Prof. Dr. Dörte Becher

Executive Vice Director

Chair of Microbial Proteomics

Felix-Hausdorff-Straße 8 - 1.floor - room-nr.: 1.56
17489 Greifswald

phone: +49 (0)3834 420 5903
fax:      +49 (0)3834 420 5994
email:  dbecher(at)uni-greifswald(dot)de

opening hours: only by appointment

Curriculum Vitae
since 05/2020 Managing Director of the “Institute of Microbiology” at the University of Greifswald
10/2014 – 04/2020 Managing Vice Director of the Institute of Microbiology, Ernst-Moritz-Arndt University Greifswald
since 06/2013 Professor for Microbial Proteomics at the Institute for Microbiology, Ernst-Moritz-Arndt University Greifswald
01/2012 Habilitation in Microbiology, Topic “Novel qualitative and quantitative approaches for global proteome analysis in gram positive bacteria”
2003 – 2013 Head of the Group “Microbial Proteomics and Mass Spectrometry” at the Institute for Microbiology, Ernst-Moritz-Arndt University Greifswald
1999 – 2013 Scientific Assistant, Institute for Microbiology, in the group of Prof. M. Hecker, Ernst-Moritz-Arndt University Greifswald
1993 – 1998 Ph.D. (Dr. rer. nat., Microbiology) Ernst-Moritz-Arndt University Greifswald, Institute of Microbiology, in the group of Prof. F. Schauer
1992 University of Aberdeen, ERASMUS Students Exchange Program
1987 – 1992 Study of Chemistry, Diploma, Ernst-Moritz-Arndt University Greifswald
Further Activities
since 2011 Secretary-Treasurer of the Bacteriology and Applied Microbiology Division (BAM) of the International Union of Microbiological Societies (IUMS)
Research Interests
  • Development, establishment and optimization of mass spectrometry based proteomics methods with a focus on global characterization of microbial proteomes in a qualitative and quantitative manner
  • Application of newly established methods for the global analysis of protein expression profiles as well as the characterization of single proteins, post translational modified proteins or protein complexes
  • Absolute protein quantification with targeted as well as global methods for determination of proteins on the level of copy number per cells especially in the context of systems biology
  • Elaboration and application of proteomics methods with particular consideration of technologically/industrial relevant questions, infection related conditions or meta-proteomics aspects.


Selected Funded Projects

POMPU - Proteogenomic of Marine Polysaccharid Utilization

Project B2 “In situ mechanisms of polysaccharide degradation of key bacteroidetal genera in spring algae blooms” together with Dr. Bernhard Fuchs (MPI Marine Microbiology Bremen),   funded by the German Research Foundation from 2016 to 2019.

DFG Priority Programme 2002 - Small Proteins in Prokaryotes, an Unexplored World

Project Z Proteomics and Peptidomics for the identification and functional characterization of sORF coded proteins” together with Prof. Dr. Andreas Tholey (Christian-Albrechts-Universität Kiel), funded by the German Research Foundation from 2017 to 2020.

CoInfect - Deciphering the Pathomechanisms of Bacterial-Viral Coinfections With New Biomedical Models

This project is funded as part of the excellence initiative of the Federal State Mecklenburg-Pomerania funded with money of the European Social Fund (ESF). In frame of this cooperative project our group focus on the establishment, optimization, and application of Ms-based analysis of ubiquitination. The project is funded from January 2017 to March 2021.

MoMV - Modulare Oral Applicable Multi-Vaccines – Principal Solutions

This cooperative project aims at development of a new system for the vaccination of fish. Our group contributes result on the application of outer membrane vesicles as possible vaccine cargo. The project is funded by the European Maritime and Fisheries Fund (EMFF) and the state Mecklenburg-Vorpommern from July 2017 to July 2020.

European Regional Development Fund (ERDF) - EASY-nLC 1200 Nano-HPLC

With the support from the European Regional Development Fund (ERDF) it was possible to acquire a nano- HPLC System for the mass spectrometric analysis of complex peptide samples generated in proteomics approaches. The LC- System will be used within the frame of extramurally funded research projects of applied sciences as well as in direct cooperations with industrial partners.

European Regional Development Fund (ERDF) - High-Performance Workstation With the Software "Scaffold"

With the support of the European Regional Development Fund (ERDF) it was possible to acquire a high- performance workstation with the batch-licensed software "Scaffold". The system will be used to analyze and exchange data within the frame of industrial cooperation.

Publication list
  • Walgraeve J, Ferrero-Bordera B, Maaß S, Becher D, Schwerdtfeger R, van Dijl JM, Seefried M. Diamide-based screening method for the isolation of improved oxidative stress tolerance phenotypes in Bacillus mutant libraries. Microbiol Spectr. 2023 Oct 11:e0160823. doi: 10.1128/spectrum.01608-23. Online ahead of print. PMID: 37819171.
  • Öktem A, Núñez-Nepomuceno D, Ferrero-Bordera B, Walgraeve J, Seefried M, Gesell Salazar M, Steil L, Michalik S, Maaß S, Becher D, Mäder U, Völker U, van Dijl JM. Enhancing bacterial fitness and recombinant enzyme yield by engineering the quality control protease HtrA of Bacillus subtilis. Microbiol Spectr. 2023 Oct 11:e0177823. doi: 10.1128/spectrum.01778-23. Online ahead of print. PMID: 37819116.
  • Meene A, Gierse L, Schwaiger T, Karte C, Schröder C, Höper D, Wang H, Groß V, Wünsche C, Mücke P, Kreikemeyer B, Beer M, Becher D, Mettenleiter TC, Riedel K, Urich T. Archaeome structure and function of the intestinal tract in healthy and H1N1 infected swine. Front Microbiol. 2023 Sep 13;14:1250140. doi: 10.3389/fmicb.2023.1250140. eCollection 2023. PMID: 37779690.
  • Kromrey ML, Oswald S, Becher D, Bartel J, Schulze J, Paland H, Ittermann T, Hadlich S, Kühn JP, Mouchantat S. Intracerebral gadolinium deposition following blood-brain barrier disturbance in two different mouse models. Sci Rep. 2023 Jun 22;13(1):10164. doi: 10.1038/s41598-023-36991-8. PMID: 37349374.
  • Alvarenga-Lucius L, Linhartová M, Schubert H, Maaß S, Becher D, Hess WR, Sobotka R, Hagemann M. The high-light-induced protein SliP4 binds to NDH1 and photosystems facilitating cyclic electron transport and state transition in Synechocystis sp. PCC 6803. New Phytol. 2023 Jun 6. PMID: 37282607.
  • Hüsler D, Stauffer P, Keller B, Böck D, Steiner T, Ostrzinski A, Vormittag S, Striednig B, Swart AL, Letourneur F, Maaß S, Becher D, Eisenreich W, Pilhofer M, Hilbi H. The large GTPase Sey1/atlastin mediates lipid droplet- and FadL-dependent intracellular fatty acid metabolism of Legionella pneumophila. Elife. 2023 May 9;12:e85142. PMID: 37158597.
  • Dutschei T, Beidler I, Bartosik D, Seeßelberg JM, Teune M, Bäumgen M, Ferreira SQ, Heldmann J, Nagel F, Krull J, Berndt L, Methling K, Hein M, Becher D, Langer P, Delcea M, Lalk M, Lammers M, Höhne M, Hehemann JH, Schweder T, Bornscheuer UT. Marine Bacteroidetes enzymatically digest xylans from terrestrial plants. Environ Microbiol. 2023 Apr 30.  PMID: 37121608.
  • Gierse LC, Meene A, Skorka S, Cuypers F, Surabhi S, Ferrero-Bordera B, Kreikemeyer B, Becher D, Hammerschmidt S, Siemens N, Urich T, Riedel K. Impact of pneumococcal and viral pneumonia on the respiratory and intestinal tract microbiomes of mice. Microbiol Spectr. 2023 Mar 29:e0344722. PMID: 36988458.
  • Hadjeras L, Heiniger B, Maaß S, Scheuer R, Gelhausen R, Azarderakhsh S, Barth-Weber S, Backofen R, Becher D, Ahrens CH, Sharma CM, Evguenieva-Hackenberg E. Unraveling the small proteome of the plant symbiont Sinorhizobium meliloti by ribosome profiling and proteogenomics. Microlife. 2023 Mar 10;4:uqad012. PMID: 37223733.
  • Maaß S, Antelo-Varela M, Bonn F, Becher D. Sample preparation for mass spectrometry-based absolute quantification of bacterial proteins in antibiotic stress research. Methods Mol Biol. 2023;2601:335-348. PMID: 36445593.
  • Hadjeras L, Bartel J, Maier LK, Maaß S, Vogel V, Svensson SL, Eggenhofer F, Gelhausen R, Müller T, Alkhnbashi OS, Backofen R, Becher D, Sharma CM, Marchfelder A. Revealing the small proteome of Haloferax volcanii by combining ribosome profiling and small-protein optimized mass spectrometry. Microlife. 2023 Jan 16;4:uqad001. PMID: 37223747.
  • Matilla MA, Genova R, Martín-Mora D, Maaβ S, Becher D, Krell T. The cellular abundance of chemoreceptors, chemosensory signaling proteins, sensor histidine kinases, and solute binding proteins of Pseudomonas aeruginosa provides insight into sensory preferences and signaling mechanisms. Int J Mol Sci. 2023 Jan 10;24(2):1363.  PMID: 36674894.
  • Fu Y, Maaβ S, Cavallo FM, de Jong A, Raangs E,Westra J,  Buist G,Becher D,  van Dijl JM. Differential virulence of aggregatibacter actinomycetemcomitans serotypes explained by exoproteome heterogeneity. Microbiol Spectr. 2023;11(1):e0329822. PMID: 36541765
  • Vormittag S, Hüsler D, Haneburger I, Kroniger T, Anand A, Prantl M, Barisch C, Maaß S, Becher D, Letourneur F, Hilbi H. Legionella- and host-driven lipid flux at LCV-ER membrane contact sites promotes vacuole remodeling. EMBO Rep. 2023 Jan 2:e56007. PMID: 36588479.
  • Beidler I, Robb CS, Vidal-Melgosa S, Zühlke M-K, Bartosik D, Solanki V, Markert S, Becher D, Schweder T, Hehemann J-H (2022). Marine bacteroidetes use a conserved enzymatic cascade to digest diatom β-mannan. ISME J 2022 Nov 21. PMID: 36411326.

  • Chiriches C, Nicolaisen N, Wieske M, Elhaddad H, Mehmetbeyoglu E, Alvares C, Becher D, Hole P, Gerhard Ottmann O, Ruthardt M (2022). Understanding a high-risk acute myeloid leukemia by analyzing the interactome of its major driver mutation. PLoS Genet 18, e1010463. PMID: 36288392.

  • Dutschei, T, Zühlke M-K, Welsch N, Eisenack T, Hilkmann M, Krull J, Stühle C, Brott S, Dürwald A, Reisky L, Hehemann, J-H, Becher D, Schweder T, Bornscheuer UT(2022). Metabolic engineering enables Bacillus licheniformis to grow on the marine polysaccharide ulvan. Microb Cell Fact 21, 207. PMID: 36217189.

  • Matilla MA, Udaondo Z, Maaß S, Becher D, Krell T (2022). Virulence induction in Pseudomonas aeruginosa under inorganic phosphate limitation: a proteomics perspective. Microbiol Spectr, e0259022. PMID: 3635431.

  • Kroniger T, Mehanny M, Schlüter R, Trautwein-Schult A, Köllner B, Becher D. Effect of Iiron limitation, elevated temperature, and florfenicol on the proteome and vesiculation of the fish pathogen Aeromonas salmonicida. Microorganisms. 2022 Aug 27;10(9):1735. PMID: 36144337.

  • du Teil Espina M, Fu Y, van der Horst D, Hirschfeld C, López-Álvarez M, Mulder LM, Gscheider C, Haider Rubio A, Huitema M, Becher D, Heeringa P, van Dijl JM. Coating and Corruption of Human Neutrophils by Bacterial Outer Membrane Vesicles. Microbiol Spectr. 2022 Oct 26;10(5):e0075322. doi: 10.1128/spectrum.00753-22. Epub 2022 Aug 24. PMID: 36000865.

  • Sasson G, Moraïs S, Kokou F, Plate K, Trautwein-Schult A, Jami E, Bayer EA, Becher D, Mizrahi I. Metaproteome plasticity sheds light on the ecology of the rumen microbiome and its connection to host traits. ISME J. 2022 Nov;16(11):2610-2621. doi: 10.1038/s41396-022-01295-8. Epub 2022 Aug 16. PMID: 35974086.

  • Fu Y, Maaβ S, du Teil Espina M, Wolters AHG, Gong Y, de Jong A, Raangs E, Buist G, Westra J, Becher D, van Dijl JM. Connections between Exoproteome Heterogeneity and Virulence in the Oral Pathogen Aggregatibacter actinomycetemcomitansmSystems. 2022 Jun 28;7(3):e0025422. doi: 10.1128/msystems.00254-22. Epub 2022 Jun 13. PMID: 35695491.

  • Song, K, Hagemann, M, Georg, J, Maaß, S, Becher, D and Hess, WR (2022). Expression of the cyanobacterial FoF1 ATP synthase regulator AtpΘ depends on small DNA-binding proteins and differential mRNA stability. Microbiol Spectr, e0256221. PMID: 35446123.

  • Hochstrasser, R, Michaelis, S, Brülisauer, S, Sura, T, Fan, M, Maaß, S, et al. (2022). Migration of Acanthamoeba through Legionella biofilms is regulated by the bacterial Lqs-LvbR network, effector proteins and the flagellum. Environ Microbiol. PMID: 35415862.
  • Mehanny, M, Kroniger, T, Koch, M, Hoppstädter, J, Becher, D, Kiemer, AK, et al. (2022). Yields and immunomodulatory effects of pneumococcal membrane vesicles differ with the bacterial growth phase. Adv Healthc Mater 11, e2101151. PMID: 34724354.
  • Pudlo, NA, Pereira, GV, Parnami, J, Cid, M, Markert, S, Tingley, JP, et al. (2022). Diverse events have transferred genes for edible seaweed digestion from marine to human gut bacteria. Cell Host Microbe 30, 314-328.e11. PMID: 35240043.
  • Sura, T, Gering, V, Cammann, C, Hammerschmidt, S, Maaß, S, Seifert, U, et al. (2022). Streptococcus pneumoniae and Influenza A Virus co-infection induces altered polyubiquitination in A549 Cells. Front Cell Infect Microbiol 12, 817532. PMID: 35281454.
  • Kroniger T, Flender D, Schlüter R, Köllner B, Trautwein-Schult A, Becher D. Proteome analysis of the Gram-positive fish pathogen Renibacterium salmoninarum reveals putative role of membrane vesicles in virulence. Sci Rep. 2022 Feb 22;12(1):3003. doi: 10.1038/s41598-022-06130-w. PMID: 35194033.
  • Kloska A, Cech GM, Nowicki D, Maciąg-Dorszyńska M, Bogucka AE, Markert S, Becher D, Potrykus K, Czaplewska P, Szalewska-Pałasz A. Three microbial musketeers of the seas: Shewanella baltica, Aliivibriofischeri and Vibrio harveyi, and their adaptation to different salinity probed by a proteomic approach. Int J Mol Sci. 2022 Jan 6;23(2):619. doi: 10.3390/ijms23020619. PMID: 35054801.
  • Sura T, Surabhi S, Maaß S, Hammerschmidt S, Siemens N, Becher D. The global proteome and ubiquitinome of bacterial and viral co-infected bronchial epithelial cells. J Proteomics. 2022 Jan 6; 250:104387. PMID: 34600154.
  • Mehanny M, Kroniger T, Koch M, Hoppstädter J, Becher D, Kiemer AK, Lehr CM, Fuhrmann G. Yields and immunomodulatory effects of pneumococcal membrane vesicles differ with the bacterial growth phase. Adv Healthc Mater. 2021 Nov 1:e2101151. PMID: 34724354.

  • Gierse LC, Meene A, Schultz D, Schwaiger T, Schröder C, Mücke P, Zühlke D, Hinzke T, Wang H, Methling K, Kreikemeyer B, Bernhardt J, Becher D, Mettenleiter TC, Lalk M, Urich T, Riedel K. Influenza A H1N1 induced disturbance of the respiratory and fecal microbiome of german landrace pigs - a multi-Omics characterization. Microbiol Spectr. 2021 Oct 31;9(2):e0018221. PMID: 34612695.

  • Sura T, Surabhi S, Maaß S, Hammerschmidt S, Siemens N, Becher D. The global proteome and ubiquitinome of bacterial and viral co-infected bronchial epithelial cells. J Proteomics. 2022 Jan 6; 250:104387. PMID: 34600154.

  • Graf AC, Striesow J, Pané-Farré J, Sura T, Wurster M, Lalk M, Pieper DH, Becher D, Kahl BC, Riedel K. An innovative protocol for metaproteomic analyses of microbial pathogens in cystic fibrosis sputum. Front Cell Infect Microbiol. 2021 Aug 27; 11:724569. PMID: 34513734

  • Song K, Baumgartner D, Hagemann M, Muro-Pastor AM, Maaß S, Becher D, Hess WR. AtpΘ is an inhibitor of F0F1 ATP synthase to arrest ATP hydrolysis during low-energy conditions in cyanobacteria. Curr Biol. 2021 Nov 5:S0960-9822(21)01495-0. PMID: 34762820.

  • Liao Y, Vogel V, Hauber S, Bartel J, Alkhnbashi OS, Maaß S, Schwarz TS, Backofen R, Becher D, Duggin IG, Marchfelder A. CdrS is a global transcriptional regulator influencing cell division in Haloferax volcanii. mBio. 2021 Jul 13:e0141621. PMID: 34253062.

  • Biran D, Sura T, Otto A, Yair Y, Becher D, Ron EZ. Surviving serum - the E. coliiss gene (increased serum survival) of extraintestinal pathogenic E. coli (ExPEC) is required for the synthesis of group 4 capsule. Infect Immun. 2021 Jun 28:IAI0031621. PMID: 34181459.

  • Brauer M, Lassek C, Hinze C, Hoyer J, Becher D, Jahn D, Sievers S, Riedel K. What's a biofilm?-How the choice of the biofilm modeli the protein inventory of Clostridioides difficile. Front Microbiol. 2021 Jun 10;12:682111. PMID: 34177868.

  • Märkle P, Maier LK, Maaß S, Hirschfeld C, Bartel J, Becher D, Voß B, Marchfelder A. A small RNA is linking CRISPR-Cas and zinc transport. Front Mol Biosci. 2021 May 13. PMID: 34055875.

  • Cassidy L, Kaulich PT, Maaß S, Bartel J, Becher D, Tholey A. Bottom-up and top-down proteomic approaches for the identification, characterization and quantification of the low molecular weight proteome with focus on short open reading frame-encoded peptides. Proteomics. 2021 Jun 19. PMID: 34145981.

  • Ünsaldı E, Kurt-Kızıldoğan A, Özcan S, Becher D, Voigt B, Aktaş C, Özcengiz G. Proteomic analysis of a hom-disrupted, cephamycin C overproducing Streptomyces clavuligerusProtein Pept Lett. 2021. PMID: 32707026.

  • Trautwein-Schult A, Bartel J, Maaß S, Becher D. Metabolic labeling of Clostridioides difficile proteins. Methods Mol Biol. 2021. PMID: 33950497.

  • Francis TB, Bartosik D, Sura T, Sichert A, Hehemann JH, Markert S, Schweder T, Fuchs BM, Teeling H, Amann RI, Becher D. Changing expression patterns of TonB-dependent transporters suggest shifts in polysaccharide consumption over the course of a spring phytoplankton bloom. ISME J. 2021 Mar 1. PMID: 33649555.

  • Dürwald A, Zühlke MK, Schlüter R, Gebbe R, Bartosik D, Unfried F, Becher D, Schweder T. Reaching out in anticipation: bacterial membrane extensions represent a permanent investment in polysaccharide sensing and utilization. Environ Microbiol. 2021 Apr 19. PMID: 33876569.

  • Nepal S, Maaß S, Grasso S, Cavallo FM, Bartel J, Becher D, Bathoorn E, van Dijl JM. Proteomic charting of imipenem adaptive responses in a highly carbapenem resistant clinical Enterobacter roggenkampii isolate. Antibiotics (Basel). 2021 Apr 28. PMID: 33924830.

  • Oberbeckmann S, Bartosik D, Huang S, Werner J, Hirschfeld C, Wibberg D, Heiden SE, Bunk B, Overmann J, Becher D, Kalinowski J, Schweder T, Labrenz M, Markert S. Genomic and proteomic profiles of biofilms on microplastics are decoupled from artificial surface properties. Environ Microbiol. 2021 Apr 19. PMID: 33876529.

  • Maaß S, Bartel J, Mücke PA, Schlüter R, Sura T, Zaschke-Kriesche J, Smits SHJ, Becher D. Proteomic adaptation of Clostridioides difficile to treatment with the antimicrobial peptide nisin.Cells. 2021.

  • Prajapati B, Bernal-Cabas M, López-Álvarez M, Schaffer M, Bartel J, Rath H, Steil L, Becher D, Völker U, Mäder U, van Dijl JM. Double trouble: Bacillus depends on a functional Tat machinery to avoid severe oxidative stress and starvation upon entry into a NaCl-depleted environment. Biochim Biophys Acta Mol Cell Res. 2021 Feb. PMID: 33245978.

  • Hinzke T, Kleiner M, Meister M, Schlüter R, Hentschker C, Pané-Farré J, Hildebrandt P, Felbeck H, Sievert SM, Bonn F, Völker U, Becher D, Schweder T, Markert S. Bacterial symbiont subpopulations have different roles in a deep-sea symbiosis. Elife. 2021 Jan 6. PMID: 33404502.

  • Wencker FDR, Marincola G, Schoenfelder SMK, Maaß S,Becher D, Ziebuhr W. Another layer of complexity in Staphylococcus aureus methionine biosynthesis control: unusual RNase III-driven T-box riboswitch cleavage determines met operon mRNA stability and decay.  Nucleic Acids Res. 2021 Feb 26. PMID: 33450025.

  • Welle M, Pedersen JT, Ravnsborg T, Hayashi M, Maaß S, Becher D, Jensen ON, Stöhr C, Palmgren M. A conserved, buried cysteine near the P-site is accessible to cysteine modifications and increases ROS stability in the P-type plasma membrane H+-ATPase. Biochem J. 2021 Feb 12. PMID: 33427868.

  • Vidal-Melgosa S, Sichert A, Francis TB, Bartosik D, Niggemann J, Wichels A, Willats WGT, Fuchs BM, Teeling H, Becher D, Schweder T, Amann R, Hehemann JH.  Diatom fucan polysaccharide precipitates carbon during algal blooms.  Nat Commun. 2021 Feb 19. PMID: 33608542.

  • Melior H, Li S, Stötzel M, Maaß S, Schütz R, Azarderakhsh S, Shevkoplias A, Barth-Weber S, Baumgardt K, Ziebuhr J, Förstner KU, Chervontseva Z, Becher D, Evguenieva-Hackenberg E. Reprograming of sRNA target specificity by the leader peptide peTrpL in response to antibiotic exposure. Nucleic Acids Res. 2021 Feb 22. PMID: 33619526.

  • Mücke PA, Ostrzinski A, Hammerschmidt S, Maaß S, Becher D. Proteomic Adaptation of Streptococcus pneumoniaeto the Antimicrobial Peptide Human Beta Defensin 3 (hBD3) in Comparison to Other Cell Surface Stresses. Microorganisms. 2020. PMID: 33143252

  • Leonard M, Kühn A, Harting R, Maurus I, Nagel A, Starke J, Kusch H, Valerius O, Feussner K, Feussner I, Kaever A, Landesfeind M, Morgenstern B,Becher D, Hecker M, Braus-Stromeyer SA, Kronstad JW, Braus GH. Verticillium longisporum elicits media-dependent secretome responses With capacity to distinguish between plant-related environments. Front Microbiol. 2020. PMID: 32849460.

  • Bartel J, Varadarajan AR, Sura T, Ahrens CH, Maaß S,Becher D. Optimized proteomics workflow for the detection of small proteins. J Proteome Res. 2020. PMID: 32812434.

  • Lemay ML, Maaß S, Otto A, Hamel J, Plante PL, Rousseau GM, Tremblay DM, Shi R, Corbeil J, Gagné SM, Becher D, Moineau S. A lactococcal phage protein promotes viral propagation and alters the host proteomic response during infection. Viruses. 2020. PMID: 32722163.

  • Zhao X, Chlebowicz-Flissikowska MA, Wang M, Vera Murguia E, de Jong A, Becher D, Maaß S, Buist G, van Dijl JM. Exoproteomic profiling uncovers critical determinants for virulence of livestock-associated and human-originated Staphylococcus aureus ST398 strains. Virulence. 2020. PMID: 32726182.

  • Voichek M, Maaß S, Kroniger T, Becher D, Sorek R. Peptide-based quorum sensing systems in Paenibacillus polymyxa. Life Sci Alliance. 2020.  PMID: 32764104.

  • Ünsaldı E, Kurt-Kızıldoğan A, Özcan S, Becher D, Voigt B, Aktaş C, Özcengiz G. Proteomic analysis of a hom-disrupted, Cephamycin C overproducing Streptomyces clavuligerus. Protein Pept Lett. 2020. PMID: 32707026.

  • Melior H, Maaß S, Li S, Förstner KU, Azarderakhsh S, Varadarajan AR, Stötzel M, Elhossary M, Barth-Weber S, Ahrens CH, Becher D, Evguenieva-Hackenberg E. The leader peptide peTrpL forms antibiotic-containing ribonucleoprotein complexes for posttranscriptional regulation of multiresistance genes. mBio. 2020. PMID: 32546623.

  • Dreisbach A, Wang M, van der Kooi-Pol MM, Reilman E, Koedijk DGAM, Mars RAT, Duipmans J, Jonkman M, Benschop JJ, Bonarius HPJ, Groen H, Hecker M, Otto A, Bäsell K, Bernhardt J, Back JW, Becher D, Buist G, van Dijl JM.  Tryptic shaving of Staphylococcus aureus unveils immunodominant epitopes on the bacterial cell surface. J Proteome Res. 2020. PMID: 32529827.

  • Lilge L, Reder A, Tippmann F, Morgenroth F, Grohmann J, Becher D, Riedel K, Völker U, Hecker M, Gerth U. The Involvement of the McsB Arginine Kinase in Clp-Dependent Degradation of the MgsR Regulator in Bacillus subtilis. Front Microbiol. 2020. PMID: 32477307.

  • Sichert A, Corzett CH, Schechter MS, Unfried F, Markert S, Becher D, Fernandez-Guerra A, Liebeke M, Schweder T, Polz MF, Hehemann JH. Verrucomicrobia use hundreds of enzymes to digest the algal polysaccharide fucoidan. Nat Microbiol. 2020. PMID: 32451471.

  • Okay S, Yildirim V, Büttner K, Becher D, Özcengiz G. Dynamic proteomic analysis of Phanerochaete chrysosporium under copper stress. Ecotoxicol Environ Saf. 2020. PMID: 32388186.

  • Bernal-Cabas M, Miethke M, Antelo-Varela M, Aguilar Suárez R, Neef J, Schön L, Gabarrini G, Otto A,Becher D, Wolf D, van Dijl JM. Functional association of the stress-responsive LiaH protein and the minimal TatAyCy protein translocase in Bacillus subtilis. Biochim Biophys Acta Mol Cell Res. 2020.  PMID: 32302670.

  • Antelo-Varela M, Aguilar Suárez R, Bartel J, Bernal-Cabas M, Stobernack T, Sura T, van Dijl JM, Maaß S, Becher D.  Membrane Modulation of Super-Secreting "midiBacillus" Expressing the Major Staphylococcus aureus Antigen - A Mass-Spectrometry-Based Absolute Quantification Approach.  Front Bioeng Biotechnol. 2020. PMID: 32185169.

  • Mücke PA, Maaß S, Kohler TP, Hammerschmidt S, Becher D. Proteomic Adaptation of Streptococcus pneumoniae to the Human Antimicrobial Peptide LL-37. Microorganisms. 2020.  PMID: 32183275.

  • Giljan G, Kamennaya NA, Otto A, Becher D, Ellrott A, Meyer V, Murton BJ, Fuchs BM, Amann RI, Zubkov MV. Bacterioplankton reveal years-long retention of Atlantic deep-ocean water by the Tropic Seamount.  Sci Rep. 2020. PMID:32170218.

  • Hentschker C, Maaß S, Junker S, Hecker M, Hammerschmidt S, Otto A, Becher D. Comprehensive spectral library from the pathogenic bacterium Streptococcus pneumoniae with focus on phosphoproteins.  J Proteome Res. 2020. PMID:32154730.

  • Hirschfeld C, Gómez-Mejia A, Bartel J, Hentschker C, Rohde M, Maaß S, Hammerschmidt S, Becher D. Proteomic Investigation Uncovers Potential Targets and Target Sites of Pneumococcal Serine-Threonine Kinase StkP and Phosphatase PhpP. Front Microbiol. 2020. PMID:32117081.

  • Karyolaimos A, Dolata KM, Antelo-Varela M, Mestre Borras A, Elfageih R, Sievers S, Becher D, Riedel K, de Gier JW. Escherichia coli Can Adapt Its Protein Translocation Machinery for Enhanced Periplasmic Recombinant Protein Production. Front Bioeng Biotechnol. 2020. PMID:32064253.

  • Ponnudurai R, Heiden SE, Sayavedra L, Hinzke T, Kleiner M, Hentschker C, Felbeck H, Sievert SM, Schlüter R, Becher D, Schweder T, Markert S. Comparative proteomics of related symbiotic mussel species reveals high variability of host-symbiont interactions. ISME J. 2020. PMID:31680119.

  • Henriques G, McGovern S, Neef J, Antelo-Varela M, Götz F, Otto A, Becher D, van Dijl JM, Jules M, Delumeau O. SppI Forms a Membrane Protein Complex with SppA and Inhibits Its Protease Activity in Bacillus subtilis. mSphere. 2020. 5(5):e00724-20

  • Venturini E, Svensson SL, Maaß S, Gelhausen R, Eggenhofer F, Li L, Cain AK, Parkhill J, Becher D, Backofen R, Barquist L, Sharma CM, Westermann AJ, Vogel J. A global data-driven census of Salmonella small proteins and their potential functions in bacterial virulence. microLife. 2020. Online ahead of print

  • Chi BK, Huyen NTT, Loi VV, Gruhlke MCH, Schaffer M, Mäder U, Maaß S, Becher D, Bernhardt J, Arbach M, Hamilton CJ, Slusarenko AJ, Antelmann H. The Disulfide Stress Response and Protein S-thioallylation Caused by Allicin and Diallyl Polysulfanes in Bacillus subtilis as Revealed by Transcriptomics and Proteomics. Antioxidants (Basel). 2019. pii: E605. PMID: 31795512.
  • Hickl O, Heintz-Buschart A, Trautwein-Schult A, Hercog R, Bork P, Wilmes P, Becher D. Sample Preservation and Storage Significantly Impact Taxonomic and Functional Profiles in Metaproteomics Studies of the Human Gut Microbiome. Microorganisms. 2019. PMID: 31546776.
  • Quesada-Ganuza A, Antelo-Varela M, Mouritzen JC, Bartel J, Becher D, Gjermansen M, Hallin PF, Appel KF, Kilstrup M, Rasmussen MD, Nielsen AK. Identification and optimization of PrsA in Bacillus subtilis for improved yield of amylase. Microb Cell Fact. 2019. PMID: 31530286.
  • Antelo-Varela M, Bartel J, Quesada-Ganuza A, Appel K, Bernal-Cabas M, Sura T, Otto A, Rasmussen M, van Dijl JM, Nielsen A, Maaß S, Becher D. Ariadne's Thread in the Analytical Labyrinth of Membrane Proteins: Integration of Targeted and Shotgun Proteomics for Global Absolute Quantification of Membrane Proteins. Anal Chem. 2019. PMID: 31424929.
  • Fischer T, Schorb M, Reintjes G, Kolovou A, Santarella-Mellwig R, Markert S, Rhiel E, Littmann S, Becher D, Schweder T, Harder J. Biopearling of interconnected outer membrane vesicle chains by a marine flavobacterium.Appl Environ Microbiol. 2019. pii: AEM.00829-19. PMID: 31324630.
  • Krüger K, Chafee M, Ben Francis T, Glavina Del Rio T,Becher D, Schweder T, Amann RI, Teeling H. In marine Bacteroidetes the bulk of glycan degradation during algae blooms is mediated by few clades using a restricted set of genes. ISME J. 2019. PMID: 31316134.
  • Reisky L, Préchoux A, Zühlke MK, Bäumgen M, Robb CS, Gerlach N, Roret T, Stanetty C, Larocque R, Michel G, Song T, Markert S, Unfried F, Mihovilovic MD, Trautwein-Schult A, Becher D, Schweder T, Bornscheuer UT, Hehemann JH. A marine bacterial enzymatic cascade degrades the algal polysaccharide ulvan. Nat Chem Biol. 2019. PMID: 31285597.
  • Moeller FU, Webster NS, Herbold CW, Behnam F, Domman D, Albertsen M, Mooshammer M, Markert S, Turaev D, Becher D, Rattei T, Schweder T, Richter A, Watzka M, Nielsen PH, Wagner M. Characterization of a thaumarchaeal symbiont that drives incomplete nitrification in the tropical sponge Ianthella basta. Environ Microbiol. 2019. PMID: 31271506.
  • Herlemann DPR, Markert S, Meeske C, Andersson AF, de Bruijn I, Hentschker C, Unfried F, Becher D, Jürgens K, Schweder T. Individual physiological adaptations enable selected bacterial taxa to prevail during long-term incubations. Appl Environ Microbiol. 2019. pii: AEM.00825-19. PMID: 31152013.
  • Zhao X, Palma L, Stobernack T, Glasner C, de Jong A, Utari P, Setroikromo R, Quax WJ, Otto A, Becher D, Buist G, van Dijl JM. Exoproteome heterogeneity among closely related Staphylococcus aureus t437 isolates and possible implications for virulence. J Proteome Res. 2019. PMID: 31119940.
  • Quintieri L, Zühlke D, Fanelli F, Caputo L, Liuzzi VC, Logrieco AF, Hirschfeld C, Becher D, Riedel K. Proteomic analysis of the food spoiler Pseudomonas fluorescens ITEM 17298 reveals the antibiofilm activity of the pepsin-digested bovine lactoferrin. Food  Microbiol. 2019. 82:177-193. PMID: 31027772.
  • Graf AC, Leonard A, Schäuble M, Rieckmann LM, Hoyer J, Maaß S, Lalk M, Becher D, Pané-Farré J, Riedel K. Virulence factors produced by Staphylococcus aureus biofilms have a moonlighting function contributing to biofilm integrity. Mol Cell Proteomics. 2019. pii: mcp.RA118.001120. PMID: 30850421.
  • Claus H, Hubert K, Becher D, Otto A, Pawlik MC, Lappann I, Strobel L, Vogel U, Johswich K. A homopolymeric adenosine tract in the promoter region of nspA influences factor H-mediated serum resistance in Neisseria meningitidis. Sci Rep. 2019. 9(1):2736. PMID: 30804422.
  • Maaß S, Moog G, Becher D. Subcellular protein fractionation in Legionella pneumophila and preparation of the derived sub-proteomes for analysis by mass spectrometry. Methods Mol Biol. 2019.1921:445-464. PMID: 30694509.
  • Lemay ML, Otto A, Maaß S, Plate K, Becher D, Moineau S. Investigating Lactococcus lactis MG1363 response to phage p2 infection at the proteome level. Mol Cell Proteomics. 2019. pii: mcp.RA118.001135. PMID: 30679258.
  • Misra R, Menon D, Arora G, Virmani R, Gaur M, Naz S, Jaisinghani N, Bhaduri A, Bothra A, Maji A, Singhal A, Karwal P, Hentschker C, Becher D, Rao V, Nandicoori VK, Gandotra S, Singh Y. Tuning the Mycobacterium tuberculosis alternative sigma factor SigF through the multidomain regulator Rv1364c and osmosensory kinase protein kinase D. J Bacteriol. 2019. 201(7). pii: e00725-18. PMID: 30642988.
  • Sokolov EP, Markert S, Hinzke T, Hirschfeld C, Becher D, Ponsuksili S, Sokolova IM. Effects of hypoxia-reoxygenation stress on mitochondrial proteome and bioenergetics of the hypoxia-tolerant marine bivalve Crassostrea gigas.J Proteomics. 2019. 194:99-111. PMID: 30550986.
  • Götz F, Pjevac P, Markert S, McNichol J, Becher D, Schweder T, Mussmann M, Sievert SM. Transcriptomic and proteomic insight into the mechanism of cyclooctasulfur- versus thiosulfate-oxidation by the chemolithoautotroph Sulfurimonas denitrificans. Environ Microbiol. 2019. 21(1):244-258. PMID: 30362214.
  • Junker S, Maaß S, Otto A, Hecker M, Becher D. Toward the Quantitative Characterization of Arginine Phosphorylations in Staphylococcus aureus.J Proteome Res. 2019. 18(1):265-279. PMID: 30358407
  • Koch H, Dürwald A, Schweder T, Noriega-Ortega B, Vidal-Melgosa S, Hehemann JH, Dittmar T, Freese HM, Becher D, Simon M, Wietz M. Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides. ISME J. 2019. 13(1):92-103. PMID: 30116038.
  • Kappelmann L, Krüger K, Hehemann JH, Harder J, Markert S, Unfried F, Becher D, Shapiro N, Schweder T, Amann RI, Teeling H. Polysaccharide utilization loci of North Sea Flavobacteriia as basis for using SusC/D-protein expression for predicting major phytoplankton glycans. ISME J. 2019. 13(1):76-91. PMID: 30111868.


  • Unfried F, Becker S, Robb CS, Hehemann JH, Markert S, Heiden SE, Hinzke T, Becher D, Reintjes G, Krüger K, Avcı B, Kappelmann L, Hahnke RL, Fischer T, Harder J, Teeling H, Fuchs B, Barbeyron T, Amann RI, Schweder T. Adaptive mechanisms that provide competitive advantages to marine bacteroidetes during microalgal blooms. ISME J. 2018. 12(12):2894-2906. PMID: 30061707.
  • Kroniger T, Otto A, Becher D. Proteomic analysis of bacterial (outer) membrane vesicles: progress and clinical potential. Expert Rev Proteomics. 2018. 15(8):623-626. Editorial. PMID: 30068219.
  • Nepal S, Bonn F, Grasso S, Stobernack T, de Jong A, Zhou K, Wedema R, Rosema S, Becher D, Otto A, Rossen JW, van Dijl JM, Bathoorn E. An ancient family of mobile genomic islands introducing cephalosporinase and carbapenemase genes in Enterobacteriaceae. Virulence. 2018;9(1):1377-1389. PMID: 30101693.
  • Hofmann JD, Otto A, Berges M, Biedendieck R, Michel AM, Becher D, Jahn D, Neumann-Schaal M. Metabolic reprogramming of Clostridioides difficile during the stationary phase with the induction of toxin production. Front Microbiol. 2018. 9:1970. PMID: 30186274.
  • Chen J, Robb CS, Unfried F, Kappelmann L, Markert S, Song T, Harder J, Avcı B, Becher D, Xie P, Amann RI, Hehemann JH, Schweder T, Teeling H. Alpha- and beta-mannan utilization by marine Bacteroidetes. Environ Microbiol. 2018. 20(11):4127-4140. PMID: 30246424.
  • Junker S, Maaß S, Otto A, Hecker M, Becher D. Toward the quantitative characterization of arginine phosphorylations in Staphylococcus aureus. J Proteome Res. 2018. [Epub ahead of print] PMID: 30358407.
  • Stobernack T, du Teil Espina M, Mulder LM, Palma Medina LM, Piebenga DR, Gabarrini G, Zhao X, Janssen KMJ, Hulzebos J, Brouwer E, Sura T, Becher D, van Winkelhoff AJ, Götz F, Otto A, Westra J, van Dijl JM. A secreted bacterial peptidylarginine deiminase can neutralize human innate immune defenses. MBio. 2018. 9(5). pii: e01704-18. PMID: 30377277.
  • Trautwein-Schult A, Maaß S, Plate K, Otto A, Becher D. A metabolic labeling strategy for relative protein quantification in Clostridioides difficile. Front Microbiol. 2018. 9:2371. PMID: 30386308.
  • Maaß S, Otto A, Albrecht D, Riedel K, Trautwein-Schult A, Becher D. Proteomic signatures of Clostridium difficile stressed with Metronidazole, Vancomycin, or Fidaxomicin. Cells. 2018. 7(11). pii: E213. PMID: 30445773.
  • Sokolov EP, Markert S, Hinzke T, Hirschfeld C, Becher D, Ponsuksili S, Sokolova IM. Effects of hypoxia-reoxygenation stress on mitochondrial proteome and bioenergetics of the hypoxia-tolerant marine bivalve Crassostrea gigas. J Proteomics. 2018. pii: S1874-3919(18)30429-9. PMID: 30550986.
  • Shulman A, Yair Y, Biran D, Sura T, Otto A, Gophna U, Becher D, Hecker M, Ron EZ. The Escherichia coli type III secretion system 2 has a global effect on cell surface. MBio. 2018. 9(4). PMID: 29970469.
  • Pjevac P, Meier DV, Markert S, Hentschker C, Schweder T, Becher D, Gruber-Vodicka HR, Richter M, Bach W, Amann R, Meyerdierks A. Metaproteogenomic profiling of microbial communities colonizing actively venting hydrothermal chimneys. Front Microbiol. 2018. 9:680. PMID: 29696004.
  • Berges M, Michel AM, Lassek C, Nuss AM, Beckstette M, Dersch P, Riedel K, Sievers S, Becher D, Otto A, Maass S, Rohde M, Eckweiler D, Borrero-de Acuna JM, Jahn M, Neumann-Schaal M, Jahn D. Iron regulation in Clostridioides difficile. Front Microbiol. 2018. 9:3183.
  • Hoyer J, Bartel J, Gómez-Mejia A, Rohde M, Hirschfeld C, Heß N, Sura T, Maaß S, Hammerschmidt S, Becher D. Proteomic response of Streptococcus pneumoniae to iron limitation. Int J Med Microbiol. 2018. [Epub ahead of print]. PMID: 29496408.
  • Müller A, Grein F, Otto A, Gries K, Orlov D, Zarubaev V, Girard M, Sher X, Shamova O, Roemer T, François P, Becher D, Schneider T,Sahl HG, Differential daptomycin resistance development in Staphyloccocus aureus strains with active and mutated gra regulatory systems. Int J Med Microbiol. 2018. 308(3):335-348. PMID: 29429584.
  • Treffon J, Block D, Moche M, Reiß S, Fuchs S, Engelmann S, Becher D, Langhanki L, Mellmann A, Peters G, Kahl BC. Adaptation of Staphylococcus aureus to the airways of cystic fibrosis patients by the up-regulation of superoxide dismutase M and iron-scavenging proteins. J Infect Dis. 2018. [Epub ahead of print]. PMID: 29325044.
  • García-Pérez AN, de Jong A, Junker S, Becher D, Chlebowicz MA, Duipmans JC, Jonkman MF, van Dijl JM. From the wound to the bench: exoproteome interplay between wound-colonizing Staphylococcus aureus strains and co-existing bacteria. Virulence. 2018. 9(1):363-378. PMID: 29233035.
  • Handtke S, Albrecht D, Otto A, Becher D, Hecker M and Voigt B. The proteomic response of B. pumilus cells to glucose starvation. Proteomics. 2018. 18(1). PMID: 29193752.
  • Junker S, Maaß S, Otto A, Michalik S, Morgenroth F, Gerth U, Hecker M, Becher D. Spectral library based analysis of arginine phosphorylations in Staphylococcus aureus. Mol Cell Proteomics. 2018. 17(2) 335-348. PMID: 29183913.
  • Raschdorf O, Bonn F, Zeytuni N, Zarivach R, Becher D, Schüler D. A quantitative assessment of the membrane-integral sub-proteome of a bacterial magnetic organelle. J Proteomics. 2018. 172:89-99. PMID: 29054541.
  • Hentschker C, Dewald C, Otto A, Büttner K, Hecker M, Becher D. Global quantification of phosphoproteins combining metabolic labeling and gel-based proteomics in B. pumilus. ELECTROPHORESIS. 2017. 39(2):334-343. PMID: 28944503.
  • García-Pérez AN, de Jong A, Junker S, Becher D, Chlebowicz MA, Duipmans JC, Jonkman MF, van Dijl JM. From the wound to the bench: exoproteome interplay between wound-colonizing Staphylococcus aureus strains and co-existing bacteria. Virulence. 2017. [Epub ahead of print]. PMID: 29233035.

  • Junker S, Maaß S, Otto A, Michalik S, Morgenroth F, Gerth U, Hecker M, Becher D. Spectral library based analysis of arginine phosphorylations in Staphylococcus aureusMol Cell Proteomics. 2018 17:335-348.  PMID: 29183913.

  • Ünsaldı E, Kurt-Kızıldoğan A, Voigt B, Becher D, Özcengiz G. Proteome-wide alterations in an industrial clavulanic acid producing strain of Streptomyces clavuligerusSynth and Syst Biotechnol. 2017. 2(1):39-48. PMID: 29062960.

  • Otto A, Maaß S, Bonn F, Büttner K, Becher D. An easy and fast protocol for affinity bead-based protein enrichment and storage of proteome samples. Methods Enzymol. 2017. 585:1-13. PMID: 28109424.

  • Michalik S, Depke M, Murr A, Gesell Salazar M, Kusebauch U, Sun Z, Meyer TC, Surmann K, Pförtner H, Hildebrandt P, Weiss S, Palma Medina LM, Gutjahr M, Hammer E, Becher D, Pribyl T, Hammerschmidt S, Deutsch EW, Bader SL, Hecker M, Moritz RL, Mäder U, Völker U, Schmidt F. A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions. Sci Rep. 2017. 7(1):9718. PMID: 28887440.

  • Abendroth U, Adlung N, Otto A, Grüneisen B, Becher D, Bonas U. Identification of new protein-coding genes with a potential role in the virulence of the plant pathogen Xanthomonas euvesicatoria. BMC Genomics. 2017. 18:625. PMID: 28814272.

  • Hillion M, Imber M, Pedre B, Bernhardt J, Saleh M, Loi VV, Maaß S, Becher D, Astolfi Rosado L, Adrian L, Weise C, Hell R, Wirtz M, Messens J, Antelmann H. The glyceraldehyde-3-phosphate dehydrogenase GapDH of Corynebacterium diphtheriae is redox-controlled by protein S-mycothiolation under oxidative stress. Sci Rep. 2017. 7:5020. PMID: 28694441.

  • Arora G, Sajid A, Virmani R, Singhal A, Kumar CMS, Dhasmana N, Khanna T, Maji A, Misra R, Molle V, Becher D, Gerth U, Mande SC, Singh Y. Ser/Thr protein kinase PrkC-mediated regulation of GroEL is critical for biofilm formation in Bacillus anthracis. NPJ Biofilms Microbiomes. 2017. 3:7. PMID: 28649408.

  • Bonn F, Maaß S, Becher D. Sample preparation for mass spectrometry-based absolute protein quantification in antibiotic stress research. Methods Mol Biol. 2017. 1520:281-289. PMID: 27873259.

  • Hehemann JH, Truong LV, Unfried F, Welsch N, Kabisch J, Heiden SE, Junker S, Becher D, Thürmer A, Daniel R, Amann R, Schweder T. Aquatic adaptation of a laterally acquired pectin degradation pathway in marine gammaproteobacteria. Environ Microbiol. 2017. 19(6):2320-2333. PMID: 28276126.

  • Ponnudurai R, Kleiner M, Sayavedra L, Petersen JM, Moche M, Otto A, Becher D, Takeuchi T, Satoh N, Dubilier N, Schweder T, Markert S. Metabolic and physiological interdependencies in the Bathymodiolus  azoricus symbiosis. ISME J. 2017. 11(2):463-477. PMID: 27801908.

  • Schmölders J, Manske C, Otto A, Hoffmann C, Steiner B, Welin A, Becher D, Hilbi H. Comparative proteomics of purified pathogen vacuoles correlates intracellular replication of Legionella pneumophila with the small GTPase Ras-related protein 1 (Rap1). Mol Cell Proteomics. 2017. 16(4):622-641. PMID: 28183814.

  • Giovannelli D, Sievert SM, Hügler M, Markert S, Becher D, Schweder T, Vetriani C. Insight into the evolution of microbial metabolism from the deep-branching bacterium, Thermovibrio ammonificans. Elife. 2017. 6:e18990. PMID: 28436819.

  • Hillion M, Bernhardt J, Busche T, Rossius M, Maaß S, Becher D, Rawat M, Wirtz M, Hell R, Rückert C, Kalinowski J, Antelmann H. Monitoring global protein thiol-oxidation and protein S-mycothiolation in Mycobacterium smegmatis under hypochlorite stress. Sci Rep. 2017. 7(1):1195. PMID: 28446771.

  • Handtke S, Albrecht D, Zühlke D, Otto A, Becher D, Schweder T, Riedel K, Hecker M, Voigt B. Bacillus pumilus KatX2 confers enhanced hydrogen peroxide resistance to a Bacillus subtilis PkatA::katX2 mutant strain. Microb Cell Fact. 2017. 16(1):72. PMID: 28446175.

  • Mekonnen SA, Palma Medina LM, Glasner C, Tsompanidou E, de Jong A, Grasso S, Schaffer M, Mäder U, Larsen AR, Gumpert H, Westh H, Völker U, Otto A, Becher D, van Dijl JM. Signatures of cytoplasmic proteins in the exoproteome distinguish community- and hospital-associated methicillin-resistant Staphylococcus aureus USA300 lineages. Virulence. 2017. 8(6):891-907. PMID: 28475476.

  • Eymann C, Lassek C, Wegner U, Bernhardt J, Fritsch OA, Fuchs S, Otto A, Albrecht D, Schiefelbein U, Cernava T, Aschenbrenner I, Berg G, Grube M, Riedel K. Symbiotic interplay of fungi, algae, and bacteria within the lung lichen Lobaria pulmonaria L. Hoffm. as assessed by state-of-the-art metaproteomics. J Proteome Res. 2017. 16(6):2160-2173. PMID: 28290203.
  • Ponnudurai R, Kleiner M, Sayavedra L, Petersen JM, Moche M, Otto A, Becher D, Takeuchi T, Satoh N, Dubilier N, Schweder T, Markert S,. Metabolic and physiological interdependencies in the Bathymodiolus azoricus symbiosis. ISME J.2016 Nov 1. PMID: 27801908.
  • König S, Gros O, Heiden SE, Hinzke T, Thürmer A, Poehlein A, Meyer S, Vatin M, Mbéguié-A-Mbéguié D, Tocny J, Ponnudurai R, Daniel R, Becher D, Schweder T, Markert S,. Nitrogen fixation in a chemoautotrophic lucinid symbiosis. Nat Microbiol.2016 Oct 24;2:16193. PMID: 27775698.

  • Stobernack T, Glasner C, Junker S, Gabarrini G, de Smit M, de Jong A, Otto A, Becher D, van Winkelhoff AJ, van Dijl JM. The Extracellular Proteome and Citrullinome of the Oral Pathogen Porphyromonas gingivalis. J Proteome Res.2016 Oct 7. PMID: 27712078.

  • Lappann M, Otto A, Brauer M, Becher D, Vogel U, Johswich K. Impact of moderate temperature changes on Neisseria meningitidis adhesive phenotypes and proteome. Infect Immun.2016 Sep 26. PMID: 27672084.

  • Smirnov A, Förstner KU, Holmqvist E, Otto A, Günster R, Becher D, Reinhardt R, Vogel J. Grad-seq guides the discovery of ProQ as a major small RNA-binding protein. Proc Natl Acad Sci U S A.2016 Oct 11;113(41):11591-11596. PMID: 27671629.

  • Otto A, Biran D, Sura T, Becher D, Ron EZ. Proteomics of septicemic Escherichia coli. Proteomics Clin Appl.2016 Oct;10(9-10):1020-1024. PMID: 27604157.

  • Otto A, Maaß S, Lassek C, Becher D, Hecker M, Riedel K, Sievers S. The protein inventory of Clostridium difficile grown in complex and minimal medium. Proteomics Clin Appl.2016 Oct;10(9-10):1068-1072. PMID: 27511832.

  • Zühlke D, Dörries K, Bernhardt J, Maaß S, Muntel J, Liebscher V, Pané-Farré J, Riedel K, Lalk M, Völker U, Engelmann S, Becher D, Fuchs S, Hecker M. Costs of life – Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis. Sci Rep.2016 Jun 27;6:28172. PMID: 27344979.
  • López-Mondéjar R, Zühlke D, Větrovský T, Becher D, Riedel K, Baldrian P. Decoding the complete arsenal for cellulose and hemicellulose deconstruction in the highly efficient cellulose decomposer Paenibacillus O199. Biotechnol Biofuels. 2016. 9:104. PMID: 27186238

  • Taha MK, Claus H, Lappann M, Veyrier FJ, Otto A, Becher D, Deghmane AE, Frosch M, Hellenbrand W, Hong E, Parent du Châtelet I, Prior K, Harmsen D, Vogel U. Evolutionary Events Associated with an Outbreak of Meningococcal Disease in Men Who Have Sex with Men. PLoS One. 2016. 11(5):e0154047. PMID: 27167067

  • López-Mondéjar R, Zühlke D, Becher D, Riedel K, Baldrian P. Cellulose and hemicellulose decomposition by forest soil bacteria proceeds by the action of structurally variable enzymatic systems. Sci Rep. 2016. 29;6:25279. PMID: 27125755

  • Herweg JA, Pons V, Becher D, Hecker M, Krohne G, Barbier J, Berger H, Rudel T, Mehlitz A. Proteomic analysis of the Simkania-containing vacuole: the central role of retrograde transport. Mol Microbiol. 2016. 99(1):151-71. PMID: 26374382

  • Aurass P, Gerlach T, Becher D, Voigt B, Karste S, Bernhardt J, Riedel K, Hecker M, Flieger A. Life stage-specific proteomes of Legionella pneumophila reveal a highly differential abundance of virulence-associated Dot/Icm effectors. Mol Cell Proteomics. 2016. 15(1):177-200. PMID: 26545400

  • Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Pühler A. Comparative proteome analysis of Actinoplanes sp. SE50/110 grown with maltose or glucose shows minor differences for acarbose biosynthesis proteins but major differences for saccharide transporters. J Proteomics. 2016. 131:140-8. PMID: 26597626

  • Dietz S, Lassek C, Mack SL, Ritzmann M, Stadler J, Becher D, Hoelzle K, Riedel K, Hoelzle LE. Updating the proteome of the uncultivable hemotrophic Mycoplasma suis in experimentally infected pigs. Proteomics. 2016. 16(4):609-13. PMID: 26678042

  • Maaß S, Becher D. Methods and applications of absolute protein quantification in Microbial Systems. J Proteomics. 2016. 136:222-33. PMID: 26825536

  • Bonn F, Pané-Farré J, Schlüter R, Schaffer M, Fuchs S, Bernhardt J, Riedel K, Otto A, Völker U, Dijl JM, Hecker M, Mäder U, Becher D. Global analysis of the impact of linezolid onto virulence factor production in S. aureus USA300. Int J Med Microbiol. 2016. 306(3):131-40. PMID: 26996810

Goelzer A, Muntel J, Chubukov V, Jules M, Prestel E, Nölker R, Mariadassou M, Aymerich S, Hecker M, Noirot P, Becher D, Fromion V. Quantitative prediction of genome-wide resource allocation in bacteria. Metab Eng. 2015; 32:232-43. PMID: 26498510

Sayavedra L, Kleiner M, Ponnudurai R, Wetzel S, Pelletier E, Barbe V, Satoh N, Shoguchi E, Fink D, Breusing C, Reusch TB, Rosenstiel P, Schilhabel MB, Becher D, Schweder T, Markert S, Dubilier N, Petersen JM. Abundant toxin-related genes in the genomes of beneficial symbionts from deep-sea hydrothermal vent mussels. Elife. 2015; 4. pii: e07966. PMID: 26371554

Xing P, Hahnke RL, Unfried F, Markert S, Huang S, Barbeyron T, Harder J, Becher D, Schweder T, Glöckner FO, Amann RI, Teeling H. Niches of two polysaccharide-degrading Polaribacter isolates from the North Sea during a spring diatom bloom. ISME J. 2015; 9(6):1410-22. PMID: 25478683

Singhal A, Arora G, Virmani R, Kundu P, Khanna T, Sajid A, Misra R, Joshi J, Yadav V, Samanta S, Saini N, Pandey AK, Visweswariah SS, Hentschker C, Becher D, Gerth U, Singh Y. Systematic analysis of mycobacterial acylation reveals first example of acylation-mediated regulation of enzyme activity of a bacterial phosphatase. J Biol Chem. 2015; 290(43):26218-34. PMID: 26350458

Heazlewood JL, Schrimpf SP, Becher D, Riedel K, Tholey A, Bendixen E. Multi-Organism Proteomes (iMOP): Advancing our understanding of human biology. Proteomics. 2015;15(17):2885-94. PMID: 26331910

Theilacker C, Diederich AK, Otto A, Sava IG, Wobser D, Bao Y, Hese K, Broszat M, Henneke P, Becher D, Huebner J. Enterococcus faecalis glycolipids modulate lipoprotein-content of the bacterial cell membrane and host immune response. PLoS One. 2015;10(7):e0132949. PMID: 26172831

Neef J, Milder FJ, Koedijk DG, Klaassens M, Heezius EC, van Strijp JA, Otto A, Becher D, van Dijl JM, Buist G. Versatile vector suite for the extracytoplasmic production and purification of heterologous His-tagged proteins in Lactococcus lactis. Appl Microbiol Biotechnol. 2015; 99(21):9037-48. PMID: 26160391

Moche M, Schlüter R, Bernhardt J, Plate K, Riedel K, Hecker M, Becher D. Time-resolved analysis of cytosolic and surface-associated proteins of Staphylococcus aureus HG001 under planktonic and biofilm conditions. J Proteome Res. 2015;14(9):3804-22. PMID: 26152824

Herweg JA, Hansmeier N, Otto A, Geffken AC, Subbarayal P, Prusty BK, Becher D, Hensel M, Schaible UE, Rudel T, Hilbi H. Purification and proteomics of pathogen-modified vacuoles and membranes. Front Cell Infect Microbiol. 2015; 5:48. Review. PMID: 26082896

Kern B, Jain U, Utsch C, Otto A, Busch B, Jiménez-Soto L, Becher D, Haas R. Characterization of Helicobacter pylori VacA-containing vacuoles (VCVs), VacA intracellular trafficking and interference with calcium signalling in T lymphocytes. Cell Microbiol. 2015. 17(12):1811-32. PMID: 26078003

Stopnisek N, Zühlke D, Carlier A, Barberán A, Fierer N, Becher D, Riedel K, Eberl L and Weisskopf L. Molecular mechanisms underlying the close association between soil Burkholderia and fungi. ISME J. 2015. 10(1):253-64. PMID: 25989372

Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Walter F, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Pühler A. Comprehensive proteome analysis of Actinoplanes sp. SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster. J Proteomics. 2015. 125:1-16. PMID: 25896738

Prasse D, Thomsen J, De Santis R, Muntel J, Becher D, Schmitz RA. First description of small proteins encoded by spRNAs in Methanosarcina mazei strain Gö1. Biochimie. 2015; 117:138-48 PMID: 25890157

Voigt B, Albrecht D, Sievers S, Becher D, Bongaerts J, Evers S, Schweder T, Maurer KH, Hecker M. High resolution proteome maps of Bacillus licheniformis cells growing in minimal medium. Proteomics. 2015;15(15):2629-33. PMID: 25867794

Brack C, Mikolasch A, Schlueter R, Otto A, Becher D, Wegner U, Albrecht D, Riedel K, Schauer F. Antibacterial metabolites and bacteriolytic enzymes produced by Bacillus pumilus during bacteriolysis of Arthrobacter citreus. Mar Biotechnol (NY). 2015; 17(3):290-304. PMID: 25678259

Lassek C, Burghartz M, Chaves-Moreno D, Otto A, Hentschker C, Fuchs S, Bernhardt J, Jauregui R, Neubauer R, Becher D, Pieper DH, Jahn M, Jahn D, Riedel K. A Metaproteomics approach to elucidate host and pathogen protein expression during catheter-associated urinary tract infections (CAUTIs). Mol Cell Proteomics. 2015; 14(4):989-1008. PMID: 25673765

Salzberg LI, Botella E, Hokamp K, Antelmann H, Maaß S, Becher D, Noone D, Devine KM. Genome-Wide Analysis of Phosphorylated PhoP Binding to Chromosomal DNA Reveals Several Novel Features of the PhoPR-Mediated Phosphate Limitation Response in Bacillus subtilis. J Bacteriol. 2015; 197(8):1492-506. PMID: 25666134

AbouElfetouh A, Kuhn ML, Hu LI, Scholle MD, Sorensen DJ, Sahu AK, Becher D, Antelmann H, Mrksich M, Anderson WF, Gibson BW, Schilling B, Wolfe AJ. The E. coli sirtuin CobB shows no preference for enzymatic and nonenzymatic lysine acetylation substrate sites. Microbiologyopen. 2015;4(1):66-83. PMID: 25417765


Handtke S, Volland S, Methling K, Albrecht D, Becher D, Nehls J, Bongaerts J, Maurer KH, Lalk M, Liesegang H, Voigt B, Daniel R, Hecker M. Cell physiology of the biotechnological relevant bacterium Bacillus pumilus-An omics-based approach. J Biotechnol. 2014;192 Pt A:204-14. PMID: 25281541

Kohlmann Y, Pohlmann A, Schwartz E, Zühlke D, Otto A, Albrecht D, Grimmler C, Ehrenreich A, Voigt B, Becher D, Hecker M, Friedrich B, Cramm R. Coping with Anoxia: A Comprehensive Proteomic and Transcriptomic Survey of Denitrification. J Proteome Res. 2014; 13(10):4325-38. PMID: 25198380

Maaβ S, Wachlin G, Bernhardt J, Eymann C, Fromion V, Riedel K, Becher D, Hecker M. Highly Precise Quantification of Protein Molecules per Cell During Stress and Starvation Responses in Bacillus subtilis. Mol Cell Proteomics. 2014;13(9):2260-76. PMID: 24878497.

Huja S, Oren Y, Biran D, Meyer S, Dobrindt U, Bernhard J, Becher D, Hecker M, Sorek R, Ron EZ. Fur is the master regulator of the extraintestinal pathogenic Escherichia coli response to serum. MBio. 2014;5(4). pii: e01460-14. PMID: 25118243.

Bonn F, Bartel J, Büttner K, Hecker M, Otto A, Becher D. Picking vanished proteins from the void: how to collect and ship/share extremely dilute proteins in a reproducible and highly efficient manner. Anal Chem. 2014;86(15):7421-7. PMID: 24987932.

Grube M, Cernava T, Soh J, Fuchs S, Aschenbrenner I, Lassek C, Wegner U, Becher D, Riedel K, Sensen CW, Berg G. Exploring functional contexts of symbiotic sustain within lichen-associated bacteria by comparative omics. ISME J. 2014. doi: 10.1038/ismej.2014.138. PMID: 25072413.

Gaballa A, Chi BK, Roberts AA, Becher D, Hamilton CJ, Antelmann H, Helmann JD. Redox regulation in Bacillus subtilis: The bacilliredoxins BrxA(YphP) and BrxB(YqiW) function in de-bacillithiolation of S-bacillithiolated OhrR and MetE. Antioxid Redox Signal. 2014;21(3):357-67. PMID: 24313874.

Hoffmann C, Finsel I, Otto A, Pfaffinger G, Rothmeier E, Hecker M, Becher D, Hilbi H. Functional analysis of novel Rab GTPases identified in the proteome of purified Legionella-containing vacuoles from macrophages. Cell Microbiol. 2014;16(7):1034-52. PMID: 24373249.

Kabisch A, Otto A, König S, Becher D, Albrecht D, Schüler M, Teeling H, Amann RI, Schweder T. Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes ‘Gramella forsetii‘ KT0803. ISME J. 2014;8(7):1492-502. PubMed PMID: 24522261.

Petasch J, Disch EM, Markert S, Becher D, Schweder T, Hüttel B, Reinhardt R, Harder J. The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen. BMC Microbiol. 2014;14:164. PMID: 24952578.

Wenzel M, Chiriac AI, Otto A, Zweytick D, May C, Schumacher C, Gust R, Albada HB, Penkova M, Krämer U, Erdmann R, Metzler-Nolte N, Straus SK, Bremer E, Becher D, Brötz-Oesterhelt H, Sahl HG, Bandow JE. Small cationic antimicrobial peptides delocalize peripheral membrane proteins. Proc Natl Acad Sci U S A. 2014;111(14):E1409-18. PMID: 24706874.

Muntel J, Fromion V, Goelzer A, Maaβ S, Mäder U, Büttner K, Hecker M, Becher D. Comprehensive absolute quantification of the cytosolic proteome of Bacillus subtilis by data independent, parallel fragmentation in liquid chromatography/mass spectrometry (LC/MS(E)). Mol Cell Proteomics. 2014;13(4):1008-19. PMID: 24696501.

Bäsell K, Otto A, Junker S, Zühlke D, Rappen GM, Schmidt S, Hentschker C, Macek B, Ohlsen K, Hecker M, Becher D. The phosphoproteome and its physiological dynamics in Staphylococcus aureus. Int J Med Microbiol. 2014;304(2):121-32. PMID: 24457182.

Otto A, van Dijl JM, Hecker M, Becher D. The Staphylococcus aureus proteome. Int J Med Microbiol. 2014;304(2):110-20. Review. PMID: 24439828.

Otto A, Becher D, Schmidt F. Quantitative proteomics in the field of microbiology. Proteomics. 2014;14(4-5):547-65. PMID: 24376008.

Pribyl T, Moche M, Dreisbach A, Bijlsma JJ, Saleh M, Abdullah MR, Hecker M, van Dijl JM, Becher D, Hammerschmidt S. Influence of impaired lipoprotein biogenesis on surface and exoproteome of Streptococcus pneumoniae. J Proteome Res. 2014;13(2):650-67. PMID: 24387739.

Chi BK, Busche T, Van Laer K, Bäsell K, Becher D, Clermont L, Seibold GM, Persicke M, Kalinowski J, Messens J, Antelmann H. Protein S-mycothiolation functions as redox-switch and thiol protection mechanism in Corynebacterium glutamicum under hypochlorite stress. Antioxid Redox Signal. 2014;20(4):589-605. PMID: 23886307.

Noone D, Salzberg LI, Botella E, Bäsell K, Becher D, Antelmann H, Devine KM. A highly unstable transcript makes CwlO D,L-endopeptidase expression responsive to growth conditions in Bacillus subtilis. J Bacteriol. 2014;196(2):237-47. PMID: 24163346.

Miranda HV, Antelmann H, Hepowit N, Chavarria NE, Krause DJ, Pritz JR, Bäsell K, Becher D, Humbard MA, Brocchieri L, Maupin-Furlow JA. Archaeal ubiquitin-like SAMP3 is isopeptide-linked to proteins via a UbaA-dependent mechanism. Mol Cell Proteomics. 2014;13(1):220-39. PMID: 24097257.


Hessling B, Bonn F, Otto A, Herbst FA, Rappen GM, Bernhardt J, Hecker M, Becher D. Global proteome analysis of vancomycin stress in Staphylococcus aureus. Int J Med Microbiol. 2013;303(8):624-34. PMID: 24161710.

Greiner R, Pálinkás Z, Bäsell K, Becher D, Antelmann H, Nagy P, Dick TP. Polysulfides link H2S to protein thiol oxidation. Antioxid Redox Signal. 2013;19(15):1749-65. PMID: 23646934.

Lappann M, Otto A, Becher D, Vogel U. Comparative proteome analysis of spontaneous outer membrane vesicles and purified outer membranes of Neisseria meningitidis. J Bacteriol. 2013;195(19):4425-35. PMID: 23893116.

Hessling B, Büttner K, Hecker M, Becher D. Global relative quantification with liquid chromatography-matrix-assisted laser desorption ionization time-of-flight (LC-MALDI-TOF)–cross-validation with LTQ-Orbitrap proves reliability and reveals complementary ionization preferences. Mol Cell Proteomics. 2013;12(10):2911-20. PMID: 23788530.

Becher D, Bernhardt J, Fuchs S, Riedel K. Metaproteomics to unravel major microbial players in leaf litter and soil environments: challenges and perspectives. Proteomics. 2013;13(18-19):2895-909. Review. PMID: 23894095.

Krishnappa L, Dreisbach A, Otto A, Goosens VJ, Cranenburgh RM, Harwood CR, Becher D, van Dijl JM. Extracytoplasmic proteases determining the cleavage and release of secreted proteins, lipoproteins, and membrane proteins in Bacillus subtilis. J Proteome Res. 2013;12(9):4101-10. PMID: 23937099.

Hu LI, Chi BK, Kuhn ML, Filippova EV, Walker-Peddakotla AJ, Bäsell K, Becher D, Anderson WF, Antelmann H, Wolfe AJ. Acetylation of the response regulator RcsB controls transcription from a small RNA promoter. J Bacteriol. 2013;195(18):4174-86. PMID: 23852870.

Musa YR, Bäsell K, Schatschneider S, Vorhölter FJ, Becher D, Niehaus K. Dynamic protein phosphorylation during the growth of Xanthomonas campestris pv. campestris B100 revealed by a gel-based proteomics approach. J Biotechnol. 2013;167(2):111-22. PMID: 23792782.

Chi BK, Roberts AA, Huyen TT, Bäsell K, Becher D, Albrecht D, Hamilton CJ, Antelmann H. S-bacillithiolation protects conserved and essential proteins against hypochlorite stress in firmicutes bacteria. Antioxid Redox Signal. 2013;18(11):1273-95. PMID: 22938038.

Tsompanidou E, Denham EL, Becher D, de Jong A, Buist G, van Oosten M, Manson WL, Back JW, van Dijl JM, Dreisbach A. Distinct roles of phenol-soluble modulins in spreading of Staphylococcus aureus on wet surfaces. Appl Environ Microbiol. 2013;79(3):886-95. PMID: 23183971.

Goosens VJ, Otto A, Glasner C, Monteferrante CC, van der Ploeg R, Hecker M, Becher D, van Dijl JM. Novel twin-arginine translocation pathway-dependent phenotypes of Bacillus subtilis unveiled by quantitative proteomics. J Proteome Res. 2013;12(2):796-807. PMID: 23256564.

Schroeter R, Hoffmann T, Voigt B, Meyer H, Bleisteiner M, Muntel J, Jürgen B, Albrecht D, Becher D, Lalk M, Evers S, Bongaerts J, Maurer KH, Putzer H, Hecker M, Schweder T, Bremer E. Stress responses of the industrial workhorse Bacillus licheniformis to osmotic challenges. PLoS One. 2013;8(11):e80956. PMID: 24348917.

Celik H, Blouzard JC, Voigt B, Becher D, Trotter V, Fierobe HP, Tardif C, Pagès S, de Philip P. A two-component system (XydS/R) controls the expression of genes encoding CBM6-containing proteins in response to straw in Clostridium cellulolyticum. PLoS One. 2013;8(2):e56063. PMID: 23418511.


Sibbald MJ, Yang XM, Tsompanidou E, Qu D, Hecker M, Becher D, Buist G, van Dijl JM. Partially overlapping substrate specificities of staphylococcal group A sortases. Proteomics. 2012;12(19-20):3049-62. PMID: 22930668.

Lima BP, Thanh Huyen TT, Bäsell K, Becher D, Antelmann H, Wolfe AJ. Inhibition of acetyl phosphate-dependent transcription by an acetylatable lysine on RNA polymerase. J Biol Chem. 2012;287(38):32147-60. PMID: 22829598.

Michalik S, Bernhardt J, Otto A, Moche M, Becher D, Meyer H, Lalk M, Schurmann C, Schlüter R, Kock H, Gerth U, Hecker M. Life and death of proteins: a case study of glucose-starved Staphylococcus aureus. Mol Cell Proteomics. 2012;11(9):558-70. PMID: 22556279.

Otto A, Bernhardt J, Hecker M, Becher D. Global relative and absolute quantitation in microbial proteomics. Curr Opin Microbiol. 2012;15(3):364-72. Review. PMID: 22445110.

Voigt B, Hieu CX, Hempel K, Becher D, Schlüter R, Teeling H, Glöckner FO, Amann R, Hecker M, Schweder T. Cell surface proteome of the marine planctomycete Rhodopirellula baltica. Proteomics. 2012;12(11):1781-91. PMID: 22623273.

Kleiner M, Wentrup C, Lott C, Teeling H, Wetzel S, Young J, Chang YJ, Shah M, VerBerkmoes NC, Zarzycki J, Fuchs G, Markert S, Hempel K, Voigt B, Becher D, Liebeke M, Lalk M, Albrecht D, Hecker M, Schweder T, Dubilier N. Metaproteomics of a gutless marine worm and its symbiotic microbial community reveal unusual pathways for carbon and energy use. Proc Natl Acad Sci U S A. 2012;109(19):E1173-82. PMID: 22517752.

Elsholz AK, Turgay K, Michalik S, Hessling B, Gronau K, Oertel D, Mäder U, Bernhardt J, Becher D, Hecker M, Gerth U. Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis. Proc Natl Acad Sci U S A. 2012;109(19):7451-6. PMID: 22517742.

Teeling H, Fuchs BM, Becher D, Klockow C, Gardebrecht A, Bennke CM, Kassabgy M, Huang S, Mann AJ, Waldmann J, Weber M, Klindworth A, Otto A, Lange J, Bernhardt J, Reinsch C, Hecker M, Peplies J, Bockelmann FD, Callies U, Gerdts G, Wichels A, Wiltshire KH, Glöckner FO, Schweder T, Amann R. Substrate-controlled succession of marine bacterioplankton populations induced by a phytoplankton bloom. Science. 2012;336(6081):608-11. PMID: 22556258.

Palm GJ, Khanh Chi B, Waack P, Gronau K, Becher D, Albrecht D, Hinrichs W, Read RJ, Antelmann H. Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR. Nucleic Acids Res. 2012;40(9):4178-92. PMID: 22238377.

Muntel J, Hecker M, Becher D. An exclusion list based label-free proteome quantification approach using an LTQ Orbitrap. Rapid Commun Mass Spectrom. 2012;26(6):701-9. PMID: 22328225.

Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E, Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E, Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P, Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B, Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J, Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P, Noirot P. Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis. Science. 2012;335(6072):1103-6. PMID: 22383849.

Buescher JM, Liebermeister W, Jules M, Uhr M, Muntel J, Botella E, Hessling B, Kleijn RJ, Le Chat L, Lecointe F, Mäder U, Nicolas P, Piersma S, Rügheimer F, Becher D, Bessieres P, Bidnenko E, Denham EL, Dervyn E, Devine KM, Doherty G, Drulhe S, Felicori L, Fogg MJ, Goelzer A, Hansen A, Harwood CR, Hecker M, Hubner S, Hultschig C, Jarmer H, Klipp E, Leduc A, Lewis P, Molina F, Noirot P, Peres S, Pigeonneau N, Pohl S, Rasmussen S, Rinn B, Schaffer M, Schnidder J, Schwikowski B, Van Dijl JM, Veiga P, Walsh S, Wilkinson AJ, Stelling J, Aymerich S, Sauer U. Global network reorganization during dynamic adaptations of Bacillus subtilis metabolism. Science. 2012;335(6072):1099-103. PMID: 22383848.

Heinz E, Williams TA, Nakjang S, Noël CJ, Swan DC, Goldberg AV, Harris SR, Weinmaier T, Markert S, Becher D, Bernhardt J, Dagan T, Hacker C, Lucocq JM, Schweder T, Rattei T, Hall N, Hirt RP, Embley TM. The genome of the obligate intracellular parasite Trachipleistophora hominis: new insights into microsporidian genome dynamics and reductive evolution. PLoS Pathog. 2012;8(10):e1002979. PMID: 23133373.


Chi BK, Gronau K, Mäder U, Hessling B, Becher D, Antelmann H. S-bacillithiolation protects against hypochlorite stress in Bacillus subtilis as revealed by transcriptomics and redox proteomics. Mol Cell Proteomics. 2011;10(11):M111.009506. PMID: 21749987.

Miller M, Dreisbach A, Otto A, Becher D, Bernhardt J, Hecker M, Peppelenbosch MP, van Dijl JM. Mapping of interactions between human macrophages and Staphylococcus aureus reveals an involvement of MAP kinase signaling in the host defense. J Proteome Res. 2011;10(9):4018-32. PMID: 21736355.

Lima BP, Antelmann H, Gronau K, Chi BK, Becher D, Brinsmade SR, Wolfe AJ. Involvement of protein acetylation in glucose-induced transcription of a stress-responsive promoter. Mol Microbiol. 2011;81(5):1190-204. PMID: 21696463.

Schmidl SR, Otto A, Lluch-Senar M, Piñol J, Busse J, Becher D, Stülke J. A trigger enzyme in Mycoplasma pneumoniae: impact of the glycerophosphodiesterase GlpQ on virulence and gene expression. PLoS Pathog. 2011;7(9):e1002263. PMID: 21966272.

Elsholz AK, Hempel K, Michalik S, Gronau K, Becher D, Hecker M, Gerth U. Activity control of the ClpC adaptor McsB in Bacillus subtilis. J Bacteriol. 2011;193(15):3887-93. PMID: 21622759.

Markert S, Gardebrecht A, Felbeck H, Sievert SM, Klose J, Becher D, Albrecht D, Thürmer A, Daniel R, Kleiner M, Hecker M, Schweder T. Status quo in physiological proteomics of the uncultured Riftia pachyptila endosymbiont. Proteomics. 2011;11(15):3106-17. PMID: 21710568.

Delumeau O, Lecointe F, Muntel J, Guillot A, Guédon E, Monnet V, Hecker M, Becher D, Polard P, Noirot P. The dynamic protein partnership of RNA polymerase in Bacillus subtilis. Proteomics. 2011;11(15):2992-3001. PMID: 21710567.

Becher D, Büttner K, Moche M, Hessling B, Hecker M. From the genome sequence to the protein inventory of Bacillus subtilis. Proteomics. 2011;11(15):2971-80. Review. PMID: 21710564.

Dreisbach A, van der Kooi-Pol MM, Otto A, Gronau K, Bonarius HP, Westra H, Groen H, Becher D, Hecker M, van Dijl JM. Surface shaving as a versatile tool to profile global interactions between human serum proteins and the Staphylococcus aureus cell surface. Proteomics. 2011;11(14):2921-30. PMID: 21674804.

Albaum SP, Hahne H, Otto A, Haußmann U, Becher D, Poetsch A, Goesmann A, Nattkemper TW. A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study. Proteome Sci. 2011;9:30. PMID: 21663690.

Kohlmann Y, Pohlmann A, Otto A, Becher D, Cramm R, Lütte S, Schwartz E, Hecker M, Friedrich B. Analyses of soluble and membrane proteomes of Ralstonia eutropha H16 reveal major changes in the protein complement in adaptation to lithoautotrophy. J Proteome Res. 2011;10(6):2767-76. PMID: 21561103.

Eymann C, Schulz S, Gronau K, Becher D, Hecker M, Price CW. In vivo phosphorylation patterns of key stressosome proteins define a second feedback loop that limits activation of Bacillus subtilis σB. Mol Microbiol. 2011;80(3):798-810. PMID: 21362065.

Tefon BE, Maass S, Ozcengiz E, Becher D, Hecker M, Ozcengiz G. A comprehensive analysis of Bordetella pertussis surface proteome and identification of new immunogenic proteins. Vaccine. 2011;29(19):3583-95. PMID: 21397717.

Maass S, Sievers S, Zühlke D, Kuzinski J, Sappa PK, Muntel J, Hessling B, Bernhardt J, Sietmann R, Völker U, Hecker M, Becher D. Efficient, global-scale quantification of absolute protein amounts by integration of targeted mass spectrometry and two-dimensional gel-based proteomics. Anal Chem. 2011;83(7):2677-84. PMID: 21395229.

Hempel K, Herbst FA, Moche M, Hecker M, Becher D. Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions. J Proteome Res. 2011;10(4):1657-66. PMID: 21323324.

Yıldırım V, Ozcan S, Becher D, Büttner K, Hecker M, Ozcengiz G. Characterization of proteome alterations in Phanerochaete chrysosporium in response to lead exposure. Proteome Sci. 2011;9:12. PMID: 21388532.

Elsholz AK, Hempel K, Pöther DC, Becher D, Hecker M, Gerth U. CtsR inactivation during thiol-specific stress in low GC, Gram+ bacteria. Mol Microbiol. 2011;79(3):772-85. PMID: 21208299.

Saller MJ, Otto A, Berrelkamp-Lahpor GA, Becher D, Hecker M, Driessen AJ. Bacillus subtilis YqjG is required for genetic competence development. Proteomics. 2011;11(2):270-82. PMID: 21204254.


Price CE, Otto A, Fusetti F, Becher D, Hecker M, Driessen AJ. Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions. Proteomics. 2010;10(18):3235-47. PMID: 20706981.

Dreisbach A, Hempel K, Buist G, Hecker M, Becher D, van Dijl JM. Profiling the surfacome of Staphylococcus aureus. Proteomics. 2010;10(17):3082-96. PMID: 20662103.

Chi BK, Albrecht D, Gronau K, Becher D, Hecker M, Antelmann H. The redox-sensing regulator YodB senses quinones and diamide via a thiol-disulfide switch in Bacillus subtilis. Proteomics. 2010;10(17):3155-64. PMID: 20652907.

Hyyryläinen HL, Marciniak BC, Dahncke K, Pietiäinen M, Courtin P, Vitikainen M, Seppala R, Otto A, Becher D, Chapot-Chartier MP, Kuipers OP, Kontinen VP. Penicillin-binding protein folding is dependent on the PrsA peptidyl-prolyl cis-trans isomerase in Bacillus subtilis. Mol Microbiol. 2010;77(1):108-27. PMID: 20487272.

Möbius K, Arias-Cartin R, Breckau D, Hännig AL, Riedmann K, Biedendieck R, Schröder S, Becher D, Magalon A, Moser J, Jahn M, Jahn D. Heme biosynthesis is coupled to electron transport chains for energy generation. Proc Natl Acad Sci U S A. 2010;107(23):10436-41. PMID: 20484676.

Schmidl SR, Gronau K, Pietack N, Hecker M, Becher D, Stülke J. The phosphoproteome of the minimal bacterium Mycoplasma pneumoniae: analysis of the complete known Ser/Thr kinome suggests the existence of novel kinases. Mol Cell Proteomics. 2010;9(6):1228-42. PMID: 20097688.

Sievers S, Ernst CM, Geiger T, Hecker M, Wolz C, Becher D, Peschel A. Changing the phospholipid composition of Staphylococcus aureus causes distinct changes in membrane proteome and membrane-sensory regulators. Proteomics. 2010;10(8):1685-93. PMID: 20162562.

Hempel K, Pané-Farré J, Otto A, Sievers S, Hecker M, Becher D. Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach. J Proteome Res. 2010;9(3):1579-90. PMID: 20108986.

Miller M, Donat S, Rakette S, Stehle T, Kouwen TR, Diks SH, Dreisbach A, Reilman E, Gronau K, Becher D, Peppelenbosch MP, van Dijl JM, Ohlsen K. Staphylococcal PknB as the first prokaryotic representative of the proline-directed kinases. PLoS One. 2010;5(2):e9057. PMID: 20140229.

Hecker M, Becher D, Fuchs S, Engelmann S. A proteomic view of cell physiology and virulence of Staphylococcus aureus. Int J Med Microbiol. 2010;300(2-3):76-87 Review. PMID: 20005169.

Hahne H, Mäder U, Otto A, Bonn F, Steil L, Bremer E, Hecker M, Becher D. A comprehensive proteomics and transcriptomics analysis of Bacillus subtilis salt stress adaptation. J Bacteriol. 2010;192(3):870-82. PMID: 19948795.

Schmidl SR, Gronau K, Hames C, Busse J, Becher D, Hecker M, Stülke J. The stability of cytadherence proteins in Mycoplasma pneumoniae requires activity of the protein kinase PrkC. Infect Immun. 2010 Jan;78(1):184-92. PMID: 19858294.

Pietack N, Becher D, Schmidl SR, Saier MH, Hecker M, Commichau FM, Stülke J. In vitro phosphorylation of key metabolic enzymes from Bacillus subtilis: PrkC phosphorylates enzymes from different branches of basic metabolism. J Mol Microbiol Biotechnol. 2010;18(3):129-40. PMID: 20389117.

Otto A, Bernhardt J, Meyer H, Schaffer M, Herbst FA, Siebourg J, Mäder U, Lalk M, Hecker M, Becher D. Systems-wide temporal proteomic profiling in glucose-starved Bacillus subtilis. Nat Commun. 2010;1:137. PMID: 21266987.


Becher D, Hempel K, Sievers S, Zühlke D, Pané-Farré J, Otto A, Fuchs S, Albrecht D, Bernhardt J, Engelmann S, Völker U, van Dijl JM, Hecker M. A proteomic view of an important human pathogen–towards the quantification of the entire Staphylococcus aureus proteome. PLoS One. 2009;4(12):e8176. PMID: 19997597.

Pöther DC, Liebeke M, Hochgräfe F, Antelmann H, Becher D, Lalk M, Lindequist U, Borovok I, Cohen G, Aharonowitz Y, Hecker M. Diamide triggers mainly S Thiolations in the cytoplasmic proteomes of Bacillus subtilis and Staphylococcus aureus. J Bacteriol. 2009;191(24):7520-30. PMID: 19837798.

Hempel K, Rosen R, Becher D, Büttner K, Hecker M, Ron EZ. Analysis of ultra acidic proteins by the use of anodic acidic gels. Anal Biochem. 2009;385(2):208-14. PMID: 19084495.

Altindiş E, Tefon BE, Yildirim V, Ozcengiz E, Becher D, Hecker M, Ozcengiz G. Immunoproteomic analysis of Bordetella pertussis and identification of new immunogenic proteins. Vaccine. 2009;27(4):542-8. PMID: 19028538.

Hahne H, Wolff S, Hecker M, Becher D. From complementarity to comprehensiveness–targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics. 2008;8(19):4123-36. PMID: 18763711.

Liebeke M, Pöther DC, van Duy N, Albrecht D, Becher D, Hochgräfe F, Lalk M, Hecker M, Antelmann H. Depletion of thiol-containing proteins in response to quinones in Bacillus subtilis. Mol Microbiol. 2008;69(6):1513-29. PMID: 18673455.

Wolff S, Hahne H, Hecker M, Becher D. Complementary analysis of the vegetative membrane proteome of the human pathogen Staphylococcus aureus. Mol Cell Proteomics. 2008;7(8):1460-8. PMID: 18460691.

Dreisbach A, Otto A, Becher D, Hammer E, Teumer A, Gouw JW, Hecker M, Völker U. Monitoring of changes in the membrane proteome during stationary phase adaptation of Bacillus subtilis using in vivo labeling techniques. Proteomics. 2008;8(10):2062-76. PMID: 18491319.

Zweers JC, Barák I, Becher D, Driessen AJ, Hecker M, Kontinen VP, Saller MJ, Vavrová L, van Dijl JM. Towards the development of Bacillus subtilis as a cell factory for membrane proteins and protein complexes. Microb Cell Fact. 2008;7:10. PMID: 18394159.

Hieu CX, Voigt B, Albrecht D, Becher D, Lombardot T, Glöckner FO, Amann R, Hecker M, Schweder T. Detailed proteome analysis of growing cells of the planctomycete Rhodopirellula baltica SH1T. Proteomics. 2008;8(8):1608-23. PMID: 18340632.

Leelakriangsak M, Huyen NT, Töwe S, van Duy N, Becher D, Hecker M, Antelmann H, Zuber P. Regulation of quinone detoxification by the thiol stress sensing DUF24/MarR-like repressor, YodB in Bacillus subtilis. Mol Microbiol. 2008;67(5):1108-24. PMID: 18208493.


Eymann C, Becher D, Bernhardt J, Gronau K, Klutzny A, Hecker M. Dynamics of protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis. Proteomics. 2007;7(19):3509-26. PMID: 17726680.

Hochgräfe F, Mostertz J, Pöther DC, Becher D, Helmann JD, Hecker M. S-cysteinylation is a general mechanism for thiol protection of Bacillus subtilis proteins after oxidative stress. J Biol Chem. 2007;282(36):25981-5. PMID: 17611193.

Wolff S, Antelmann H, Albrecht D, Becher D, Bernhardt J, Bron S, Büttner K, van Dijl JM, Eymann C, Otto A, Tam le T, Hecker M. Towards the entire proteome of the model bacterium Bacillus subtilis by gel-based and gel-free approaches. J Chromatogr B Analyt Technol Biomed Life Sci. 2007;849(1-2):129-40. Review. PMID: 17055787.

Ozcan S, Yildirim V, Kaya L, Albrecht D, Becher D, Hecker M, Ozcengiz G. Phanerochaete chrysosporium soluble proteome as a prelude for the analysis of heavy metal stress response. Proteomics. 2007;7(8):1249-60. PMID: 17366474.

Markert S, Arndt C, Felbeck H, Becher D, Sievert SM, Hügler M, Albrecht D, Robidart J, Bench S, Feldman RA, Hecker M, Schweder T. Physiological proteomics of the uncultured endosymbiont of Riftia pachyptila. Science. 2007;315(5809):247-50. PMID: 17218528.

Borriss M, Lombardot T, Glöckner FO, Becher D, Albrecht D, Schweder T. Genome and proteome characterization of the psychrophilic Flavobacterium bacteriophage 11b. Extremophiles. 2007;11(1):95-104. PMID: 16932843.

2006 and older

Linsen L, Löcherbach J, Berth M, Becher D, Bernhardt J. Visual analysis of gel-free proteome data. IEEE Trans Vis Comput Graph. 2006;12(4):497-508. PMID: 16805259.

Wolff S, Otto A, Albrecht D, Zeng JS, Büttner K, Glückmann M, Hecker M, Becher D. Gel-free and gel-based proteomics in Bacillus subtilis: a comparative study. Mol Cell Proteomics. 2006;5(7):1183-92. PMID: 16552027.

Mattow J, Siejak F, Hagens K, Becher D, Albrecht D, Krah A, Schmidt F, Jungblut PR, Kaufmann SH, Schaible UE. Proteins unique to intraphagosomally grown Mycobacterium tuberculosis. Proteomics. 2006;6(8):2485-94. PMID: 16548060.

Kohler C, Wolff S, Albrecht D, Fuchs S, Becher D, Büttner K, Engelmann S, Hecker M. Proteome analyses of Staphylococcus aureus in growing and non-growing cells: a physiological approach. Int J Med Microbiol. 2005;295(8):547-65. PMID: 16325551.

Wesche J, Hammer E, Becher D, Burchhardt G, Schauer F. The bphC gene-encoded 2,3-dihydroxybiphenyl-1,2-dioxygenase is involved in complete degradation of dibenzofuran by the biphenyl-degrading bacterium Ralstonia sp. SBUG 290. J Appl Microbiol. 2005;98(3):635-45. PMID: 15715866.

Rosen R, Becher D, Büttner K, Biran D, Hecker M, Ron EZ. Probing the active site of homoserine trans-succinylase. FEBS Lett. 2004;577(3):386-92. PMID: 15556615.

Rosen R, Becher D, Büttner K, Biran D, Hecker M, Ron EZ. Highly phosphorylated bacterial proteins. Proteomics. 2004;4(10):3068-77. PMID: 15378765.

Eymann C, Dreisbach A, Albrecht D, Bernhardt J, Becher D, Gentner S, Tam le T, Büttner K, Buurman G, Scharf C, Venz S, Völker U, Hecker M. A comprehensive proteome map of growing Bacillus subtilis cells. Proteomics. 2004;4(10):2849-76. PMID: 15378759.

Ziebandt AK, Becher D, Ohlsen K, Hacker J, Hecker M, Engelmann S. The influence of agr and sigmaB in growth phase dependent regulation of virulence factors in Staphylococcus aureus. Proteomics. 2004 ;4(10):3034-47. PMID: 15378746.

Voigt B, Schweder T, Becher D, Ehrenreich A, Gottschalk G, Feesche J, Maurer KH, Hecker M. A proteomic view of cell physiology of Bacillus licheniformis. Proteomics. 2004;4(5):1465-90. PMID: 15188415.

Weber H, Engelmann S, Becher D, Hecker M. Oxidative stress triggers thiol oxidation in the glyceraldehyde-3-phosphate dehydrogenase of Staphylococcus aureus. Mol Microbiol. 2004;52(1):133-40. PMID: 15049816.

Rosen R, Sacher A, Shechter N, Becher D, Büttner K, Biran D, Hecker M, Ron EZ. Two-dimensional reference map of Agrobacterium tumefaciens proteins. Proteomics. 2004;4(4):1061-73. PMID: 15048987.

Brandt R, Nawka M, Kellermann J, Salazar R, Becher D, Krantz S. Nucleophosmin is a component of the fructoselysine-specific receptor in cell membranes of Mono Mac 6 and U937 monocyte-like cells. Biochim Biophys Acta. 2004;1670(2):132-6. PMID: 14738996.

Krah A, Schmidt F, Becher D, Schmid M, Albrecht D, Rack A, Büttner K, Jungblut PR. Analysis of automatically generated peptide mass fingerprints of cellular proteins and antigens from Helicobacter pylori 26695 separated by two-dimensional electrophoresis. Mol Cell Proteomics. 2003;2(12):1271-83. PMID: 14519719.

Rosen R, Matthysse AG, Becher D, Biran D, Yura T, Hecker M, Ron EZ. Proteome analysis of plant-induced proteins of Agrobacterium tumefaciens. FEMS Microbiol Ecol. 2003;44(3):355-60. PMID: 19719616.

Bandow JE, Becher D, Büttner K, Hochgräfe F, Freiberg C, Brötz H, Hecker M. The role of peptide deformylase in protein biosynthesis: a proteomic study. Proteomics. 2003;3(3):299-306. PMID: 12627383.

Stope MB, Becher D, Hammer E, Schauer F. Cometabolic ring fission of dibenzofuran by Gram-negative and Gram-positive biphenyl-utilizing bacteria. Appl Microbiol Biotechnol. 2002;59(1):62-7. PMID: 12073133.

Rosen R, Büttner K, Becher D, Nakahigashi K, Yura T, Hecker M, Ron EZ. Heat shock proteome of Agrobacterium tumefaciens: evidence for new control systems. J Bacteriol. 2002;184(6):1772-8. PMID: 11872730.

Rosen R, Biran D, Gur E, Becher D, Hecker M, Ron EZ. Protein aggregation in Escherichia coli: role of proteases. FEMS Microbiol Lett. 2002;207(1):9-12. PMID: 11886743.

Becher D, Specht M, Hammer E, Francke W, Schauer F. Cometabolic degradation of dibenzofuran by biphenyl-cultivated Ralstonia sp. strain SBUG 290. Appl Environ Microbiol. 2000;66(10):4528-31. PMID: 11010910.

Scheller U, Zimmer T, Becher D, Schauer F, Schunck WH. Oxygenation cascade in conversion of n-alkanes to alpha,omega-dioic acids catalyzed by cytochrome P450 52A3. J Biol Chem. 1998;273(49):32528-34. PMID: 9829987.

Hundt K, Wagner M, Becher D, Hammer E, Schauer F. Effect of selected environmental factors on degradation and mineralization of biaryl compounds by the bacterium Ralstonia pickettii in soil and compost. Chemosphere. 1998;36(10):2321-35. PMID: 9566302.