in soil and compost. Chemosphere. 1998;36(10):2321-35. PMID: 9566302.