Prof. Dr. Michael Hecker (retired)

Microbial Physiology and Molecular Biology

F.- L.- Jahn-Straße 15 - 1. floor - room: 201
17489 Greifswald

phone: +49 (0)3834 420 5905
fax:      +49 (0)3834 420 5909
email:   hecker[at]

opening hours: only by appointment

Curriculum Vitae
1990 – 2013 Full-Professor (C4) and Head of the Institute for Microbiology der Ernst-Moritz-Arndt University Greifswald, Germany
1990 – 1994 Dean of the Faculty for Natural Sciences at the Ernst-Moritz-Arndt-University Greifswald, Germany
1977 Habilitation at the Ernst-Moritz-Arndt-University Greifswald, Germany
1973 Ph.D. (Biochemistry of plants) at the Ernst-Moritz-Arndt-University Greifswald, Germany
1965 – 1970 Studies of Biology, Ernst-Moritz-Arndt-University Greifswald, Germany
1946 Year of birth
Functions in Scientific Societies and Committees, Project Coordination (Selection)
2006 – 2012 Coordinator SFB/TRR34 “Pathophysiology of Staphylococci in the Post-Genomic Era”  
since 2005 Chairman of Competence Center Functional Genomics, BMBF-funded  
since 1995 Member in Editorial Boards of Appl. Microbiol. Biotechnol., Int. J. Med. Microbiol., Environm. Microbiol., J. Bacteriol., Curr. Analyt. Chem., Mol. Gen. Genom., Mol. Biotechnol., Microbial Cell. Fact.; Senior Editor in Proteomics und Proteomics – Clinical Applications  
1995 – 2013 Head of the technology platform “Microbial Proteomics” (EU, 6th and 7th framwork, GenoMik, Pathogenomik, GenoMik-Transfer an other)  
1995 – 2001 President and Vice President of the German Microbiology Society (VAAM)  
1993 – 1996 Reviewer of the German Research Foundation (DFG) for Microbiology  


North German Research Prize


Greifswald University Club Senior Award


Doktor Robert Pfleger Research Award


Chairman of Section 13 and Senat Member of the German Academy of Sciences Leopoldina


Member of the European Academy of Microbiology (EAM)


Corresponding Member of the Academy of Sciences and Humanities Göttingen, Germany

2008 – 2011

Chairman Bacteriology Applied Microbiology Division (BAM) of the International Union of Microbiological Societies (IUMS)


Member of the Academy of Sciences and Humanities Hamburg, Germany


Research / Technology Invention Award, Henkel KGaA


Member of the American Academy of Microbiology (AAM)


Member of the Berlin-Brandenburg Academy of Sciences and Humanities, Germany


Member of the German Academy of Sciences Leopoldina

Major Scientific Interests
  • Physiological proteomics of Bacillus subtilis

Bacillus subtilis is regarded not only as a model organism of Gram positive bacteria but also of functional genomics. Its genome sequence, however, published in 1997 (Kunst et al. 1997) only provides its “blue-print of life”, now functional genomics (transcriptomics, proteomics, metabolomics etc.) is required to bring this genome sequence to cell physiology. The role of proteomics to transfer the “virtual life of the genes to the real life of the proteins” as the main players of life is the main topic of our research program. Proteomes of growing and non-growing cells will provide global information on the regulation of metabolic pathways or on the stress and starvation responses. Stress/starvation proteomic signatures are valuable tools for the prediction of the physiological state of cells or for the prediction of the action mechanisms of unknown drugs.

  • Stress response of Bacillus subtilis: The σB-dependent general stress response

The induction of the σB-dependent stress proteins is one of the most obvious changes in the protein synthesis profile in response to stress and starvation. About 150 genes belong to this σB-dependent general stress regulon. These proteins provide the non-growing B. subtilis cell with a multiple, non-specific and preventive stress resistance in anticipation of “future stress”. The function of the single stress protein in the establishment of a global resistance against heat, ethanol, oxidative, cold, acid, and osmotic stress is currently analysed. Furthermore, the integration of the general stress regulon into a highly sophisticated adaptational network is under study.

  • Proteomics of industrial bacteria (B. licheniformis, B. pumilus and other)
  • A proteomic view of pathogenicity of bacteria (S. aureus and other)
Publication list
  • Junker S, Maaß S, Otto A, Hecker M, Becher D. Toward the quantitative characterization of arginine phosphorylations in Staphylococcus aureus. J Proteome Res. 2018. [Epub ahead of print] PMID: 30358407.
  • Shulman A, Yair Y, Biran D, Sura T, Otto A, Gophna U, Becher D, Hecker M, Ron EZ. The Escherichia coli type III secretion system 2 has a global effect on cell surface. MBio. 2018. 9(4). PMID: 29970469.
  • Eymann C, Wachlin G, Albrecht D, et al. Exoproteome Analysis of Human Pathogenic Dermatophyte Species and Identification of Immunoreactive Proteins. Proteomics Clin Appl. 2018 Jun 28;:e1800007. PMID: 29952123.
  • Handtke S, Albrecht D, Otto A, Becher D, Hecker M and Voigt B. The proteomic response of B. pumilus cells to glucose starvation. Proteomics. 2018. 18(1). PMID: 29193752.
  • Junker S, Maaß S, Otto A, Michalik S, Morgenroth F, Gerth U, Hecker M, Becher D. Spectral library based analysis of arginine phosphorylations in Staphylococcus aureus. Mol Cell Proteomics. 2018. 17(2) 335-348. PMID: 29183913.
  • Hentschker C, Dewald C, Otto A, Büttner K, Hecker M, Becher D. Global quantification of phosphoproteins combining metabolic labeling and gel-based proteomics in B. pumilus. ELECTROPHORESIS. 2017. 39(2):334-343. PMID: 28944503.
  • Junker S, Maaß S, Otto A, Michalik S, Morgenroth F, Gerth U, Hecker M, Becher D. Spectral library based analysis of arginine phosphorylations in Staphylococcus aureusMol Cell Proteomics. 2018 17:335-348.  PMID: 29183913.
  • Handtke S, Albrecht D, Zühlke D, Otto A, Becher D, Schweder T, Riedel K, Hecker M, Voigt B. Bacillus pumilus KatX2 confers enhanced hydrogen peroxide resistance to a Bacillus subtilis PkatA::katX2 mutant strain. Microb Cell Fact. 2017. 16(1):72. PMID: 28446175.

Engelmann S, Hecker M. Pathoproteomics of pathogenic microorganisms. Proteomics Clin Appl.2016 Oct;10(9-10):895-896. PMID: 27723267.

Zühlke D, Dörries K, Bernhardt J, Maaß S, Muntel J, Liebscher V, Pané-Farré J, Riedel K, Lalk M, Völker U, Engelmann S, Becher D, Fuchs S, Hecker M. Costs of life – Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis. Sci Rep.2016 Jun 27;6:28172. PMID: 27344979.

Vu CH, Kolata J,, Stentzel S, Beyer A, Gesell Salazar M, Steil L, Pané-Farré J, Rühmling V, Engelmann S,,, Götz F, van Dijl JM, Hecker M, Mäder U, Schmidt F, Völker U, Bröker BM. Adaptive immune response to lipoproteins of Staphylococcus aureus in healthy subjects. Proteomics.2016 Oct;16(20):2667-2677. PMID: 27324828.

Mäder U, Nicolas P, Depke M, Pané-Farré J, Debarbouille M, van der Kooi-Pol MM, Guérin C, Dérozier S, Hiron A, Jarmer H, Leduc A, Michalik S, Reilman E, Schaffer M, Schmidt F, Bessières P, Noirot P, Hecker M, Msadek T, Völker U, van Dijl JM. Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions. PLoS Genet.2016 Apr 1;12(4):e1005962. PMID: 27035918.

Bonn F, Pané-Farré J, Schlüter R, Schaffer M, Fuchs S, Bernhardt J, Riedel K, Otto A, Völker U, van Dijl JM, Hecker M, Mäder U, Becher D. Global analysis of the impact of linezolid onto virulence factor production in S. aureus USA300. Int J Med Microbiol.2016 Feb 17. PMID: 26996810.

Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Pühler A. Comparative proteome analysis of Actinoplanes sp. SE50/110 grown with maltose or glucose shows minor differences for acarbose biosynthesis proteins but major differences for saccharide transporters. J Proteomics.2016 Jan 10;131:140-8. PMID: 26597626.

Aurass P, Gerlach T, Becher D, Voigt B, Karste S, Bernhardt J, Riedel K, Hecker M, Flieger A. Life Stage-specific Proteomes of Legionella pneumophila Reveal a Highly Differential Abundance of Virulence-associated Dot/Icm effectors. Mol Cell Proteomics.2016 Jan;15(1):177-200. PMID: 26545400.


Goelzer A, Muntel J, Chubukov V, Jules M, Prestel E, Nölker R, Mariadassou M, Aymerich S, Hecker M, Noirot P, Becher D, Fromion V. Quantitative prediction of genome-wide resource allocation in bacteria. Metab Eng.2015 Nov;32:232-43. PMID: 26498510.

Herweg JA, Pons V, Becher D, Hecker M, Krohne G, Barbier J, Berger H, Rudel T, Mehlitz A,. Proteomic analysis of the Simkania-containing vacuole: the central role of retrograde transport. Mol Microbiol.2016 Jan;99(1):151-71. PMID: 26374382.

Depke M, Michalik S, Rabe A, Surmann K, Brinkmann L, Jehmlich N, Bernhardt J, Hecker M, Wollscheid B, Sun Z, Moritz RL, Völker U, Schmidt F. A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies. Proteomics.2015 Nov;15(21):3648-61. PMID: 26224020.

Stentzel S, Sundaramoorthy N, Michalik S, Nordengrün M, Schulz S, Kolata J, Kloppot P, Engelmann S, Steil L, Hecker M, Schmidt F, Völker U, Roghmann MC, Bröker BM. Specific serum IgG at diagnosis of Staphylococcus aureus bloodstream invasion is correlated with disease progression. J Proteomics.2015 Oct 14;128:1-7. PMID: 26155744.

Moche M, Schlüter R, Bernhardt J, Plate K, Riedel K, Hecker M, Becher D. Time-Resolved Analysis of Cytosolic and Surface-Associated Proteins of Staphylococcus aureus HG001 under Planktonic and Biofilm Conditions. J Proteome Res.2015 Sep 4;14(9):3804-22. PMID: 26152824.

Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Walter F, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Pühler A. Comprehensive proteome analysis of Actinoplanes sp. SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster. J Proteomics.2015 Jul 1;125:1-16. PMID: 25896738.

Kohler C,, Lourenço RF, Bernhardt J, Albrecht D, Schüler J, Hecker M, Gomes SL. A comprehensive genomic, transcriptomic and proteomic analysis of a hyperosmotic stress sensitive α-proteobacterium. BMC Microbiol.2015 Mar 26;15:71. PMID: 25879753.

Voigt B, Albrecht D, Sievers S, Becher D, Bongaerts J, Evers S, Schweder T, Maurer KH, Hecker M. High-resolution proteome maps of Bacillus licheniformis cells growing in minimal medium. Proteomics.2015 Aug;15(15):2629-33. PMID: 25867794.

Kloppot P, Selle M, Kohler C, Stentzel S, Fuchs S, Liebscher V, Müller E, Kale D, Ohlsen K, Bröker BM, Zipfel PF,, Kahl BC, Ehricht R, Hecker M, Engelmann S,,. Microarray-based identification of human antibodies against Staphylococcus aureus antigens. Proteomics Clin Appl.2015 Dec;9(11-12):1003-11. PMID: 25676254.

Barbieri G, Voigt B, Albrecht D, Hecker M, Albertini AM, Sonenshein AL, Ferrari E, Belitsky BR. CodY regulates expression of the Bacillus subtilis extracellular proteases Vpr and Mpr. J Bacteriol.2015 Apr;197(8):1423-32. PMID: 25666135.


Nega M, Dube L, Kull M, Ziebandt AK, Ebner P, Albrecht D, Krismer B,Rosenstein R, Hecker M, Götz F. Secretome analysis revealed adaptive and
non-adaptive responses of the Staphylococcus carnosus femB mutant. Proteomics.2014 Nov 28. doi: 10.1002/pmic.201400343. [Epub ahead of print] PubMed PMID:25430637.


Handtke S, Volland S, Methling K, Albrecht D, Becher D, Nehls J, Bongaerts J,

Maurer KH, Lalk M, Liesegang H, Voigt B, Daniel R, Hecker M. Cell physiology of

the biotechnological relevant bacterium Bacillus pumilus-An omics-based approach.

J Biotechnol. 2014 Dec 20;192 Pt A:204-14. doi: 10.1016/j.jbiotec.2014.08.028.

Epub 2014 Oct 2. PubMed PMID: 25281541.


Kohlmann Y, Pohlmann A, Schwartz E, Zühlke D, Otto A, Albrecht D, Grimmler C,

Ehrenreich A, Voigt B, Becher D, Hecker M, Friedrich B, Cramm R. Coping with

anoxia: a comprehensive proteomic and transcriptomic survey of denitrification. J

Proteome Res. 2014 Oct 3;13(10):4325-38. doi: 10.1021/pr500491r. Epub 2014 Sep 8.

PubMed PMID: 25198380.


Huja S, Oren Y, Biran D, Meyer S, Dobrindt U, Bernhard J, Becher D, Hecker M,

Sorek R, Ron EZ. Fur is the master regulator of the extraintestinal pathogenic

Escherichia coli response to serum. MBio. 2014 Aug 12;5(4). pii: e01460-14. doi:

10.1128/mBio.01460-14. PubMed PMID: 25118243; PubMed Central PMCID: PMC4145685.


Müller M, Reiß S, Schlüter R, Mäder U, Beyer A, Reiß W, Marles-Wright J,

Lewis RJ, Pförtner H, Völker U, Riedel K, Hecker M, Engelmann S, Pané-Farré J.

Deletion of membrane-associated Asp23 leads to upregulation of cell wall stress

genes in Staphylococcus aureus. Mol Microbiol. 2014 Sep;93(6):1259-68. doi:

10.1111/mmi.12733. Epub 2014 Aug 19. PubMed PMID: 25074408.


Voigt B, Schroeter R, Schweder T, Jürgen B, Albrecht D, van Dijl JM, Maurer

KH, Hecker M. A proteomic view of cell physiology of the industrial workhorse

Bacillus licheniformis. J Biotechnol. 2014 Dec 10;191:139-49. doi:

10.1016/j.jbiotec.2014.06.004. Epub 2014 Jul 8. PubMed PMID: 25011098.


Bonn F, Bartel J, Büttner K, Hecker M, Otto A, Becher D. Picking vanished

proteins from the void: how to collect and ship/share extremely dilute proteins

in a reproducible and highly efficient manner. Anal Chem. 2014 Aug

5;86(15):7421-7. doi: 10.1021/ac501189j. Epub 2014 Jul 21. PubMed PMID: 24987932.


Grabe HJ, Assel H, Bahls T, Dörr M, Endlich K, Endlich N, Erdmann P, Ewert R,

Felix SB, Fiene B, Fischer T, Flessa S, Friedrich N, Gadebusch-Bondio M, Salazar

MG, Hammer E, Haring R, Havemann C, Hecker M, Hoffmann W, Holtfreter B,

Kacprowski T, Klein K, Kocher T, Kock H, Krafczyk J, Kuhn J, Langanke M,

Lendeckel U, Lerch MM, Lieb W, Lorbeer R, Mayerle J, Meissner K, zu Schwabedissen

HM, Nauck M, Ott K, Rathmann W, Rettig R, Richardt C, Saljé K, Schminke U, Schulz

A, Schwab M, Siegmund W, Stracke S, Suhre K, Ueffing M, Ungerer S, Völker U,

Völzke H, Wallaschofski H, Werner V, Zygmunt MT, Kroemer HK. Cohort profile:

Greifswald approach to individualized medicine (GANI_MED). J Transl Med. 2014 May

23;12:144. doi: 10.1186/1479-5876-12-144. PubMed PMID: 24886498; PubMed Central

PMCID: PMC4040487.


Maaβ S, Wachlin G, Bernhardt J, Eymann C, Fromion V, Riedel K, Becher D,

Hecker M. Highly precise quantification of protein molecules per cell during

stress and starvation responses in Bacillus subtilis. Mol Cell Proteomics. 2014

Sep;13(9):2260-76. doi: 10.1074/mcp.M113.035741. Epub 2014 May 30. PubMed PMID:

24878497; PubMed Central PMCID: PMC4159648.


Brandt U, Waletzko C, Voigt B, Hecker M, Steinbüchel A. Mercaptosuccinate

metabolism in Variovorax paradoxus strain B4–a proteomic approach. Appl

Microbiol Biotechnol. 2014 Jul;98(13):6039-50. doi: 10.1007/s00253-014-5811-7.

Epub 2014 May 17. PubMed PMID: 24839213.


Raberg M, Voigt B, Hecker M, Steinbüchel A. A closer look on the

polyhydroxybutyrate- (PHB-) negative phenotype of Ralstonia eutropha PHB-4. PLoS

One. 2014 May 2;9(5):e95907. doi: 10.1371/journal.pone.0095907. eCollection 2014.

PubMed PMID: 24787649; PubMed Central PMCID: PMC4008487.


Meyer H, Weidmann H, Mäder U, Hecker M, Völker U, Lalk M. A time resolved

metabolomics study: the influence of different carbon sources during growth and

starvation of Bacillus subtilis. Mol Biosyst. 2014 Jul;10(7):1812-23. doi:

10.1039/c4mb00112e. PubMed PMID: 24727859.


Muntel J, Fromion V, Goelzer A, Maaβ S, Mäder U, Büttner K, Hecker M, Becher

  1. Comprehensive absolute quantification of the cytosolic proteome of Bacillus

subtilis by data independent, parallel fragmentation in liquid

chromatography/mass spectrometry (LC/MS(E)). Mol Cell Proteomics. 2014

Apr;13(4):1008-19. doi: 10.1074/mcp.M113.032631. Epub 2014 Jan 31. PubMed PMID:

24696501; PubMed Central PMCID: PMC3977180.


Kohlstedt M, Sappa PK, Meyer H, Maaß S, Zaprasis A, Hoffmann T, Becker J,

Steil L, Hecker M, van Dijl JM, Lalk M, Mäder U, Stülke J, Bremer E, Völker U,

Wittmann C. Adaptation of Bacillus subtilis carbon core metabolism to

simultaneous nutrient limitation and osmotic challenge: a multi-omics

perspective. Environ Microbiol. 2014 Jun;16(6):1898-917. doi:

10.1111/1462-2920.12438. Epub 2014 Mar 31. PubMed PMID: 24571712.


Pförtner H, Burian MS, Michalik S, Depke M, Hildebrandt P, Dhople VM,

Pané-Farré J, Hecker M, Schmidt F, Völker U. Activation of the alternative sigma

factor SigB of Staphylococcus aureus following internalization by epithelial

cells – an in vivo proteomics perspective. Int J Med Microbiol. 2014

Mar;304(2):177-87. doi: 10.1016/j.ijmm.2013.11.014. Epub 2013 Dec 7. PubMed PMID:



Handtke S, Schroeter R, Jürgen B, Methling K, Schlüter R, Albrecht D, van

Hijum SA, Bongaerts J, Maurer KH, Lalk M, Schweder T, Hecker M, Voigt B. Bacillus

pumilus reveals a remarkably high resistance to hydrogen peroxide provoked

oxidative stress. PLoS One. 2014 Jan 20;9(1):e85625. doi:

10.1371/journal.pone.0085625. eCollection 2014. PubMed PMID: 24465625; PubMed

Central PMCID: PMC3896406.


Bäsell K, Otto A, Junker S, Zühlke D, Rappen GM, Schmidt S, Hentschker C,

Macek B, Ohlsen K, Hecker M, Becher D. The phosphoproteome and its physiological

dynamics in Staphylococcus aureus. Int J Med Microbiol. 2014 Mar;304(2):121-32.

doi: 10.1016/j.ijmm.2013.11.020. Epub 2013 Dec 1. PubMed PMID: 24457182.


Bröker B, Hecker M. Pathophysiology of staphylococci in the post-genomic era.

Int J Med Microbiol. 2014 Mar;304(2):101-2. doi: 10.1016/j.ijmm.2013.11.003. Epub

2013 Dec 1. PubMed PMID: 24440359.


Otto A, van Dijl JM, Hecker M, Becher D. The Staphylococcus aureus proteome.

Int J Med Microbiol. 2014 Mar;304(2):110-20. doi: 10.1016/j.ijmm.2013.11.007.

Epub 2013 Dec 1. Review. PubMed PMID: 24439828.


Runde S, Molière N, Heinz A, Maisonneuve E, Janczikowski A, Elsholz AK, Gerth

U, Hecker M, Turgay K. The role of thiol oxidative stress response in

heat-induced protein aggregate formation during thermotolerance in Bacillus

subtilis. Mol Microbiol. 2014 Mar;91(5):1036-52. doi: 10.1111/mmi.12521. Epub

2014 Jan 29. PubMed PMID: 24417481.


Pribyl T, Moche M, Dreisbach A, Bijlsma JJ, Saleh M, Abdullah MR, Hecker M,

van Dijl JM, Becher D, Hammerschmidt S. Influence of impaired lipoprotein

biogenesis on surface and exoproteome of Streptococcus pneumoniae. J Proteome

Res. 2014 Feb 7;13(2):650-67. doi: 10.1021/pr400768v. Epub 2014 Jan 15. PubMed

PMID: 24387739.


Hoffmann C, Finsel I, Otto A, Pfaffinger G, Rothmeier E, Hecker M, Becher D,

Hilbi H. Functional analysis of novel Rab GTPases identified in the proteome of

purified Legionella-containing vacuoles from macrophages. Cell Microbiol. 2014

Jul;16(7):1034-52. doi: 10.1111/cmi.12256. Epub 2014 Jan 27. PubMed PMID:



Schroeter R, Hoffmann T, Voigt B, Meyer H, Bleisteiner M, Muntel J, Jürgen B,

Albrecht D, Becher D, Lalk M, Evers S, Bongaerts J, Maurer KH, Putzer H, Hecker

M, Schweder T, Bremer E. Stress responses of the industrial workhorse Bacillus

licheniformis to osmotic challenges. PLoS One. 2013 Nov 15;8(11):e80956. doi:

10.1371/journal.pone.0080956. eCollection 2013. PubMed PMID: 24348917; PubMed

Central PMCID: PMC3858371.


Wiegand S, Voigt B, Albrecht D, Bongaerts J, Evers S, Hecker M, Daniel R,

Liesegang H. Fermentation stage-dependent adaptations of Bacillus licheniformis

during enzyme production. Microb Cell Fact. 2013 Dec 6;12:120. doi:

10.1186/1475-2859-12-120. PubMed PMID: 24313996; PubMed Central PMCID:



Hessling B, Bonn F, Otto A, Herbst FA, Rappen GM, Bernhardt J, Hecker M,

Becher D. Global proteome analysis of vancomycin stress in Staphylococcus aureus.

Int J Med Microbiol. 2013 Dec;303(8):624-34. doi: 10.1016/j.ijmm.2013.08.014.

Epub 2013 Sep 13. PubMed PMID: 24161710.


Fraunholz M, Bernhardt J, Schuldes J, Daniel R, Hecker M, Sinha B. Complete

Genome Sequence of Staphylococcus aureus 6850, a Highly Cytotoxic and Clinically

Virulent Methicillin-Sensitive Strain with Distant Relatedness to Prototype

Strains. Genome Announc. 2013 Sep 26;1(5). pii: e00775-13. doi:

10.1128/genomeA.00775-13. PubMed PMID: 24072870; PubMed Central PMCID:



Fuchs S, Zühlke D, Pané-Farré J, Kusch H, Wolf C, Reiß S, Binh le TN,

Albrecht D, Riedel K, Hecker M, Engelmann S. Aureolib – a proteome signature

library: towards an understanding of staphylococcus aureus pathophysiology. PLoS

One. 2013 Aug 13;8(8):e70669. doi: 10.1371/journal.pone.0070669. eCollection

  1. PubMed PMID: 23967085; PubMed Central PMCID: PMC3742771.


Winter T, Bernhardt J, Winter J, Mäder U, Schlüter R, Weltmann KD, Hecker M,

Kusch H. Common versus noble Bacillus subtilis differentially responds to air and

argon gas plasma. Proteomics. 2013 Sep;13(17):2608-21. doi:

10.1002/pmic.201200343. Epub 2013 Aug 7. PubMed PMID: 23794223.


Hessling B, Büttner K, Hecker M, Becher D. Global relative quantification

with liquid chromatography-matrix-assisted laser desorption ionization

time-of-flight (LC-MALDI-TOF)–cross-validation with LTQ-Orbitrap proves

reliability and reveals complementary ionization preferences. Mol Cell

Proteomics. 2013 Oct;12(10):2911-20. doi: 10.1074/mcp.M112.023457. Epub 2013 Jun

  1. PubMed PMID: 23788530; PubMed Central PMCID: PMC3790300.


Skovager A, Larsen MH, Castro-Mejia JL, Hecker M, Albrecht D, Gerth U,

Arneborg N, Ingmer H. Initial adhesion of Listeria monocytogenes to fine polished

stainless steel under flow conditions is determined by prior growth conditions.

Int J Food Microbiol. 2013 Jul 1;165(1):35-42. doi:

10.1016/j.ijfoodmicro.2013.04.014. Epub 2013 Apr 26. PubMed PMID: 23685728.


Voigt B, Schroeter R, Jürgen B, Albrecht D, Evers S, Bongaerts J, Maurer KH,

Schweder T, Hecker M. The response of Bacillus licheniformis to heat and ethanol

stress and the role of the SigB regulon. Proteomics. 2013 Jul;13(14):2140-61.

doi: 10.1002/pmic.201200297. PubMed PMID: 23592518.


Pöther DC, Gierok P, Harms M, Mostertz J, Hochgräfe F, Antelmann H, Hamilton

CJ, Borovok I, Lalk M, Aharonowitz Y, Hecker M. Distribution and

infection-related functions of bacillithiol in Staphylococcus aureus. Int J Med

Microbiol. 2013 Apr;303(3):114-23. doi: 10.1016/j.ijmm.2013.01.003. Epub 2013 Mar

  1. PubMed PMID: 23517692.


Moche M, Albrecht D, Maaß S, Hecker M, Westermeier R, Büttner K. The new

horizon in 2D electrophoresis: new technology to increase resolution and

sensitivity. Electrophoresis. 2013 Jun;34(11):1510-8. doi:

10.1002/elps.201200618. PubMed PMID: 23494680.


van Dijl JM, Hecker M. Bacillus subtilis: from soil bacterium to

super-secreting cell factory. Microb Cell Fact. 2013 Jan 14;12:3. doi:

10.1186/1475-2859-12-3. PubMed PMID: 23311580; PubMed Central PMCID: PMC3564730.


Goosens VJ, Otto A, Glasner C, Monteferrante CC, van der Ploeg R, Hecker M,

Becher D, van Dijl JM. Novel twin-arginine translocation pathway-dependent

phenotypes of Bacillus subtilis unveiled by quantitative proteomics. J Proteome

Res. 2013 Feb 1;12(2):796-807. doi: 10.1021/pr300866f. Epub 2013 Jan 11. PubMed

PMID: 23256564.


Feng J, Michalik S, Varming AN, Andersen JH, Albrecht D, Jelsbak L, Krieger

S, Ohlsen K, Hecker M, Gerth U, Ingmer H, Frees D. Trapping and proteomic

identification of cellular substrates of the ClpP protease in Staphylococcus

aureus. J Proteome Res. 2013 Feb 1;12(2):547-58. doi: 10.1021/pr300394r. Epub

2013 Jan 8. PubMed PMID: 23253041.


Golec P, Karczewska-Golec J, Voigt B, Albrecht D, Schweder T, Hecker M,

Wegrzyn G, Łos M. Proteomic profiles and kinetics of development of bacteriophage

T4 and its rI and rIII mutants in slowly growing Escherichia coli. J Gen Virol.

2013 Apr;94(Pt 4):896-905. doi: 10.1099/vir.0.048686-0. Epub 2012 Dec 12. PubMed

PMID: 23239571.


Sibbald MJ, Yang XM, Tsompanidou E, Qu D, Hecker M, Becher D, Buist G, van

Dijl JM. Partially overlapping substrate specificities of staphylococcal group A

sortases. Proteomics. 2012 Oct;12(19-20):3049-62. doi: 10.1002/pmic.201200144.

Epub 2012 Sep 20. PubMed PMID: 22930668.


Reder A, Pöther DC, Gerth U, Hecker M. The modulator of the general stress

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Kaddor C, Voigt B, Hecker M, Steinbüchel A. Impact of the core components of

the phosphoenolpyruvate-carbohydrate phosphotransferase system, HPr and EI, on

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Voigt B, Hieu CX, Hempel K, Becher D, Schlüter R, Teeling H, Glöckner FO,

Amann R, Hecker M, Schweder T. Cell surface proteome of the marine planctomycete

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Reder A, Höper D, Gerth U, Hecker M. Contributions of individual

σB-dependent general stress genes to oxidative stress resistance of Bacillus

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2012 May 11. PubMed PMID: 22582280; PubMed Central PMCID: PMC3393503.


Michalik S, Bernhardt J, Otto A, Moche M, Becher D, Meyer H, Lalk M,

Schurmann C, Schlüter R, Kock H, Gerth U, Hecker M. Life and death of proteins: a

case study of glucose-starved Staphylococcus aureus. Mol Cell Proteomics. 2012

Sep;11(9):558-70. doi: 10.1074/mcp.M112.017004. Epub 2012 May 3. PubMed PMID:

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Teeling H, Fuchs BM, Becher D, Klockow C, Gardebrecht A, Bennke CM, Kassabgy

M, Huang S, Mann AJ, Waldmann J, Weber M, Klindworth A, Otto A, Lange J,

Bernhardt J, Reinsch C, Hecker M, Peplies J, Bockelmann FD, Callies U, Gerdts G,

Wichels A, Wiltshire KH, Glöckner FO, Schweder T, Amann R. Substrate-controlled

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Reder A, Albrecht D, Gerth U, Hecker M. Cross-talk between the general

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Oct;14(10):2741-56. doi: 10.1111/j.1462-2920.2012.02755.x. Epub 2012 Apr 24.

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Kleiner M, Wentrup C, Lott C, Teeling H, Wetzel S, Young J, Chang YJ, Shah

M, VerBerkmoes NC, Zarzycki J, Fuchs G, Markert S, Hempel K, Voigt B, Becher D,

Liebeke M, Lalk M, Albrecht D, Hecker M, Schweder T, Dubilier N. Metaproteomics

of a gutless marine worm and its symbiotic microbial community reveal unusual

pathways for carbon and energy use. Proc Natl Acad Sci U S A. 2012 May

8;109(19):E1173-82. doi: 10.1073/pnas.1121198109. Epub 2012 Apr 18. PubMed PMID:

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Elsholz AK, Turgay K, Michalik S, Hessling B, Gronau K, Oertel D, Mäder U,

Bernhardt J, Becher D, Hecker M, Gerth U. Global impact of protein arginine

phosphorylation on the physiology of Bacillus subtilis. Proc Natl Acad Sci U S A.

2012 May 8;109(19):7451-6. doi: 10.1073/pnas.1117483109. Epub 2012 Apr 19. PubMed

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Poehlein A, Schmidt S, Kaster AK, Goenrich M, Vollmers J, Thürmer A, Bertsch

J, Schuchmann K, Voigt B, Hecker M, Daniel R, Thauer RK, Gottschalk G, Müller V.

An ancient pathway combining carbon dioxide fixation with the generation and

utilization of a sodium ion gradient for ATP synthesis. PLoS One.

2012;7(3):e33439. doi: 10.1371/journal.pone.0033439. Epub 2012 Mar 29. PubMed

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Otto A, Bernhardt J, Hecker M, Becher D. Global relative and absolute

quantitation in microbial proteomics. Curr Opin Microbiol. 2012 Jun;15(3):364-72.

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Nicolas P, Mäder U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, Bidnenko E,

Marchadier E, Hoebeke M, Aymerich S, Becher D, Bisicchia P, Botella E, Delumeau

O, Doherty G, Denham EL, Fogg MJ, Fromion V, Goelzer A, Hansen A, Härtig E,

Harwood CR, Homuth G, Jarmer H, Jules M, Klipp E, Le Chat L, Lecointe F, Lewis P,

Liebermeister W, March A, Mars RA, Nannapaneni P, Noone D, Pohl S, Rinn B,

Rügheimer F, Sappa PK, Samson F, Schaffer M, Schwikowski B, Steil L, Stülke J,

Wiegert T, Devine KM, Wilkinson AJ, van Dijl JM, Hecker M, Völker U, Bessières P,

Noirot P. Condition-dependent transcriptome reveals high-level regulatory

architecture in Bacillus subtilis. Science. 2012 Mar 2;335(6072):1103-6. doi:

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Buescher JM, Liebermeister W, Jules M, Uhr M, Muntel J, Botella E, Hessling

B, Kleijn RJ, Le Chat L, Lecointe F, Mäder U, Nicolas P, Piersma S, Rügheimer F,

Becher D, Bessieres P, Bidnenko E, Denham EL, Dervyn E, Devine KM, Doherty G,

Drulhe S, Felicori L, Fogg MJ, Goelzer A, Hansen A, Harwood CR, Hecker M, Hubner

S, Hultschig C, Jarmer H, Klipp E, Leduc A, Lewis P, Molina F, Noirot P, Peres S,

Pigeonneau N, Pohl S, Rasmussen S, Rinn B, Schaffer M, Schnidder J, Schwikowski

B, Van Dijl JM, Veiga P, Walsh S, Wilkinson AJ, Stelling J, Aymerich S, Sauer U.

Global network reorganization during dynamic adaptations of Bacillus subtilis

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Muntel J, Hecker M, Becher D. An exclusion list based label-free proteome

quantification approach using an LTQ Orbitrap. Rapid Commun Mass Spectrom. 2012

Mar 30;26(6):701-9. doi: 10.1002/rcm.6147. PubMed PMID: 22328225.


Brandt U, Raberg M, Voigt B, Hecker M, Steinbüchel A. Elevated

poly(3-hydroxybutyrate) synthesis in mutants of Ralstonia eutropha H16 defective

in lipopolysaccharide biosynthesis. Appl Microbiol Biotechnol. 2012

Jul;95(2):471-83. doi: 10.1007/s00253-012-3909-3. PubMed PMID: 22314517.


Reder A, Gerth U, Hecker M. Integration of σB activity into the

decision-making process of sporulation initiation in Bacillus subtilis. J

Bacteriol. 2012 Mar;194(5):1065-74. doi: 10.1128/JB.06490-11. Epub 2011 Dec 30.

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Nannapaneni P, Hertwig F, Depke M, Hecker M, Mäder U, Völker U, Steil L, van

Hijum SA. Defining the structure of the general stress regulon of Bacillus

subtilis using targeted microarray analysis and random forest classification.

Microbiology. 2012 Mar;158(Pt 3):696-707. doi: 10.1099/mic.0.055434-0. Epub 2011

Dec 15. PubMed PMID: 22174379.


Thürmer A, Voigt B, Angelov A, Albrecht D, Hecker M, Liebl W. Proteomic

analysis of the extremely thermoacidophilic archaeon Picrophilus torridus at pH

and temperature values close to its growth limit. Proteomics. 2011

Dec;11(23):4559-68. doi: 10.1002/pmic.201000829. PubMed PMID: 22114103.


Reiss S, Pané-Farré J, Fuchs S, François P, Liebeke M, Schrenzel J,

Lindequist U, Lalk M, Wolz C, Hecker M, Engelmann S. Global analysis of the

Staphylococcus aureus response to mupirocin. Antimicrob Agents Chemother. 2012

Feb;56(2):787-804. doi: 10.1128/AAC.05363-11. Epub 2011 Nov 21. PubMed PMID:

22106209; PubMed Central PMCID: PMC3264241.


Gardebrecht A, Markert S, Sievert SM, Felbeck H, Thürmer A, Albrecht D,

Wollherr A, Kabisch J, Le Bris N, Lehmann R, Daniel R, Liesegang H, Hecker M,

Schweder T. Physiological homogeneity among the endosymbionts of Riftia

pachyptila and Tevnia jerichonana revealed by proteogenomics. ISME J. 2012

Apr;6(4):766-76. doi: 10.1038/ismej.2011.137. Epub 2011 Oct 20. PubMed PMID:

22011719; PubMed Central PMCID: PMC3309349.


Kolata J, Bode LG, Holtfreter S, Steil L, Kusch H, Holtfreter B, Albrecht D,

Hecker M, Engelmann S, van Belkum A, Völker U, Bröker BM. Distinctive patterns in

the human antibody response to Staphylococcus aureus bacteremia in carriers and

non-carriers. Proteomics. 2011 Oct;11(19):3914-27. doi: 10.1002/pmic.201000760.

Epub 2011 Sep 7. PubMed PMID: 21805632.


Fasehee H, Westers H, Bolhuis A, Antelmann H, Hecker M, Quax WJ, Mirlohi AF,

van Dijl JM, Ahmadian G. Functional analysis of the sortase YhcS in Bacillus

subtilis. Proteomics. 2011 Oct;11(19):3905-13. doi: 10.1002/pmic.201100174. Epub

2011 Aug 31. PubMed PMID: 21800427.


Hecker M. Microbial proteomics. Proteomics. 2011 Aug;11(15):2941-2. doi:

10.1002/pmic.201190072. PubMed PMID: 21766457.


Winter T, Winter J, Polak M, Kusch K, Mäder U, Sietmann R, Ehlbeck J, van

Hijum S, Weltmann KD, Hecker M, Kusch H. Characterization of the global impact of

low temperature gas plasma on vegetative microorganisms. Proteomics. 2011

Sep;11(17):3518-30. doi: 10.1002/pmic.201000637. Epub 2011 Aug 4. PubMed PMID:



Miller M, Dreisbach A, Otto A, Becher D, Bernhardt J, Hecker M,

Peppelenbosch MP, van Dijl JM. Mapping of interactions between human macrophages

and Staphylococcus aureus reveals an involvement of MAP kinase signaling in the

host defense. J Proteome Res. 2011 Sep 2;10(9):4018-32. doi: 10.1021/pr200224x.

Epub 2011 Jul 28. PubMed PMID: 21736355.


Markert S, Gardebrecht A, Felbeck H, Sievert SM, Klose J, Becher D, Albrecht

D, Thürmer A, Daniel R, Kleiner M, Hecker M, Schweder T. Status quo in

physiological proteomics of the uncultured Riftia pachyptila endosymbiont.

Proteomics. 2011 Aug;11(15):3106-17. doi: 10.1002/pmic.201100059. Epub 2011 Jun

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Delumeau O, Lecointe F, Muntel J, Guillot A, Guédon E, Monnet V, Hecker M,

Becher D, Polard P, Noirot P. The dynamic protein partnership of RNA polymerase

in Bacillus subtilis. Proteomics. 2011 Aug;11(15):2992-3001. doi:

10.1002/pmic.201000790. Epub 2011 Jun 28. PubMed PMID: 21710567.


Becher D, Büttner K, Moche M, Hessling B, Hecker M. From the genome sequence

to the protein inventory of Bacillus subtilis. Proteomics. 2011

Aug;11(15):2971-80. doi: 10.1002/pmic.201100090. Epub 2011 Jun 28. Review. PubMed

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Dreisbach A, van der Kooi-Pol MM, Otto A, Gronau K, Bonarius HP, Westra H,

Groen H, Becher D, Hecker M, van Dijl JM. Surface shaving as a versatile tool to

profile global interactions between human serum proteins and the Staphylococcus

aureus cell surface. Proteomics. 2011 Jul;11(14):2921-30. doi:

10.1002/pmic.201100134. Epub 2011 Jun 14. PubMed PMID: 21674804.


Schroeter R, Voigt B, Jürgen B, Methling K, Pöther DC, Schäfer H, Albrecht

D, Mostertz J, Mäder U, Evers S, Maurer KH, Lalk M, Mascher T, Hecker M, Schweder

  1. The peroxide stress response of Bacillus licheniformis. Proteomics. 2011

Jul;11(14):2851-66. doi: 10.1002/pmic.201000461. Epub 2011 Jun 14. PubMed PMID:



Elsholz AK, Hempel K, Michalik S, Gronau K, Becher D, Hecker M, Gerth U.

Activity control of the ClpC adaptor McsB in Bacillus subtilis. J Bacteriol. 2011

Aug;193(15):3887-93. doi: 10.1128/JB.00079-11. Epub 2011 May 27. PubMed PMID:

21622759; PubMed Central PMCID: PMC3147538.


Kohlmann Y, Pohlmann A, Otto A, Becher D, Cramm R, Lütte S, Schwartz E,

Hecker M, Friedrich B. Analyses of soluble and membrane proteomes of Ralstonia

eutropha H16 reveal major changes in the protein complement in adaptation to

lithoautotrophy. J Proteome Res. 2011 Jun 3;10(6):2767-76. doi:

10.1021/pr101289v. Epub 2011 May 11. PubMed PMID: 21561103.


Borgmeier C, Voigt B, Hecker M, Meinhardt F. Functional analysis of the

response regulator DegU in Bacillus megaterium DSM319 and comparative secretome

analysis of degSU mutants. Appl Microbiol Biotechnol. 2011 Aug;91(3):699-711.

doi: 10.1007/s00253-011-3302-7. Epub 2011 May 3. PubMed PMID: 21538108.


van der Ploeg R, Mäder U, Homuth G, Schaffer M, Denham EL, Monteferrante CG,

Miethke M, Marahiel MA, Harwood CR, Winter T, Hecker M, Antelmann H, van Dijl JM.

Environmental salinity determines the specificity and need for Tat-dependent

secretion of the YwbN protein in Bacillus subtilis. PLoS One. 2011 Mar

30;6(3):e18140. doi: 10.1371/journal.pone.0018140. PubMed PMID: 21479178; PubMed

Central PMCID: PMC3068169.


Liang C, Liebeke M, Schwarz R, Zühlke D, Fuchs S, Menschner L, Engelmann S,

Wolz C, Jaglitz S, Bernhardt J, Hecker M, Lalk M, Dandekar T. Staphylococcus

aureus physiological growth limitations: insights from flux calculations built on

proteomics and external metabolite data. Proteomics. 2011 May;11(10):1915-35.

doi: 10.1002/pmic.201000151. Epub 2011 Apr 7. PubMed PMID: 21472852.


Kusch K, Hanke K, Holtfreter S, Schmudde M, Kohler C, Erck C, Wehland J,

Hecker M, Ohlsen K, Bröker B, Engelmann S. The influence of SaeRS and σ(B) on the

expression of superantigens in different Staphylococcus aureus isolates. Int J

Med Microbiol. 2011 Aug;301(6):488-99. doi: 10.1016/j.ijmm.2011.01.003. Epub 2011

Apr 6. PubMed PMID: 21470910.


Tefon BE, Maass S, Ozcengiz E, Becher D, Hecker M, Ozcengiz G. A

comprehensive analysis of Bordetella pertussis surface proteome and

identification of new immunogenic proteins. Vaccine. 2011 Apr 27;29(19):3583-95.

doi: 10.1016/j.vaccine.2011.02.086. Epub 2011 Mar 29. PubMed PMID: 21397717.


Maass S, Sievers S, Zühlke D, Kuzinski J, Sappa PK, Muntel J, Hessling B,

Bernhardt J, Sietmann R, Völker U, Hecker M, Becher D. Efficient, global-scale

quantification of absolute protein amounts by integration of targeted mass

spectrometry and two-dimensional gel-based proteomics. Anal Chem. 2011 Apr

1;83(7):2677-84. doi: 10.1021/ac1031836. Epub 2011 Mar 11. PubMed PMID: 21395229.


Yıldırım V, Ozcan S, Becher D, Büttner K, Hecker M, Ozcengiz G.

Characterization of proteome alterations in Phanerochaete chrysosporium in

response to lead exposure. Proteome Sci. 2011 Mar 9;9:12. doi:

10.1186/1477-5956-9-12. PubMed PMID: 21388532; PubMed Central PMCID: PMC3063812.


Eymann C, Schulz S, Gronau K, Becher D, Hecker M, Price CW. In vivo

phosphorylation patterns of key stressosome proteins define a second feedback

loop that limits activation of Bacillus subtilis σB. Mol Microbiol. 2011

May;80(3):798-810. doi: 10.1111/j.1365-2958.2011.07609.x. Epub 2011 Mar 16.

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Liebeke M, Dörries K, Zühlke D, Bernhardt J, Fuchs S, Pané-Farré J,

Engelmann S, Völker U, Bode R, Dandekar T, Lindequist U, Hecker M, Lalk M. A

metabolomics and proteomics study of the adaptation of Staphylococcus aureus to

glucose starvation. Mol Biosyst. 2011 Apr;7(4):1241-53. doi: 10.1039/c0mb00315h.

Epub 2011 Feb 16. PubMed PMID: 21327190.


Hempel K, Herbst FA, Moche M, Hecker M, Becher D. Quantitative proteomic

view on secreted, cell surface-associated, and cytoplasmic proteins of the

methicillin-resistant human pathogen Staphylococcus aureus under iron-limited

conditions. J Proteome Res. 2011 Apr 1;10(4):1657-66. doi: 10.1021/pr1009838.

Epub 2011 Mar 16. PubMed PMID: 21323324.


Raberg M, Bechmann J, Brandt U, Schlüter J, Uischner B, Voigt B, Hecker M,

Steinbüchel A. Versatile metabolic adaptations of Ralstonia eutropha H16 to a

loss of PdhL, the E3 component of the pyruvate dehydrogenase complex. Appl

Environ Microbiol. 2011 Apr;77(7):2254-63. doi: 10.1128/AEM.02360-10. Epub 2011

Feb 4. PubMed PMID: 21296938; PubMed Central PMCID: PMC3067459.


Raberg M, Peplinski K, Heiss S, Ehrenreich A, Voigt B, Döring C, Bömeke M,

Hecker M, Steinbüchel A. Proteomic and transcriptomic elucidation of the mutant

ralstonia eutropha G+1 with regard to glucose utilization. Appl Environ

Microbiol. 2011 Mar;77(6):2058-70. doi: 10.1128/AEM.02015-10. Epub 2011 Jan 28.

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Otto A, Bernhardt J, Meyer H, Schaffer M, Herbst FA, Siebourg J, Mäder U,

Lalk M, Hecker M, Becher D. Systems-wide temporal proteomic profiling in

glucose-starved Bacillus subtilis. Nat Commun. 2010;1:137. doi:

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Elsholz AK, Hempel K, Pöther DC, Becher D, Hecker M, Gerth U. CtsR

inactivation during thiol-specific stress in low GC, Gram+ bacteria. Mol

Microbiol. 2011 Feb;79(3):772-85. doi: 10.1111/j.1365-2958.2010.07489.x. Epub

2011 Jan 5. PubMed PMID: 21208299.


Saller MJ, Otto A, Berrelkamp-Lahpor GA, Becher D, Hecker M, Driessen AJ.

Bacillus subtilis YqjG is required for genetic competence development.

Proteomics. 2011 Jan;11(2):270-82. doi: 10.1002/pmic.201000435. Epub 2010 Dec 10.

PubMed PMID: 21204254.


Wilmes B, Kock H, Glagla S, Albrecht D, Voigt B, Markert S, Gardebrecht A,

Bode R, Danchin A, Feller G, Hecker M, Schweder T. Cytoplasmic and periplasmic

proteomic signatures of exponentially growing cells of the psychrophilic

bacterium Pseudoalteromonas haloplanktis TAC125. Appl Environ Microbiol. 2011

Feb;77(4):1276-83. doi: 10.1128/AEM.01750-10. Epub 2010 Dec 23. PubMed PMID:

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Elsholz AK, Gerth U, Hecker M. Regulation of CtsR activity in low GC, Gram+

bacteria. Adv Microb Physiol. 2010;57:119-44. doi:

10.1016/B978-0-12-381045-8.00003-5. Review. PubMed PMID: 21078442.

Zdziarski J, Brzuszkiewicz E, Wullt B, Liesegang H, Biran D, Voigt B,

Grönberg-Hernandez J, Ragnarsdottir B, Hecker M, Ron EZ, Daniel R, Gottschalk G,

Hacker J, Svanborg C, Dobrindt U. Host imprints on bacterial genomes–rapid,

divergent evolution in individual patients. PLoS Pathog. 2010 Aug

26;6(8):e1001078. doi: 10.1371/journal.ppat.1001078. PubMed PMID: 20865122;

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Elsholz AK, Michalik S, Zühlke D, Hecker M, Gerth U. CtsR, the Gram-positive

master regulator of protein quality control, feels the heat. EMBO J. 2010 Nov

3;29(21):3621-9. doi: 10.1038/emboj.2010.228. Epub 2010 Sep 17. PubMed PMID:

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Fanous A, Hecker M, Görg A, Parlar H, Jacob F. Corynebacterium glutamicum as

an indicator for environmental cobalt and silver stress–a proteome analysis. J

Environ Sci Health B. 2010 Oct;45(7):666-75. doi: 10.1080/03601234.2010.502442.

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Price CE, Otto A, Fusetti F, Becher D, Hecker M, Driessen AJ. Differential

effect of YidC depletion on the membrane proteome of Escherichia coli under

aerobic and anaerobic growth conditions. Proteomics. 2010 Sep;10(18):3235-47.

doi: 10.1002/pmic.201000284. PubMed PMID: 20706981.


Dreisbach A, Hempel K, Buist G, Hecker M, Becher D, van Dijl JM. Profiling

the surfacome of Staphylococcus aureus. Proteomics. 2010 Sep;10(17):3082-96. doi:

10.1002/pmic.201000062. PubMed PMID: 20662103.


Fuchs S, Mehlan H, Kusch H, Teumer A, Zühlke D, Berth M, Wolf C, Dandekar T,

Hecker M, Engelmann S, Bernhardt J. Protecs, a comprehensive and powerful storage

and analysis system for OMICS data, applied for profiling the anaerobiosis

response of Staphylococcus aureus COL. Proteomics. 2010 Aug;10(16):2982-3000.

doi: 10.1002/pmic.200900388. PubMed PMID: 20662099.


Chi BK, Albrecht D, Gronau K, Becher D, Hecker M, Antelmann H. The

redox-sensing regulator YodB senses quinones and diamide via a thiol-disulfide

switch in Bacillus subtilis. Proteomics. 2010 Sep;10(17):3155-64. doi:

10.1002/pmic.201000230. PubMed PMID: 20652907.


Wolf D, Kalamorz F, Wecke T, Juszczak A, Mäder U, Homuth G, Jordan S,

Kirstein J, Hoppert M, Voigt B, Hecker M, Mascher T. In-depth profiling of the

LiaR response of Bacillus subtilis. J Bacteriol. 2010 Sep;192(18):4680-93. doi:

10.1128/JB.00543-10. Epub 2010 Jul 16. PubMed PMID: 20639339; PubMed Central

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Chi BK, Kobayashi K, Albrecht D, Hecker M, Antelmann H. The paralogous

MarR/DUF24-family repressors YodB and CatR control expression of the catechol

dioxygenase CatE in Bacillus subtilis. J Bacteriol. 2010 Sep;192(18):4571-81.

doi: 10.1128/JB.00409-10. Epub 2010 Jul 16. PubMed PMID: 20639328; PubMed Central

PMCID: PMC2937424.


Janssen H, Döring C, Ehrenreich A, Voigt B, Hecker M, Bahl H, Fischer RJ. A

proteomic and transcriptional view of acidogenic and solventogenic steady-state

cells of Clostridium acetobutylicum in a chemostat culture. Appl Microbiol

Biotechnol. 2010 Aug;87(6):2209-26. doi: 10.1007/s00253-010-2741-x. Epub 2010 Jul

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Thanh TN, Jürgen B, Bauch M, Liebeke M, Lalk M, Ehrenreich A, Evers S,

Maurer KH, Antelmann H, Ernst F, Homuth G, Hecker M, Schweder T. Regulation of

acetoin and 2,3-butanediol utilization in Bacillus licheniformis. Appl Microbiol

Biotechnol. 2010 Aug;87(6):2227-35. doi: 10.1007/s00253-010-2681-5. Epub 2010 Jun

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Jürgen B, Breitenstein A, Urlacher V, Büttner K, Lin H, Hecker M, Schweder

T, Neubauer P. Quality control of inclusion bodies in Escherichia coli. Microb

Cell Fact. 2010 May 28;9:41. doi: 10.1186/1475-2859-9-41. PubMed PMID: 20509924;

PubMed Central PMCID: PMC2893105.


Joshi MV, Mann SG, Antelmann H, Widdick DA, Fyans JK, Chandra G, Hutchings

MI, Toth I, Hecker M, Loria R, Palmer T. The twin arginine protein transport

pathway exports multiple virulence proteins in the plant pathogen Streptomyces

scabies. Mol Microbiol. 2010 Jul 1;77(1):252-71. doi:

10.1111/j.1365-2958.2010.07206.x. Epub 2010 May 12. PubMed PMID: 20487278.


Sibbald MJ, Winter T, van der Kooi-Pol MM, Buist G, Tsompanidou E, Bosma T,

Schäfer T, Ohlsen K, Hecker M, Antelmann H, Engelmann S, van Dijl JM. Synthetic

effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus

aureus. J Bacteriol. 2010 Jul;192(14):3788-800. doi: 10.1128/JB.01452-09. Epub

2010 May 14. PubMed PMID: 20472795; PubMed Central PMCID: PMC2897339.


Pietack N, Becher D, Schmidl SR, Saier MH, Hecker M, Commichau FM, Stülke J.

In vitro phosphorylation of key metabolic enzymes from Bacillus subtilis: PrkC

phosphorylates enzymes from different branches of basic metabolism. J Mol

Microbiol Biotechnol. 2010;18(3):129-40. doi: 10.1159/000308512. Epub 2010 Apr

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Pagels M, Fuchs S, Pané-Farré J, Kohler C, Menschner L, Hecker M, McNamarra

PJ, Bauer MC, von Wachenfeldt C, Liebeke M, Lalk M, Sander G, von Eiff C, Proctor

RA, Engelmann S. Redox sensing by a Rex-family repressor is involved in the

regulation of anaerobic gene expression in Staphylococcus aureus. Mol Microbiol.

2010 Jun 1;76(5):1142-61. doi: 10.1111/j.1365-2958.2010.07105.x. Epub 2010 Mar

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Herbert S, Ziebandt AK, Ohlsen K, Schäfer T, Hecker M, Albrecht D, Novick R,

Götz F. Repair of global regulators in Staphylococcus aureus 8325 and comparative

analysis with other clinical isolates. Infect Immun. 2010 Jun;78(6):2877-89. doi:

10.1128/IAI.00088-10. Epub 2010 Mar 8. PubMed PMID: 20212089; PubMed Central

PMCID: PMC2876537.


Ziebandt AK, Kusch H, Degner M, Jaglitz S, Sibbald MJ, Arends JP, Chlebowicz

MA, Albrecht D, Pantucek R, Doskar J, Ziebuhr W, Bröker BM, Hecker M, van Dijl

JM, Engelmann S. Proteomics uncovers extreme heterogeneity in the Staphylococcus

aureus exoproteome due to genomic plasticity and variant gene regulation.

Proteomics. 2010 Apr;10(8):1634-44. doi: 10.1002/pmic.200900313. PubMed PMID:



Sievers S, Ernst CM, Geiger T, Hecker M, Wolz C, Becher D, Peschel A.

Changing the phospholipid composition of Staphylococcus aureus causes distinct

changes in membrane proteome and membrane-sensory regulators. Proteomics. 2010

Apr;10(8):1685-93. doi: 10.1002/pmic.200900772. PubMed PMID: 20162562.


Hempel K, Pané-Farré J, Otto A, Sievers S, Hecker M, Becher D. Quantitative

cell surface proteome profiling for SigB-dependent protein expression in the

human pathogen Staphylococcus aureus via biotinylation approach. J Proteome Res.

2010 Mar 5;9(3):1579-90. doi: 10.1021/pr901143a. PubMed PMID: 20108986.


Schmidl SR, Gronau K, Pietack N, Hecker M, Becher D, Stülke J. The

phosphoproteome of the minimal bacterium Mycoplasma pneumoniae: analysis of the

complete known Ser/Thr kinome suggests the existence of novel kinases. Mol Cell

Proteomics. 2010 Jun;9(6):1228-42. doi: 10.1074/mcp.M900267-MCP200. Epub 2010 Jan

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Hecker M, Becher D, Fuchs S, Engelmann S. A proteomic view of cell

physiology and virulence of Staphylococcus aureus. Int J Med Microbiol. 2010

Feb;300(2-3):76-87. doi: 10.1016/j.ijmm.2009.10.006. Epub 2009 Dec 11. Review.

PubMed PMID: 20005169.


Becher D, Hempel K, Sievers S, Zühlke D, Pané-Farré J, Otto A, Fuchs S,

Albrecht D, Bernhardt J, Engelmann S, Völker U, van Dijl JM, Hecker M. A

proteomic view of an important human pathogen–towards the quantification of the

entire Staphylococcus aureus proteome. PLoS One. 2009 Dec 4;4(12):e8176. doi:

10.1371/journal.pone.0008176. PubMed PMID: 19997597; PubMed Central PMCID:



Quiel A, Jürgen B, Piechotta G, Le Foll AP, Ziebandt AK, Kohler C, Köster D,

Engelmann S, Erck C, Hintsche R, Wehland J, Hecker M, Schweder T. Electrical

protein array chips for the detection of staphylococcal virulence factors. Appl

Microbiol Biotechnol. 2010 Feb;85(5):1619-27. doi: 10.1007/s00253-009-2347-3.

Epub 2009 Dec 2. PubMed PMID: 19957082.


Hahne H, Mäder U, Otto A, Bonn F, Steil L, Bremer E, Hecker M, Becher D. A

comprehensive proteomics and transcriptomics analysis of Bacillus subtilis salt

stress adaptation. J Bacteriol. 2010 Feb;192(3):870-82. doi: 10.1128/JB.01106-09.

Epub 2009 Nov 30. PubMed PMID: 19948795; PubMed Central PMCID: PMC2812467.


Liedert C, Bernhardt J, Albrecht D, Voigt B, Hecker M, Salkinoja-Salonen M,

Neubauer P. Two-dimensional proteome reference map for the radiation-resistant

bacterium Deinococcus geothermalis. Proteomics. 2010 Feb;10(3):555-63. doi:

10.1002/pmic.200800657. PubMed PMID: 19941306.


Schmidl SR, Gronau K, Hames C, Busse J, Becher D, Hecker M, Stülke J. The

stability of cytadherence proteins in Mycoplasma pneumoniae requires activity of

the protein kinase PrkC. Infect Immun. 2010 Jan;78(1):184-92. doi:

10.1128/IAI.00958-09. Epub 2009 Oct 26. PubMed PMID: 19858294; PubMed Central

PMCID: PMC2798226.


Pöther DC, Liebeke M, Hochgräfe F, Antelmann H, Becher D, Lalk M, Lindequist

U, Borovok I, Cohen G, Aharonowitz Y, Hecker M. Diamide triggers mainly S

Thiolations in the cytoplasmic proteomes of Bacillus subtilis and Staphylococcus

aureus. J Bacteriol. 2009 Dec;191(24):7520-30. doi: 10.1128/JB.00937-09. Epub

2009 Oct 16. PubMed PMID: 19837798; PubMed Central PMCID: PMC2786588.


Götz F, Hacker J, Hecker M. Pathophysiology of staphylococci in the

post-genomic era. Int J Med Microbiol. 2010 Feb;300(2-3):75. doi:

10.1016/j.ijmm.2009.08.012. PubMed PMID: 19836996.


Schwartz E, Voigt B, Zühlke D, Pohlmann A, Lenz O, Albrecht D, Schwarze A,

Kohlmann Y, Krause C, Hecker M, Friedrich B. A proteomic view of the

facultatively chemolithoautotrophic lifestyle of Ralstonia eutropha H16.

Proteomics. 2009 Nov;9(22):5132-42. doi: 10.1002/pmic.200900333. PubMed PMID:



Holtfreter S, Nguyen TT, Wertheim H, Steil L, Kusch H, Truong QP, Engelmann

S, Hecker M, Völker U, van Belkum A, Bröker BM. Human immune proteome in

experimental colonization with Staphylococcus aureus. Clin Vaccine Immunol. 2009

Nov;16(11):1607-14. doi: 10.1128/CVI.00263-09. Epub 2009 Sep 16. PubMed PMID:

19759252; PubMed Central PMCID: PMC2772373.


Michalik S, Liebeke M, Zühlke D, Lalk M, Bernhardt J, Gerth U, Hecker M.

Proteolysis during long-term glucose starvation in Staphylococcus aureus COL.

Proteomics. 2009 Oct;9(19):4468-77. doi: 10.1002/pmic.200900168. PubMed PMID:



Engelmann S, Hecker M. A Proteomics View of Virulence Factors of

Staphylococcus aureus. Genome Dyn. 2009;6:187-97. doi: 10.1159/000235771. Epub

2009 Aug 19. PubMed PMID: 19696502.


Shevchuk O, Batzilla C, Hägele S, Kusch H, Engelmann S, Hecker M, Haas A,

Heuner K, Glöckner G, Steinert M. Proteomic analysis of Legionella-containing

phagosomes isolated from Dictyostelium. Int J Med Microbiol. 2009

Nov;299(7):489-508. doi: 10.1016/j.ijmm.2009.03.006. Epub 2009 May 23. PubMed

PMID: 19482547.


Hecker M, Reder A, Fuchs S, Pagels M, Engelmann S. Physiological proteomics

and stress/starvation responses in Bacillus subtilis and Staphylococcus aureus.

Res Microbiol. 2009 May;160(4):245-58. doi: 10.1016/j.resmic.2009.03.008. Epub

2009 May 3. Review. PubMed PMID: 19403106.


Eberhardt C, Engelmann S, Kusch H, Albrecht D, Hecker M, Autenrieth IB,

Kempf VA. Proteomic analysis of the bacterial pathogen Bartonella henselae and

identification of immunogenic proteins for serodiagnosis. Proteomics. 2009

Apr;9(7):1967-81. doi: 10.1002/pmic.200700670. PubMed PMID: 19333998.


Liebeke M, Brözel VS, Hecker M, Lalk M. Chemical characterization of soil

extract as growth media for the ecophysiological study of bacteria. Appl

Microbiol Biotechnol. 2009 May;83(1):161-73. doi: 10.1007/s00253-009-1965-0. Epub

2009 Mar 24. PubMed PMID: 19308401.


Chatterjee I, Schmitt S, Batzilla CF, Engelmann S, Keller A, Ring MW,

Kautenburger R, Ziebuhr W, Hecker M, Preissner KT, Bischoff M, Proctor RA, Beck

HP, Lenhof HP, Somerville GA, Herrmann M. Staphylococcus aureus ClpC ATPase is a

late growth phase effector of metabolism and persistence. Proteomics. 2009

Mar;9(5):1152-76. doi: 10.1002/pmic.200800586. PubMed PMID: 19253280.


Pané-Farré J, Jonas B, Hardwick SW, Gronau K, Lewis RJ, Hecker M, Engelmann S,

Role of RsbU in controlling SigB activity in Staphylococcus aureus following

alkaline stress. J Bacteriol. 2009 Apr;191(8):2561-73. doi: 10.1128/JB.01514-08.

Epub 2009 Feb 6. PubMed PMID: 19201800; PubMed Central PMCID: PMC2668408.


Kouwen TR, van der Ploeg R, Antelmann H, Hecker M, Homuth G, Mäder U, van

Dijl JM. Overflow of a hyper-produced secretory protein from the Bacillus Sec

pathway into the Tat pathway for protein secretion as revealed by proteogenomics.

Proteomics. 2009 Feb;9(4):1018-32. doi: 10.1002/pmic.200800580. PubMed PMID:



Nguyen TT, Eiamphungporn W, Mäder U, Liebeke M, Lalk M, Hecker M, Helmann

JD, Antelmann H. Genome-wide responses to carbonyl electrophiles in Bacillus

subtilis: control of the thiol-dependent formaldehyde dehydrogenase AdhA and

cysteine proteinase YraA by the MerR-family regulator YraB (AdhR). Mol Microbiol.

2009 Feb;71(4):876-94. doi: 10.1111/j.1365-2958.2008.06568.x. Epub 2008 Dec 23.

PubMed PMID: 19170879.


Wecke T, Zühlke D, Mäder U, Jordan S, Voigt B, Pelzer S, Labischinski H,

Homuth G, Hecker M, Mascher T. Daptomycin versus Friulimicin B: in-depth

profiling of Bacillus subtilis cell envelope stress responses. Antimicrob Agents

Chemother. 2009 Apr;53(4):1619-23. doi: 10.1128/AAC.01046-08. Epub 2009 Jan 21.

PubMed PMID: 19164157; PubMed Central PMCID: PMC2663105.


Kouwen TR, Antelmann H, van der Ploeg R, Denham EL, Hecker M, van Dijl JM.

MscL of Bacillus subtilis prevents selective release of cytoplasmic proteins in a

hypotonic environment. Proteomics. 2009 Feb;9(4):1033-43. doi:

10.1002/pmic.200800483. PubMed PMID: 19160392.


Hempel K, Rosen R, Becher D, Büttner K, Hecker M, Ron EZ. Analysis of ultra

acidic proteins by the use of anodic acidic gels. Anal Biochem. 2009 Feb

15;385(2):208-14. doi: 10.1016/j.ab.2008.11.013. Epub 2008 Nov 19. PubMed PMID:



Altindiş E, Tefon BE, Yildirim V, Ozcengiz E, Becher D, Hecker M, Ozcengiz

  1. Immunoproteomic analysis of Bordetella pertussis and identification of new

immunogenic proteins. Vaccine. 2009 Jan 22;27(4):542-8. doi:

10.1016/j.vaccine.2008.11.020. Epub 2008 Nov 24. PubMed PMID: 19028538.


Hempel K, Rosen R, Becher D, Büttner K, Hecker M, Ron EZ. Analysis of ultra

acidic proteins by the use of anodic acidic gels. Anal Biochem. 2009 Feb

15;385(2):208-14. doi: 10.1016/j.ab.2008.11.013. Epub 2008 Nov 19. PubMed PMID:



Altindiş E, Tefon BE, Yildirim V, Ozcengiz E, Becher D, Hecker M, Ozcengiz

  1. Immunoproteomic analysis of Bordetella pertussis and identification of new

immunogenic proteins. Vaccine. 2009 Jan 22;27(4):542-8. doi:

10.1016/j.vaccine.2008.11.020. Epub 2008 Nov 24. PubMed PMID: 19028538.


Hecker M, Antelmann H, Büttner K, Bernhardt J. Gel-based proteomics of

Gram-positive bacteria: a powerful tool to address physiological questions.

Proteomics. 2008 Dec;8(23-24):4958-75. doi: 10.1002/pmic.200800278. Review.

PubMed PMID: 19003856.


Voigt B, Antelmann H, Albrecht D, Ehrenreich A, Maurer KH, Evers S,

Gottschalk G, van Dijl JM, Schweder T, Hecker M. Cell physiology and protein

secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol

Microbiol Biotechnol. 2009;16(1-2):53-68. doi: 10.1159/000142894. Epub 2008 Oct

  1. PubMed PMID: 18957862.


Eichstaedt S, Gäbler K, Below S, Müller C, Kohler C, Engelmann S,

Hildebrandt P, Völker U, Hecker M, Hildebrandt JP. Effects of Staphylococcus

aureus-hemolysin A on calcium signalling in immortalized human airway epithelial

cells. Cell Calcium. 2009 Feb;45(2):165-76. doi: 10.1016/j.ceca.2008.09.001. Epub

2008 Oct 15. PubMed PMID: 18922576.


Nagy G, Palkovics T, Otto A, Kusch H, Kocsis B, Dobrindt U, Engelmann S,

Hecker M, Emödy L, Pál T, Hacker J. “Gently rough”: the vaccine potential of a

Salmonella enterica regulatory lipopolysaccharide mutant. J Infect Dis. 2008 Dec

1;198(11):1699-706. doi: 10.1086/593069. PubMed PMID: 18922095.


Schlag S, Fuchs S, Nerz C, Gaupp R, Engelmann S, Liebeke M, Lalk M, Hecker

M, Götz F. Characterization of the oxygen-responsive NreABC regulon of

Staphylococcus aureus. J Bacteriol. 2008 Dec;190(23):7847-58. doi:

10.1128/JB.00905-08. Epub 2008 Sep 26. PubMed PMID: 18820014; PubMed Central

PMCID: PMC2583599.


Grumann D, Scharf SS, Holtfreter S, Kohler C, Steil L, Engelmann S, Hecker

M, Völker U, Bröker BM. Immune cell activation by enterotoxin gene cluster

(egc)-encoded and non-egc superantigens from Staphylococcus aureus. J Immunol.

2008 Oct 1;181(7):5054-61. PubMed PMID: 18802109.


Hahne H, Wolff S, Hecker M, Becher D. From complementarity to

comprehensiveness–targeting the membrane proteome of growing Bacillus subtilis

by divergent approaches. Proteomics. 2008 Oct;8(19):4123-36. doi:

10.1002/pmic.200800258. PubMed PMID: 18763711.


Kohler C, von Eiff C, Liebeke M, McNamara PJ, Lalk M, Proctor RA, Hecker M,

Engelmann S. A defect in menadione biosynthesis induces global changes in gene

expression in Staphylococcus aureus. J Bacteriol. 2008 Oct;190(19):6351-64. doi:

10.1128/JB.00505-08. Epub 2008 Aug 1. PubMed PMID: 18676673; PubMed Central

PMCID: PMC2566006.


Liebeke M, Pöther DC, van Duy N, Albrecht D, Becher D, Hochgräfe F, Lalk M,

Hecker M, Antelmann H. Depletion of thiol-containing proteins in response to

quinones in Bacillus subtilis. Mol Microbiol. 2008 Sep;69(6):1513-29. doi:

10.1111/j.1365-2958.2008.06382.x. Epub 2008 Jul 30. PubMed PMID: 18673455.


Reder A, Höper D, Weinberg C, Gerth U, Fraunholz M, Hecker M. The Spx

paralogue MgsR (YqgZ) controls a subregulon within the general stress response of

Bacillus subtilis. Mol Microbiol. 2008 Sep;69(5):1104-20. doi:

10.1111/j.1365-2958.2008.06332.x. Epub 2008 Jul 14. PubMed PMID: 18643936.


Wolf C, Hochgräfe F, Kusch H, Albrecht D, Hecker M, Engelmann S. Proteomic

analysis of antioxidant strategies of Staphylococcus aureus: diverse responses to

different oxidants. Proteomics. 2008 Aug;8(15):3139-53. doi:

10.1002/pmic.200701062. PubMed PMID: 18604844.


Mostertz J, Hochgräfe F, Jürgen B, Schweder T, Hecker M. The role of

thioredoxin TrxA in Bacillus subtilis: a proteomics and transcriptomics approach.

Proteomics. 2008 Jul;8(13):2676-90. doi: 10.1002/pmic.200701015. PubMed PMID:



Westers L, Westers H, Zanen G, Antelmann H, Hecker M, Noone D, Devine KM,

van Dijl JM, Quax WJ. Genetic or chemical protease inhibition causes significant

changes in the Bacillus subtilis exoproteome. Proteomics. 2008 Jul;8(13):2704-13.

doi: 10.1002/pmic.200800009. PubMed PMID: 18546160.


Raberg M, Reinecke F, Reichelt R, Malkus U, König S, Pötter M, Fricke WF,

Pohlmann A, Voigt B, Hecker M, Friedrich B, Bowien B, Steinbüchel A. Ralstonia

eutropha H16 flagellation changes according to nutrient supply and state of

poly(3-hydroxybutyrate) accumulation. Appl Environ Microbiol. 2008

Jul;74(14):4477-90. doi: 10.1128/AEM.00440-08. Epub 2008 May 23. PubMed PMID:

18502919; PubMed Central PMCID: PMC2493158.


Schweder T, Markert S, Hecker M. Proteomics of marine bacteria.

Electrophoresis. 2008 Jun;29(12):2603-16. doi: 10.1002/elps.200800009. Review.

PubMed PMID: 18494036.


Dreisbach A, Otto A, Becher D, Hammer E, Teumer A, Gouw JW, Hecker M, Völker

  1. Monitoring of changes in the membrane proteome during stationary phase

adaptation of Bacillus subtilis using in vivo labeling techniques. Proteomics.

2008 May;8(10):2062-76. doi: 10.1002/pmic.200701081. PubMed PMID: 18491319.


Hochgräfe F, Wolf C, Fuchs S, Liebeke M, Lalk M, Engelmann S, Hecker M.

Nitric oxide stress induces different responses but mediates comparable protein

thiol protection in Bacillus subtilis and Staphylococcus aureus. J Bacteriol.

2008 Jul;190(14):4997-5008. doi: 10.1128/JB.01846-07. Epub 2008 May 16. PubMed

PMID: 18487332; PubMed Central PMCID: PMC2447014.


Wolff S, Hahne H, Hecker M, Becher D. Complementary analysis of the

vegetative membrane proteome of the human pathogen Staphylococcus aureus. Mol

Cell Proteomics. 2008 Aug;7(8):1460-8. doi: 10.1074/mcp.M700554-MCP200. Epub 2008

May 6. PubMed PMID: 18460691; PubMed Central PMCID: PMC2500231.


Raddatz K, Albrecht D, Hochgräfe F, Hecker M, Gotthardt M. A proteome map of

murine heart and skeletal muscle. Proteomics. 2008 May;8(9):1885-97. doi:

10.1002/pmic.200700902. PubMed PMID: 18398877.


Zweers JC, Barák I, Becher D, Driessen AJ, Hecker M, Kontinen VP, Saller MJ,

Vavrová L, van Dijl JM. Towards the development of Bacillus subtilis as a cell

factory for membrane proteins and protein complexes. Microb Cell Fact. 2008 Apr

4;7:10. doi: 10.1186/1475-2859-7-10. PubMed PMID: 18394159; PubMed Central PMCID:



Hieu CX, Voigt B, Albrecht D, Becher D, Lombardot T, Glöckner FO, Amann R,

Hecker M, Schweder T. Detailed proteome analysis of growing cells of the

planctomycete Rhodopirellula baltica SH1T. Proteomics. 2008 Apr;8(8):1608-23.

doi: 10.1002/pmic.200701017. PubMed PMID: 18340632.


Lorenz U, Hüttinger C, Schäfer T, Ziebuhr W, Thiede A, Hacker J, Engelmann

S, Hecker M, Ohlsen K. The alternative sigma factor sigma B of Staphylococcus

aureus modulates virulence in experimental central venous catheter-related

infections. Microbes Infect. 2008 Mar;10(3):217-23. doi:

10.1016/j.micinf.2007.11.006. Epub 2007 Nov 28. PubMed PMID: 18328762.


Engelmann S, Hecker M. Proteomic analysis to investigate regulatory networks

in Staphylococcus aureus. Methods Mol Biol. 2008;431:25-45. PubMed PMID:



Antelmann H, Hecker M, Zuber P. Proteomic signatures uncover thiol-specific

electrophile resistance mechanisms in Bacillus subtilis. Expert Rev Proteomics.

2008 Feb;5(1):77-90. doi: 10.1586/14789450.5.1.77. Review. PubMed PMID: 18282125.


Galka F, Wai SN, Kusch H, Engelmann S, Hecker M, Schmeck B, Hippenstiel S,

Uhlin BE, Steinert M. Proteomic characterization of the whole secretome of

Legionella pneumophila and functional analysis of outer membrane vesicles. Infect

Immun. 2008 May;76(5):1825-36. doi: 10.1128/IAI.01396-07. Epub 2008 Feb 4. PubMed

PMID: 18250176; PubMed Central PMCID: PMC2346698.


Pioch D, Jürgen B, Evers S, Maurer KH, Hecker M, Schweder T. Improved

sandwich-hybridization assay for an electrical DNA-chip-based monitoring of

bioprocess-relevant marker genes. Appl Microbiol Biotechnol. 2008

Mar;78(4):719-28. doi: 10.1007/s00253-008-1347-z. Epub 2008 Jan 24. PubMed PMID:



Leelakriangsak M, Huyen NT, Töwe S, van Duy N, Becher D, Hecker M, Antelmann

H, Zuber P. Regulation of quinone detoxification by the thiol stress sensing

DUF24/MarR-like repressor, YodB in Bacillus subtilis. Mol Microbiol. 2008

Mar;67(5):1108-24. doi: 10.1111/j.1365-2958.2008.06110.x. Epub 2008 Jan 16.

PubMed PMID: 18208493.


Hecker M, Pané-Farré J, Völker U. SigB-dependent general stress response in

Bacillus subtilis and related gram-positive bacteria. Annu Rev Microbiol.

2007;61:215-36. Review. PubMed PMID: 18035607.


Gerth U, Kock H, Kusters I, Michalik S, Switzer RL, Hecker M. Clp-dependent

proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus

subtilis. J Bacteriol. 2008 Jan;190(1):321-31. Epub 2007 Nov 2. PubMed PMID:

17981983; PubMed Central PMCID: PMC2223743.


Eymann C, Becher D, Bernhardt J, Gronau K, Klutzny A, Hecker M. Dynamics of

protein phosphorylation on Ser/Thr/Tyr in Bacillus subtilis. Proteomics. 2007

Oct;7(19):3509-26. PubMed PMID: 17726680.


Schwarz R, Liang C, Kaleta C, Kühnel M, Hoffmann E, Kuznetsov S, Hecker M,

Griffiths G, Schuster S, Dandekar T. Integrated network reconstruction,

visualization and analysis using YANAsquare. BMC Bioinformatics. 2007 Aug

28;8:313. PubMed PMID: 17725829; PubMed Central PMCID: PMC2020486.


Töwe S, Leelakriangsak M, Kobayashi K, Van Duy N, Hecker M, Zuber P,

Antelmann H. The MarR-type repressor MhqR (YkvE) regulates multiple

dioxygenases/glyoxalases and an azoreductase which confer resistance to

2-methylhydroquinone and catechol in Bacillus subtilis. Mol Microbiol. 2007

Oct;66(1):40-54. Epub 2007 Aug 27. PubMed PMID: 17725564.


De Buck E, Höper D, Lammertyn E, Hecker M, Anné J. Differential 2-D protein

gel electrophoresis analysis of Legionella pneumophila wild type and Tat

secretion mutants. Int J Med Microbiol. 2008 Jul;298(5-6):449-61. Epub 2007 Aug

  1. PubMed PMID: 17723319.


Hochgräfe F, Mostertz J, Pöther DC, Becher D, Helmann JD, Hecker M.

S-cysteinylation is a general mechanism for thiol protection of Bacillus subtilis

proteins after oxidative stress. J Biol Chem. 2007 Sep 7;282(36):25981-5. Epub

2007 Jul 4. PubMed PMID: 17611193.


Hyyryläinen HL, Pietiäinen M, Lundén T, Ekman A, Gardemeister M,

Murtomäki-Repo S, Antelmann H, Hecker M, Valmu L, Sarvas M, Kontinen VP. The

density of negative charge in the cell wall influences two-component signal

transduction in Bacillus subtilis. Microbiology. 2007 Jul;153(Pt 7):2126-36.

PubMed PMID: 17600057.


Brüsehaber E, Böttcher D, Musidlowska-Persson A, Albrecht D, Hecker M,

Doderer K, Bornscheuer UT. Identification of pig liver esterase variants by

tandem mass spectroscopy analysis and their characterization. Appl Microbiol

Biotechnol. 2007 Sep;76(4):853-9. Epub 2007 Jun 26. PubMed PMID: 17593363.


Kusch H, Engelmann S, Bode R, Albrecht D, Morschhäuser J, Hecker M. A

proteomic view of Candida albicans yeast cell metabolism in exponential and

stationary growth phases. Int J Med Microbiol. 2008 Apr;298(3-4):291-318. Epub

2007 Jun 27. PubMed PMID: 17588813.


Halbedel S, Eilers H, Jonas B, Busse J, Hecker M, Engelmann S, Stülke J.

Transcription in Mycoplasma pneumoniae: analysis of the promoters of the ackA and

ldh genes. J Mol Biol. 2007 Aug 17;371(3):596-607. Epub 2007 Jun 9. PubMed PMID:



Becker K, Bierbaum G, von Eiff C, Engelmann S, Götz F, Hacker J, Hecker M,

Peters G, Rosenstein R, Ziebuhr W. Understanding the physiology and adaptation of

staphylococci: a post-genomic approach. Int J Med Microbiol. 2007

Nov;297(7-8):483-501. Epub 2007 Jun 19. Review. PubMed PMID: 17581783.


Nguyen VD, Wolf C, Mäder U, Lalk M, Langer P, Lindequist U, Hecker M,

Antelmann H. Transcriptome and proteome analyses in response to

2-methylhydroquinone and 6-brom-2-vinyl-chroman-4-on reveal different degradation

systems involved in the catabolism of aromatic compounds in Bacillus subtilis.

Proteomics. 2007 May;7(9):1391-408. PubMed PMID: 17407181.


Luo Y, Vilain S, Voigt B, Albrecht D, Hecker M, Brözel VS. Proteomic

analysis of Bacillus cereus growing in liquid soil organic matter. FEMS Microbiol

Lett. 2007 Jun;271(1):40-7. Epub 2007 Mar 28. PubMed PMID: 17391366.


Fuchs S, Pané-Farré J, Kohler C, Hecker M, Engelmann S. Anaerobic gene

expression in Staphylococcus aureus. J Bacteriol. 2007 Jun;189(11):4275-89. Epub

2007 Mar 23. PubMed PMID: 17384184; PubMed Central PMCID: PMC1913399.


Kirstein J, Dougan DA, Gerth U, Hecker M, Turgay K. The tyrosine kinase McsB

is a regulated adaptor protein for ClpCP. EMBO J. 2007 Apr 18;26(8):2061-70. Epub

2007 Mar 22. PubMed PMID: 17380125; PubMed Central PMCID: PMC1852781.


Ozcan S, Yildirim V, Kaya L, Albrecht D, Becher D, Hecker M, Ozcengiz G.

Phanerochaete chrysosporium soluble proteome as a prelude for the analysis of

heavy metal stress response. Proteomics. 2007 Apr;7(8):1249-60. PubMed PMID:



Hardwick SW, Pané-Farré J, Delumeau O, Marles-Wright J, Murray JW, Hecker M,

Lewis RJ. Structural and functional characterization of partner switching

regulating the environmental stress response in Bacillus subtilis. J Biol Chem.

2007 Apr 13;282(15):11562-72. Epub 2007 Feb 15. PubMed PMID: 17303566.


Duy NV, Mäder U, Tran NP, Cavin JF, Tam le T, Albrecht D, Hecker M,

Antelmann H. The proteome and transcriptome analysis of Bacillus subtilis in

response to salicylic acid. Proteomics. 2007 Mar;7(5):698-710. PubMed PMID:



Kusch H, Engelmann S, Albrecht D, Morschhäuser J, Hecker M. Proteomic

analysis of the oxidative stress response in Candida albicans. Proteomics. 2007

Mar;7(5):686-97. PubMed PMID: 17285563.


Voigt B, Hoi le T, Jürgen B, Albrecht D, Ehrenreich A, Veith B, Evers S,

Maurer KH, Hecker M, Schweder T. The glucose and nitrogen starvation response of

Bacillus licheniformis. Proteomics. 2007 Feb;7(3):413-23. PubMed PMID: 17274076.


Antelmann H, Töwe S, Albrecht D, Hecker M. The phosphorus source phytate

changes the composition of the cell wall proteome in Bacillus subtilis. J

Proteome Res. 2007 Feb;6(2):897-903. PubMed PMID: 17269748.


Markert S, Arndt C, Felbeck H, Becher D, Sievert SM, Hügler M, Albrecht D,

Robidart J, Bench S, Feldman RA, Hecker M, Schweder T. Physiological proteomics

of the uncultured endosymbiont of Riftia pachyptila. Science. 2007 Jan

12;315(5809):247-50. PubMed PMID: 17218528.


Bandow JE, Hecker M. Proteomic profiling of cellular stresses in Bacillus

subtilis reveals cellular networks and assists in elucidating antibiotic

mechanisms of action. Prog Drug Res. 2007;64:79, 81-101. Review. PubMed PMID:


Tam le T, Eymann C, Antelmann H, Albrecht D, Hecker M. Global gene

expression profiling of Bacillus subtilis in response to ammonium and tryptophan

starvation as revealed by transcriptome and proteome analysis. J Mol Microbiol

Biotechnol. 2007;12(1-2):121-30. PubMed PMID: 17183219.


Darmon E, Dorenbos R, Meens J, Freudl R, Antelmann H, Hecker M, Kuipers OP,

Bron S, Quax WJ, Dubois JY, van Dijl JM. A disulfide bond-containing alkaline

phosphatase triggers a BdbC-dependent secretion stress response in Bacillus

subtilis. Appl Environ Microbiol. 2006 Nov;72(11):6876-85. PubMed PMID: 17088376;

PubMed Central PMCID: PMC1636209.


Rogasch K, Rühmling V, Pané-Farré J, Höper D, Weinberg C, Fuchs S, Schmudde

M, Bröker BM, Wolz C, Hecker M, Engelmann S. Influence of the two-component

system SaeRS on global gene expression in two different Staphylococcus aureus

strains. J Bacteriol. 2006 Nov;188(22):7742-58. PubMed PMID: 17079681; PubMed

Central PMCID: PMC1636327.


Wolff S, Antelmann H, Albrecht D, Becher D, Bernhardt J, Bron S, Büttner K,

van Dijl JM, Eymann C, Otto A, Tam le T, Hecker M. Towards the entire proteome of

the model bacterium Bacillus subtilis by gel-based and gel-free approaches. J

Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 15;849(1-2):129-40. Epub

2006 Oct 20. Review. PubMed PMID: 17055787.


Sibbald MJ, Ziebandt AK, Engelmann S, Hecker M, de Jong A, Harmsen HJ,

Raangs GC, Stokroos I, Arends JP, Dubois JY, van Dijl JM. Mapping the pathways to

staphylococcal pathogenesis by comparative secretomics. Microbiol Mol Biol Rev.

2006 Sep;70(3):755-88. Review. PubMed PMID: 16959968; PubMed Central PMCID:



Silbersack J, Jürgen B, Hecker M, Schneidinger B, Schmuck R, Schweder T. An

acetoin-regulated expression system of Bacillus subtilis. Appl Microbiol

Biotechnol. 2006 Dec;73(4):895-903. Epub 2006 Aug 31. PubMed PMID: 16944132.


Antelmann H, Van Dijl JM, Bron S, Hecker M. Proteomic survey through

secretome of Bacillus subtilis. Methods Biochem Anal. 2006;49:179-208. Review.

PubMed PMID: 16929680.


Tam le T, Eymann C, Albrecht D, Sietmann R, Schauer F, Hecker M, Antelmann

  1. Differential gene expression in response to phenol and catechol reveals

different metabolic activities for the degradation of aromatic compounds in

Bacillus subtilis. Environ Microbiol. 2006 Aug;8(8):1408-27. PubMed PMID:



Lioy VS, Martín MT, Camacho AG, Lurz R, Antelmann H, Hecker M, Hitchin E,

Ridge Y, Wells JM, Alonso JC. pSM19035-encoded zeta toxin induces stasis followed

by death in a subpopulation of cells. Microbiology. 2006 Aug;152(Pt 8):2365-79.

PubMed PMID: 16849801.


Tam le T, Antelmann H, Eymann C, Albrecht D, Bernhardt J, Hecker M. Proteome

signatures for stress and starvation in Bacillus subtilis as revealed by a 2-D

gel image color coding approach. Proteomics. 2006 Aug;6(16):4565-85. PubMed PMID:



Pamp SJ, Frees D, Engelmann S, Hecker M, Ingmer H. Spx is a global effector

impacting stress tolerance and biofilm formation in Staphylococcus aureus. J

Bacteriol. 2006 Jul;188(13):4861-70. PubMed PMID: 16788195; PubMed Central PMCID:



Miethke M, Hecker M, Gerth U. Involvement of Bacillus subtilis ClpE in CtsR

degradation and protein quality control. J Bacteriol. 2006 Jul;188(13):4610-9.

PubMed PMID: 16788169; PubMed Central PMCID: PMC1482982.


Meyer zu Schwabedissen HE, Dreisbach A, Hammer E, Fusch C, Hecker M, Völker

U, Kroemer HK. Direct mass spectrometric identification of ABCB1

(P-glycoprotein/MDR1) from the apical membrane fraction of human placenta using

fourier transform ion cyclotron mass spectrometry. Pharmacogenet Genomics. 2006

Jun;16(6):385-9. PubMed PMID: 16708047.


Hoi le T, Voigt B, Jürgen B, Ehrenreich A, Gottschalk G, Evers S, Feesche J,

Maurer KH, Hecker M, Schweder T. The phosphate-starvation response of Bacillus

licheniformis. Proteomics. 2006 Jun;6(12):3582-601. PubMed PMID: 16705752.


Zanen G, Antelmann H, Meima R, Jongbloed JD, Kolkman M, Hecker M, van Dijl

JM, Quax WJ. Proteomic dissection of potential signal recognition particle

dependence in protein secretion by Bacillus subtilis. Proteomics. 2006

Jun;6(12):3636-48. PubMed PMID: 16705751.


Batzilla CF, Rachid S, Engelmann S, Hecker M, Hacker J, Ziebuhr W. Impact of

the accessory gene regulatory system (Agr) on extracellular proteins, codY

expression and amino acid metabolism in Staphylococcus epidermidis. Proteomics.

2006 Jun;6(12):3602-13. PubMed PMID: 16691552.


Ollinger J, Song KB, Antelmann H, Hecker M, Helmann JD. Role of the Fur

regulon in iron transport in Bacillus subtilis. J Bacteriol. 2006

May;188(10):3664-73. PubMed PMID: 16672620; PubMed Central PMCID: PMC1482855.


Pané-Farré J, Jonas B, Förstner K, Engelmann S, Hecker M. The sigmaB regulon

in Staphylococcus aureus and its regulation. Int J Med Microbiol. 2006

Aug;296(4-5):237-58. Epub 2006 Apr 27. PubMed PMID: 16644280.


Holtfreter S, Roschack K, Eichler P, Eske K, Holtfreter B, Kohler C,

Engelmann S, Hecker M, Greinacher A, Broker BM. Staphylococcus aureus carriers

neutralize superantigens by antibodies specific for their colonizing strain: a

potential explanation for their improved prognosis in severe sepsis. J Infect

Dis. 2006 May 1;193(9):1275-8. Epub 2006 Mar 23. PubMed PMID: 16586365.


Wolff S, Otto A, Albrecht D, Zeng JS, Büttner K, Glückmann M, Hecker M,

Becher D. Gel-free and gel-based proteomics in Bacillus subtilis: a comparative

study. Mol Cell Proteomics. 2006 Jul;5(7):1183-92. Epub 2006 Mar 21. PubMed PMID:



Höper D, Bernhardt J, Hecker M. Salt stress adaptation of Bacillus subtilis:

a physiological proteomics approach. Proteomics. 2006 Mar;6(5):1550-62. PubMed

PMID: 16440371.

2005 - 2000

Kohler C, Wolff S, Albrecht D, Fuchs S, Becher D, Büttner K, Engelmann S,

Hecker M. Proteome analyses of Staphylococcus aureus in growing and non-growing

cells: a physiological approach. Int J Med Microbiol. 2005 Dec;295(8):547-65.

Epub 2005 Oct 25. PubMed PMID: 16325551.


Voigt B, Schweder T, Sibbald MJ, Albrecht D, Ehrenreich A, Bernhardt J,

Feesche J, Maurer KH, Gottschalk G, van Dijl JM, Hecker M. The extracellular

proteome of Bacillus licheniformis grown in different media and under different

nutrient starvation conditions. Proteomics. 2006 Jan;6(1):268-81. Erratum in:

Proteomics. 2006 Mar;6(5):1704-5. PubMed PMID: 16317772.


Hochgräfe F, Mostertz J, Albrecht D, Hecker M. Fluorescence thiol

modification assay: oxidatively modified proteins in Bacillus subtilis. Mol

Microbiol. 2005 Oct;58(2):409-25. PubMed PMID: 16194229.


Jürgen B, Barken KB, Tobisch S, Pioch D, Wümpelmann M, Hecker M, Schweder T.

Application of an electric DNA-chip for the expression analysis of

bioprocess-relevant marker genes of Bacillus subtilis. Biotechnol Bioeng. 2005

Nov 5;92(3):299-307. PubMed PMID: 16180240.


Jürgen B, Tobisch S, Wümpelmann M, Gördes D, Koch A, Thurow K, Albrecht D,

Hecker M, Schweder T. Global expression profiling of Bacillus subtilis cells

during industrial-close fed-batch fermentations with different nitrogen sources.

Biotechnol Bioeng. 2005 Nov 5;92(3):277-98. PubMed PMID: 16178035.


Kirstein J, Zühlke D, Gerth U, Turgay K, Hecker M. A tyrosine kinase and its

activator control the activity of the CtsR heat shock repressor in B. subtilis.

EMBO J. 2005 Oct 5;24(19):3435-45. Epub 2005 Sep 15. PubMed PMID: 16163393;

PubMed Central PMCID: PMC1276163.


Antelmann H, Williams RC, Miethke M, Wipat A, Albrecht D, Harwood CR, Hecker

  1. The extracellular and cytoplasmic proteomes of the non-virulent Bacillus

anthracis strain UM23C1-2. Proteomics. 2005 Sep;5(14):3684-95. PubMed PMID:



Völker U, Hecker M. From genomics via proteomics to cellular physiology of

the Gram-positive model organism Bacillus subtilis. Cell Microbiol. 2005

Aug;7(8):1077-85. Review. PubMed PMID: 16008575.


Westers H, Braun PG, Westers L, Antelmann H, Hecker M, Jongbloed JD,

Yoshikawa H, Tanaka T, van Dijl JM, Quax WJ. Genes involved in SkfA killing

factor production protect a Bacillus subtilis lipase against proteolysis. Appl

Environ Microbiol. 2005 Apr;71(4):1899-908. PubMed PMID: 15812018; PubMed Central

PMCID: PMC1082511.


Koburger T, Weibezahn J, Bernhardt J, Homuth G, Hecker M. Genome-wide mRNA

profiling in glucose starved Bacillus subtilis cells. Mol Genet Genomics. 2005

Aug;274(1):1-12. Epub 2005 Apr 5. PubMed PMID: 15809868.


Höper D, Völker U, Hecker M. Comprehensive characterization of the

contribution of individual SigB-dependent general stress genes to stress

resistance of Bacillus subtilis. J Bacteriol. 2005 Apr;187(8):2810-26. PubMed

PMID: 15805528; PubMed Central PMCID: PMC1070366.


Rosen R, Becher D, Büttner K, Biran D, Hecker M, Ron EZ. Probing the active

site of homoserine trans-succinylase. FEBS Lett. 2004 Nov 19;577(3):386-92.

PubMed PMID: 15556615.


Jongbloed JD, Grieger U, Antelmann H, Hecker M, Nijland R, Bron S, van Dijl

  1. Two minimal Tat translocases in Bacillus. Mol Microbiol. 2004

Dec;54(5):1319-25. PubMed PMID: 15554971.


Hecker M, Völker U. Towards a comprehensive understanding of Bacillus

subtilis cell physiology by physiological proteomics. Proteomics. 2004

Dec;4(12):3727-50. Review. PubMed PMID: 15540212.


Rosen R, Becher D, Büttner K, Biran D, Hecker M, Ron EZ. Highly

phosphorylated bacterial proteins. Proteomics. 2004 Oct;4(10):3068-77. PubMed

PMID: 15378765.


Eymann C, Dreisbach A, Albrecht D, Bernhardt J, Becher D, Gentner S, Tam le

T, Büttner K, Buurman G, Scharf C, Venz S, Völker U, Hecker M. A comprehensive

proteome map of growing Bacillus subtilis cells. Proteomics. 2004

Oct;4(10):2849-76. PubMed PMID: 15378759.


Ziebandt AK, Becher D, Ohlsen K, Hacker J, Hecker M, Engelmann S. The

influence of agr and sigmaB in growth phase dependent regulation of virulence

factors in Staphylococcus aureus. Proteomics. 2004 Oct;4(10):3034-47. PubMed

PMID: 15378746.


Zanen G, Antelmann H, Westers H, Hecker M, van Dijl JM, Quax WJ. FlhF, the

third signal recognition particle-GTPase of Bacillus subtilis, is dispensable for

protein secretion. J Bacteriol. 2004 Sep;186(17):5956-60. PubMed PMID: 15317803;

PubMed Central PMCID: PMC516824.


Kock H, Gerth U, Hecker M. The ClpP peptidase is the major determinant of

bulk protein turnover in Bacillus subtilis. J Bacteriol. 2004

Sep;186(17):5856-64. PubMed PMID: 15317791; PubMed Central PMCID: PMC516825.


Allenby NE, O’Connor N, Prágai Z, Carter NM, Miethke M, Engelmann S, Hecker

M, Wipat A, Ward AC, Harwood CR. Post-transcriptional regulation of the Bacillus

subtilis pst operon encoding a phosphate-specific ABC transporter. Microbiology.

2004 Aug;150(Pt 8):2619-28. PubMed PMID: 15289558.


Antelmann H, Sapolsky R, Miller B, Ferrari E, Chotani G, Weyler W, Gaertner

A, Hecker M. Quantitative proteome profiling during the fermentation process of

pleiotropic Bacillus subtilis mutants. Proteomics. 2004 Aug;4(8):2408-24. PubMed

PMID: 15274136.


Schweder T, Hecker M. Monitoring of stress responses. Adv Biochem Eng

Biotechnol. 2004;89:47-71. Review. PubMed PMID: 15217155.


Voigt B, Schweder T, Becher D, Ehrenreich A, Gottschalk G, Feesche J, Maurer

KH, Hecker M. A proteomic view of cell physiology of Bacillus licheniformis.

Proteomics. 2004 May;4(5):1465-90. PubMed PMID: 15188415.


Tjalsma H, Antelmann H, Jongbloed JD, Braun PG, Darmon E, Dorenbos R, Dubois

JY, Westers H, Zanen G, Quax WJ, Kuipers OP, Bron S, Hecker M, van Dijl JM.

Proteomics of protein secretion by Bacillus subtilis: separating the “secrets” of

the secretome. Microbiol Mol Biol Rev. 2004 Jun;68(2):207-33. Review. PubMed

PMID: 15187182; PubMed Central PMCID: PMC419921.


Kusch H, Biswas K, Schwanfelder S, Engelmann S, Rogers PD, Hecker M,

Morschhäuser J. A proteomic approach to understanding the development of

multidrug-resistant Candida albicans strains. Mol Genet Genomics. 2004

Jun;271(5):554-65. Epub 2004 Apr 28. PubMed PMID: 15114480.


Mäder U, Hennig S, Hecker M, Homuth G. Transcriptional organization and

posttranscriptional regulation of the Bacillus subtilis branched-chain amino acid

biosynthesis genes. J Bacteriol. 2004 Apr;186(8):2240-52. PubMed PMID: 15060025;

PubMed Central PMCID: PMC412147.


Weber H, Engelmann S, Becher D, Hecker M. Oxidative stress triggers thiol

oxidation in the glyceraldehyde-3-phosphate dehydrogenase of Staphylococcus

aureus. Mol Microbiol. 2004 Apr;52(1):133-40. PubMed PMID: 15049816.


Rosen R, Sacher A, Shechter N, Becher D, Büttner K, Biran D, Hecker M, Ron EZ,

Two-dimensional reference map of Agrobacterium tumefaciens proteins.

Proteomics. 2004 Apr;4(4):1061-73. PubMed PMID: 15048987.


Vitikainen M, Lappalainen I, Seppala R, Antelmann H, Boer H, Taira S,

Savilahti H, Hecker M, Vihinen M, Sarvas M, Kontinen VP. Structure-function

analysis of PrsA reveals roles for the parvulin-like and flanking N- and

C-terminal domains in protein folding and secretion in Bacillus subtilis. J Biol

Chem. 2004 Apr 30;279(18):19302-14. Epub 2004 Feb 19. PubMed PMID: 14976191.


Sender U, Bandow J, Engelmann S, Lindequist U, Hecker M. Proteomic

signatures for daunomycin and adriamycin in Bacillus subtilis. Pharmazie. 2004

Jan;59(1):65-70. PubMed PMID: 14964425.


Mostertz J, Scharf C, Hecker M, Homuth G. Transcriptome and proteome

analysis of Bacillus subtilis gene expression in response to superoxide and

peroxide stress. Microbiology. 2004 Feb;150(Pt 2):497-512. PubMed PMID: 14766928.


Kock H, Gerth U, Hecker M. MurAA, catalysing the first committed step in

peptidoglycan biosynthesis, is a target of Clp-dependent proteolysis in Bacillus

subtilis. Mol Microbiol. 2004 Feb;51(4):1087-102. PubMed PMID: 14763982.


Gerth U, Kirstein J, Mostertz J, Waldminghaus T, Miethke M, Kock H, Hecker M,

Fine-tuning in regulation of Clp protein content in Bacillus subtilis. J

Bacteriol. 2004 Jan;186(1):179-91. PubMed PMID: 14679237; PubMed Central PMCID:



Kohler C, von Eiff C, Peters G, Proctor RA, Hecker M, Engelmann S.

Physiological characterization of a heme-deficient mutant of Staphylococcus

aureus by a proteomic approach. J Bacteriol. 2003 Dec;185(23):6928-37. PubMed

PMID: 14617657; PubMed Central PMCID: PMC262702.


Westers H, Dorenbos R, van Dijl JM, Kabel J, Flanagan T, Devine KM, Jude F,

Seror SJ, Beekman AC, Darmon E, Eschevins C, de Jong A, Bron S, Kuipers OP,

Albertini AM, Antelmann H, Hecker M, Zamboni N, Sauer U, Bruand C, Ehrlich DS,

Alonso JC, Salas M, Quax WJ. Genome engineering reveals large dispensable regions

in Bacillus subtilis. Mol Biol Evol. 2003 Dec;20(12):2076-90. Epub 2003 Aug 29.

PubMed PMID: 12949151.


Hecker M. A proteomic view of cell physiology of Bacillus subtilis–bringing

the genome sequence to life. Adv Biochem Eng Biotechnol. 2003;83:57-92. Review.

PubMed PMID: 12934926.


Luhn S, Berth M, Hecker M, Bernhardt J. Using standard positions and image

fusion to create proteome maps from collections of two-dimensional gel

electrophoresis images. Proteomics. 2003 Jul;3(7):1117-27. PubMed PMID: 12872213.


Blencke HM, Homuth G, Ludwig H, Mäder U, Hecker M, Stülke J. Transcriptional

profiling of gene expression in response to glucose in Bacillus subtilis:

regulation of the central metabolic pathways. Metab Eng. 2003 Apr;5(2):133-49.

PubMed PMID: 12850135.


Mostertz J, Hecker M. Patterns of protein carbonylation following oxidative

stress in wild-type and sigB Bacillus subtilis cells. Mol Genet Genomics. 2003

Aug;269(5):640-8. Epub 2003 Jul 4. PubMed PMID: 12845527.


Antelmann H, Darmon E, Noone D, Veening JW, Westers H, Bron S, Kuipers OP,

Devine KM, Hecker M, van Dijl JM. The extracellular proteome of Bacillus subtilis

under secretion stress conditions. Mol Microbiol. 2003 Jul;49(1):143-56. PubMed

PMID: 12823817.


Rosen R, Matthysse AG, Becher D, Biran D, Yura T, Hecker M, Ron EZ. Proteome

analysis of plant-induced proteins of Agrobacterium tumefaciens. FEMS Microbiol

Ecol. 2003 Jun 1;44(3):355-60. doi: 10.1016/S0168-6496(03)00077-1. PubMed PMID:



Kobayashi K, Ehrlich SD, Albertini A, Amati G, Andersen KK, Arnaud M, Asai

K, Ashikaga S, Aymerich S, Bessieres P, Boland F, Brignell SC, Bron S, Bunai K,

Chapuis J, Christiansen LC, Danchin A, Débarbouille M, Dervyn E, Deuerling E,

Devine K, Devine SK, Dreesen O, Errington J, Fillinger S, Foster SJ, Fujita Y,

Galizzi A, Gardan R, Eschevins C, Fukushima T, Haga K, Harwood CR, Hecker M,

Hosoya D, Hullo MF, Kakeshita H, Karamata D, Kasahara Y, Kawamura F, Koga K,

Koski P, Kuwana R, Imamura D, Ishimaru M, Ishikawa S, Ishio I, Le Coq D, Masson

A, Mauël C, Meima R, Mellado RP, Moir A, Moriya S, Nagakawa E, Nanamiya H, Nakai

S, Nygaard P, Ogura M, Ohanan T, O’Reilly M, O’Rourke M, Pragai Z, Pooley HM,

Rapoport G, Rawlins JP, Rivas LA, Rivolta C, Sadaie A, Sadaie Y, Sarvas M, Sato

T, Saxild HH, Scanlan E, Schumann W, Seegers JF, Sekiguchi J, Sekowska A, Séror

SJ, Simon M, Stragier P, Studer R, Takamatsu H, Tanaka T, Takeuchi M, Thomaides

HB, Vagner V, van Dijl JM, Watabe K, Wipat A, Yamamoto H, Yamamoto M, Yamamoto Y,

Yamane K, Yata K, Yoshida K, Yoshikawa H, Zuber U, Ogasawara N. Essential

Bacillus subtilis genes. Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4678-83.

Epub 2003 Apr 7. PubMed PMID: 12682299; PubMed Central PMCID: PMC153615.


Hecker M, Engelmann S, Cordwell SJ. Proteomics of Staphylococcus

aureus–current state and future challenges. J Chromatogr B Analyt Technol Biomed

Life Sci. 2003 Apr 5;787(1):179-95. Review. PubMed PMID: 12659740.


Bandow JE, Becher D, Büttner K, Hochgräfe F, Freiberg C, Brötz H, Hecker M.

The role of peptide deformylase in protein biosynthesis: a proteomic study.

Proteomics. 2003 Mar;3(3):299-306. PubMed PMID: 12627383.


Leichert LI, Scharf C, Hecker M. Global characterization of disulfide stress

in Bacillus subtilis. J Bacteriol. 2003 Mar;185(6):1967-75. PubMed PMID:

12618461; PubMed Central PMCID: PMC150141.


Bandow JE, Brötz H, Leichert LI, Labischinski H, Hecker M. Proteomic

approach to understanding antibiotic action. Antimicrob Agents Chemother. 2003

Mar;47(3):948-55. PubMed PMID: 12604526; PubMed Central PMCID: PMC149304.


Bernhardt J, Weibezahn J, Scharf C, Hecker M. Bacillus subtilis during feast

and famine: visualization of the overall regulation of protein synthesis during

glucose starvation by proteome analysis. Genome Res. 2003 Feb;13(2):224-37.

PubMed PMID: 12566400; PubMed Central PMCID: PMC420377.


Rollenhagen C, Antelmann H, Kirstein J, Delumeau O, Hecker M, Yudkin MD.

Binding of sigma(A) and sigma(B) to core RNA polymerase after environmental

stress in Bacillus subtilis. J Bacteriol. 2003 Jan;185(1):35-40. PubMed PMID:

12486038; PubMed Central PMCID: PMC141833.


Mäder U, Antelmann H, Buder T, Dahl MK, Hecker M, Homuth G. Bacillus

subtilis functional genomics: genome-wide analysis of the DegS-DegU regulon by

transcriptomics and proteomics. Mol Genet Genomics. 2002 Dec;268(4):455-67. Epub

2002 Nov 16. PubMed PMID: 12471443.


Jongbloed JD, Antelmann H, Hecker M, Nijland R, Bron S, Airaksinen U, Pries

F, Quax WJ, van Dijl JM, Braun PG. Selective contribution of the twin-arginine

translocation pathway to protein secretion in Bacillus subtilis. J Biol Chem.

2002 Nov 15;277(46):44068-78. Epub 2002 Sep 5. PubMed PMID: 12218047.


Lindner C, Hecker M, Le Coq D, Deutscher J. Bacillus subtilis mutant LicT

antiterminators exhibiting enzyme I- and HPr-independent antitermination affect

catabolite repression of the bglPH operon. J Bacteriol. 2002 Sep;184(17):4819-28.

PubMed PMID: 12169607; PubMed Central PMCID: PMC135283.


van Dijl JM, Braun PG, Robinson C, Quax WJ, Antelmann H, Hecker M, Müller J,

Tjalsma H, Bron S, Jongbloed JD. Functional genomic analysis of the Bacillus

subtilis Tat pathway for protein secretion. J Biotechnol. 2002 Sep

25;98(2-3):243-54. Review. PubMed PMID: 12141990.


Mäder U, Homuth G, Scharf C, Büttner K, Bode R, Hecker M. Transcriptome and

proteome analysis of Bacillus subtilis gene expression modulated by amino acid

availability. J Bacteriol. 2002 Aug;184(15):4288-95. PubMed PMID: 12107147;

PubMed Central PMCID: PMC135197.


Antelmann H, Yamamoto H, Sekiguchi J, Hecker M. Stabilization of cell wall

proteins in Bacillus subtilis: a proteomic approach. Proteomics. 2002

May;2(5):591-602. PubMed PMID: 11987133.


Eymann C, Homuth G, Scharf C, Hecker M. Bacillus subtilis functional

genomics: global characterization of the stringent response by proteome and

transcriptome analysis. J Bacteriol. 2002 May;184(9):2500-20. PubMed PMID:

11948165; PubMed Central PMCID: PMC134987.


Dobrindt U, Piechaczek K, Schierhorn A, Fischer G, Hecker M, Hacker J.

Influence of the leuX-encoded tRNA5(Leu) on the regulation of gene expression in

pathogenic Escherichia coli. J Mol Microbiol Biotechnol. 2002 May;4(3):205-9.

Review. PubMed PMID: 11931548.


Rosen R, Biran D, Gur E, Becher D, Hecker M, Ron EZ. Protein aggregation in

Escherichia coli: role of proteases. FEMS Microbiol Lett. 2002 Jan

22;207(1):9-12. PubMed PMID: 11886743.


Rosen R, Büttner K, Becher D, Nakahigashi K, Yura T, Hecker M, Ron EZ. Heat

shock proteome of Agrobacterium tumefaciens: evidence for new control systems. J

Bacteriol. 2002 Mar;184(6):1772-8. PubMed PMID: 11872730; PubMed Central PMCID:



Pummi T, Leskelä S, Wahlström E, Gerth U, Tjalsma H, Hecker M, Sarvas M,

Kontinen VP. ClpXP protease regulates the signal peptide cleavage of secretory

preproteins in Bacillus subtilis with a mechanism distinct from that of the Ecs

ABC transporter. J Bacteriol. 2002 Feb;184(4):1010-8. PubMed PMID: 11807061;

PubMed Central PMCID: PMC134792.


Bandow JE, Brötz H, Hecker M. Bacillus subtilis tolerance of moderate

concentrations of rifampin involves the sigma(B)-dependent general and multiple

stress response. J Bacteriol. 2002 Jan;184(2):459-67. PubMed PMID: 11751823;

PubMed Central PMCID: PMC139561.


Ziebandt AK, Weber H, Rudolph J, Schmid R, Höper D, Engelmann S, Hecker M.

Extracellular proteins of Staphylococcus aureus and the role of SarA and sigma B.

Proteomics. 2001 Apr;1(4):480-93. PubMed PMID: 11681202.


Büttner K, Bernhardt J, Scharf C, Schmid R, Mäder U, Eymann C, Antelmann H,

Völker A, Völker U, Hecker M. A comprehensive two-dimensional map of cytosolic

proteins of Bacillus subtilis. Electrophoresis. 2001 Aug;22(14):2908-35. PubMed

PMID: 11565787.


Petersohn A, Brigulla M, Haas S, Hoheisel JD, Völker U, Hecker M. Global

analysis of the general stress response of Bacillus subtilis. J Bacteriol. 2001

Oct;183(19):5617-31. PubMed PMID: 11544224; PubMed Central PMCID: PMC95453.


Antelmann H, Tjalsma H, Voigt B, Ohlmeier S, Bron S, van Dijl JM, Hecker M.

A proteomic view on genome-based signal peptide predictions. Genome Res. 2001

Sep;11(9):1484-502. PubMed PMID: 11544192.


Ludwig H, Homuth G, Schmalisch M, Dyka FM, Hecker M, Stülke J. Transcription

of glycolytic genes and operons in Bacillus subtilis: evidence for the presence

of multiple levels of control of the gapA operon. Mol Microbiol. 2001

Jul;41(2):409-22. PubMed PMID: 11489127.


Hecker M, Völker U. General stress response of Bacillus subtilis and other

bacteria. Adv Microb Physiol. 2001;44:35-91. Review. PubMed PMID: 11407115.


Eymann C, Hecker M. Induction of sigma(B)-dependent general stress genes by

amino acid starvation in a spo0H mutant of Bacillus subtilis. FEMS Microbiol

Lett. 2001 May 30;199(2):221-7. PubMed PMID: 11377871.


Jürgen B, Hanschke R, Sarvas M, Hecker M, Schweder T. Proteome and

transcriptome based analysis of Bacillus subtilis cells overproducing an

insoluble heterologous protein. Appl Microbiol Biotechnol. 2001 Apr;55(3):326-32.

PubMed PMID: 11341315.


Rosen R, Büttner K, Schmid R, Hecker M, Ron EZ. Stress-induced proteins of

Agrobacterium tumefaciens. FEMS Microbiol Ecol. 2001 May;35(3):277-285. PubMed

PMID: 11311438.


Ohlmeier S, Scharf C, Hecker M. Alkaline proteins of Bacillus subtilis:

first steps towards a two-dimensional alkaline master gel. Electrophoresis. 2000

Nov;21(17):3701-9. PubMed PMID: 11271489.


Eymann C, Mittenhuber G, Hecker M. The stringent response, sigmaH-dependent

gene expression and sporulation in Bacillus subtilis. Mol Gen Genet. 2001

Feb;264(6):913-23. PubMed PMID: 11254139.


Krüger E, Zühlke D, Witt E, Ludwig H, Hecker M. Clp-mediated proteolysis in

Gram-positive bacteria is autoregulated by the stability of a repressor. EMBO J.

2001 Feb 15;20(4):852-63. PubMed PMID: 11179229; PubMed Central PMCID: PMC145420.


Enfors SO, Jahic M, Rozkov A, Xu B, Hecker M, Jürgen B, Krüger E, Schweder

T, Hamer G, O’Beirne D, Noisommit-Rizzi N, Reuss M, Boone L, Hewitt C, McFarlane

C, Nienow A, Kovacs T, Trägårdh C, Fuchs L, Revstedt J, Friberg PC, Hjertager B,

Blomsten G, Skogman H, Hjort S, Hoeks F, Lin HY, Neubauer P, van der Lans R,

Luyben K, Vrabel P, Manelius A. Physiological responses to mixing in large scale

bioreactors. J Biotechnol. 2001 Feb 13;85(2):175-85. PubMed PMID: 11165362.


Zuber U, Drzewiecki K, Hecker M. Putative sigma factor SigI (YkoZ) of

Bacillus subtilis is induced by heat shock. J Bacteriol. 2001 Feb;183(4):1472-5.

PubMed PMID: 11157964; PubMed Central PMCID: PMC95025.


Dobrindt U, Janke B, Piechaczek K, Nagy G, Ziebuhr W, Fischer G, Schierhorn

A, Hecker M, Blum-Oehler G, Hacker J. Toxin genes on pathogenicity islands:

impact for microbial evolution. Int J Med Microbiol. 2000 Oct;290(4-5):307-11.

Review. PubMed PMID: 11111903.


Gertz S, Engelmann S, Schmid R, Ziebandt AK, Tischer K, Scharf C, Hacker J,

Hecker M. Characterization of the sigma(B) regulon in Staphylococcus aureus. J

Bacteriol. 2000 Dec;182(24):6983-91. PubMed PMID: 11092859; PubMed Central PMCID:



Rachid S, Ohlsen K, Wallner U, Hacker J, Hecker M, Ziebuhr W. Alternative

transcription factor sigma(B) is involved in regulation of biofilm expression in

a Staphylococcus aureus mucosal isolate. J Bacteriol. 2000 Dec;182(23):6824-6.

PubMed PMID: 11073930; PubMed Central PMCID: PMC111428.


Hecker M, Engelmann S. Proteomics, DNA arrays and the analysis of still

unknown regulons and unknown proteins of Bacillus subtilis and pathogenic

gram-positive bacteria. Int J Med Microbiol. 2000 May;290(2):123-34. Review.

PubMed PMID: 11045917.


Piechaczek K, Dobrindt U, Schierhorn A, Fischer GS, Hecker M, Hacker J.

Influence of pathogenicity islands and the minor leuX-encoded tRNA5Leu on the

proteome pattern of the uropathogenic Escherichia coli strain 536. Int J Med

Microbiol. 2000 Mar;290(1):75-84. PubMed PMID: 11043984.


Lorenz U, Ohlsen K, Karch H, Hecker M, Thiede A, Hacker J. Human antibody

response during sepsis against targets expressed by methicillin resistant

Staphylococcus aureus. FEMS Immunol Med Microbiol. 2000 Oct;29(2):145-53. PubMed

PMID: 11024354.


Jongbloed JD, Martin U, Antelmann H, Hecker M, Tjalsma H, Venema G, Bron S,

van Dijl JM, Müller J. TatC is a specificity determinant for protein secretion

via the twin-arginine translocation pathway. J Biol Chem. 2000 Dec

29;275(52):41350-7. PubMed PMID: 11007775.


Jürgen B, Lin HY, Riemschneider S, Scharf C, Neubauer P, Schmid R, Hecker M,

Schweder T. Monitoring of genes that respond to overproduction of an insoluble

recombinant protein in Escherichia coli glucose-limited fed-batch fermentations.

Biotechnol Bioeng. 2000 Oct 20;70(2):217-24. PubMed PMID: 10972933.


Faires N, Tobisch S, Bachem S, Martin-Verstraete I, Hecker M, Stülke J. The

catabolite control protein CcpA controls ammonium assimilation in Bacillus

subtilis. J Mol Microbiol Biotechnol. 1999 Aug;1(1):141-8. PubMed PMID: 10941796.


Antelmann H, Scharf C, Hecker M. Phosphate starvation-inducible proteins of

Bacillus subtilis: proteomics and transcriptional analysis. J Bacteriol. 2000

Aug;182(16):4478-90. PubMed PMID: 10913081; PubMed Central PMCID: PMC94619.


Krüger E, Witt E, Ohlmeier S, Hanschke R, Hecker M. The clp proteases of

Bacillus subtilis are directly involved in degradation of misfolded proteins. J

Bacteriol. 2000 Jun;182(11):3259-65. PubMed PMID: 10809708; PubMed Central PMCID:


before 2000

Tobisch S, Zühlke D, Bernhardt J, Stülke J, Hecker M. Role of CcpA in

regulation of the central pathways of carbon catabolism in Bacillus subtilis. J

Bacteriol. 1999 Nov;181(22):6996-7004. PubMed PMID: 10559165; PubMed Central

PMCID: PMC94174.


Petersohn A, Engelmann S, Setlow P, Hecker M. The katX gene of Bacillus

subtilis is under dual control of sigmaB and sigmaF. Mol Gen Genet. 1999

Aug;262(1):173-9. PubMed PMID: 10503549.


Bernhardt J, Büttner K, Scharf C, Hecker M. Dual channel imaging of

two-dimensional electropherograms in Bacillus subtilis. Electrophoresis. 1999

Aug;20(11):2225-40. PubMed PMID: 10493127.


Petersohn A, Bernhardt J, Gerth U, Höper D, Koburger T, Völker U, Hecker M.

Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter

consensus-directed search and oligonucleotide hybridization. J Bacteriol. 1999

Sep;181(18):5718-24. PubMed PMID: 10482513; PubMed Central PMCID: PMC94092.


Schweder T, Krüger E, Xu B, Jürgen B, Blomsten G, Enfors SO, Hecker M.

Monitoring of genes that respond to process-related stress in large-scale

bioprocesses. Biotechnol Bioeng. 1999 Oct 20;65(2):151-9. PubMed PMID: 10458735.


Tobisch S, Stülke J, Hecker M. Regulation of the lic operon of Bacillus

subtilis and characterization of potential phosphorylation sites of the LicR

regulator protein by site-directed mutagenesis. J Bacteriol. 1999

Aug;181(16):4995-5003. PubMed PMID: 10438772; PubMed Central PMCID: PMC93989.


Völker U, Maul B, Hecker M. Expression of the sigmaB-dependent general

stress regulon confers multiple stress resistance in Bacillus subtilis. J

Bacteriol. 1999 Jul;181(13):3942-8. PubMed PMID: 10383961; PubMed Central PMCID:



Schweder T, Kolyschkow A, Völker U, Hecker M. Analysis of the expression and

function of the sigmaB-dependent general stress regulon of Bacillus subtilis

during slow growth. Arch Microbiol. 1999 May-Jun;171(6):439-43. PubMed PMID:



Gertz S, Engelmann S, Schmid R, Ohlsen K, Hacker J, Hecker M. Regulation of

sigmaB-dependent transcription of sigB and asp23 in two different Staphylococcus

aureus strains. Mol Gen Genet. 1999 Apr;261(3):558-66. PubMed PMID: 10323238.


Petersohn A, Antelmann H, Gerth U, Hecker M. Identification and

transcriptional analysis of new members of the sigmaB regulon in Bacillus

subtilis. Microbiology. 1999 Apr;145 ( Pt 4):869-80. PubMed PMID: 10220166.


Lindner C, Galinier A, Hecker M, Deutscher J. Regulation of the activity of

the Bacillus subtilis antiterminator LicT by multiple PEP-dependent, enzyme I-

and HPr-catalysed phosphorylation. Mol Microbiol. 1999 Feb;31(3):995-1006. PubMed

PMID: 10048041.


Krüger E, Hecker M. The first gene of the Bacillus subtilis clpC operon,

ctsR, encodes a negative regulator of its own operon and other class III heat

shock genes. J Bacteriol. 1998 Dec;180(24):6681-8. PubMed PMID: 9852015; PubMed

Central PMCID: PMC107774.


Drzewiecki K, Eymann C, Mittenhuber G, Hecker M. The yvyD gene of Bacillus

subtilis is under dual control of sigmaB and sigmaH. J Bacteriol. 1998

Dec;180(24):6674-80. PubMed PMID: 9852014; PubMed Central PMCID: PMC107773.


Hecker M, Völker U. Non-specific, general and multiple stress resistance of

growth-restricted Bacillus subtilis cells by the expression of the sigmaB

regulon. Mol Microbiol. 1998 Sep;29(5):1129-36. Review. PubMed PMID: 9767581.


Völker U, Andersen KK, Antelmann H, Devine KM, Hecker M. One of two osmC

homologs in Bacillus subtilis is part of the sigmaB-dependent general stress

regulon. J Bacteriol. 1998 Aug;180(16):4212-8. PubMed PMID: 9696771; PubMed

Central PMCID: PMC107419.


Jürgen B, Schweder T, Hecker M. The stability of mRNA from the gsiB gene of

Bacillus subtilis is dependent on the presence of a strong ribosome binding site.

Mol Gen Genet. 1998 Jun;258(5):538-45. PubMed PMID: 9669336.


Gerth U, Krüger E, Derré I, Msadek T, Hecker M. Stress induction of the

Bacillus subtilis clpP gene encoding a homologue of the proteolytic component of

the Clp protease and the involvement of ClpP and ClpX in stress tolerance. Mol

Microbiol. 1998 May;28(4):787-802. PubMed PMID: 9643546.


Mogk A, Völker A, Engelmann S, Hecker M, Schumann W, Völker U. Nonnative

proteins induce expression of the Bacillus subtilis CIRCE regulon. J Bacteriol.

1998 Jun;180(11):2895-900. PubMed PMID: 9603878; PubMed Central PMCID: PMC107255.


Scharf C, Riethdorf S, Ernst H, Engelmann S, Völker U, Hecker M. Thioredoxin

is an essential protein induced by multiple stresses in Bacillus subtilis. J

Bacteriol. 1998 Apr;180(7):1869-77. PubMed PMID: 9537387; PubMed Central PMCID:


Antelmann H, Engelmann S, Schmid R, Sorokin A, Lapidus A, Hecker M.

Expression of a stress- and starvation-induced dps/pexB-homologous gene is

controlled by the alternative sigma factor sigmaB in Bacillus subtilis. J

Bacteriol. 1997 Dec;179(23):7251-6. PubMed PMID: 9393687; PubMed Central PMCID:



Antelmann H, Schmid R, Hecker M. The NAD synthetase NadE (OutB) of Bacillus

subtilis is a sigma B-dependent general stress protein. FEMS Microbiol Lett. 1997

Aug 15;153(2):405-9. PubMed PMID: 9271869.


Antelmann H, Bernhardt J, Schmid R, Mach H, Völker U, Hecker M. First steps

from a two-dimensional protein index towards a response-regulation map for

Bacillus subtilis. Electrophoresis. 1997 Aug;18(8):1451-63. PubMed PMID: 9298659.


Krüger E, Msadek T, Ohlmeier S, Hecker M. The Bacillus subtilis clpC operon

encodes DNA repair and competence proteins. Microbiology. 1997 Apr;143 ( Pt

4):1309-16. PubMed PMID: 9141693.


Bernhardt J, Völker U, Völker A, Antelmann H, Schmid R, Mach H, Hecker M.

Specific and general stress proteins in Bacillus subtilis–a two-deimensional

protein electrophoresis study. Microbiology. 1997 Mar;143(Pt 3):999-1017. PubMed

PMID: 9296790.


Schmid R, Bernhardt J, Antelmann H, Völker A, Mach H, Völker U, Hecker M.

Identification of vegetative proteins for a two-dimensional protein index of

Bacillus subtilis. Microbiology. 1997 Mar;143 ( Pt 3):991-8. PubMed PMID:



Büttner K, Pich A, Neubauer P, Schmid R, Bahl H, Hecker M. Copurification of

ribosomal protein S2 and DNA-dependent RNA polymerase from heat-shocked cells of

Bacillus subtilis. J Basic Microbiol. 1997;37(1):3-9. PubMed PMID: 9090122.


Tobisch S, Glaser P, Krüger S, Hecker M. Identification and characterization

of a new beta-glucoside utilization system in Bacillus subtilis. J Bacteriol.

1997 Jan;179(2):496-506. PubMed PMID: 8990303; PubMed Central PMCID: PMC178721.


Gerth U, Wipat A, Harwood CR, Carter N, Emmerson PT, Hecker M. Sequence and

transcriptional analysis of clpX, a class-III heat-shock gene of Bacillus

subtilis. Gene. 1996 Nov 28;181(1-2):77-83. PubMed PMID: 8973311.


Engelmann S, Hecker M. Impaired oxidative stress resistance of Bacillus

subtilis sigB mutants and the role of katA and katE. FEMS Microbiol Lett. 1996

Nov 15;145(1):63-9. PubMed PMID: 8931328.


Eymann C, Mach H, Harwood CR, Hecker M. Phosphate-starvation-inducible

proteins in Bacillus subtilis: a two-dimensional gel electrophoresis study.

Microbiology. 1996 Nov;142 ( Pt 11):3163-70. PubMed PMID: 8969513.


Antelmann H, Engelmann S, Schmid R, Hecker M. General and oxidative stress

responses in Bacillus subtilis: cloning, expression, and mutation of the alkyl

hydroperoxide reductase operon. J Bacteriol. 1996 Nov;178(22):6571-8. PubMed

PMID: 8932314; PubMed Central PMCID: PMC178544.


Krüger E, Msadek T, Hecker M. Alternate promoters direct stress-induced

transcription of the Bacillus subtilis clpC operon. Mol Microbiol. 1996

May;20(4):713-23. PubMed PMID: 8793870.


Krüger S, Gertz S, Hecker M. Transcriptional analysis of bglPH expression in

Bacillus subtilis: evidence for two distinct pathways mediating carbon catabolite

repression. J Bacteriol. 1996 May;178(9):2637-44. PubMed PMID: 8626332; PubMed

Central PMCID: PMC177989.


Schnetz K, Stülke J, Gertz S, Krüger S, Krieg M, Hecker M, Rak B. LicT, a

Bacillus subtilis transcriptional antiterminator protein of the BglG family. J

Bacteriol. 1996 Apr;178(7):1971-9. PubMed PMID: 8606172; PubMed Central PMCID:



Hecker M, Schumann W, Völker U. Heat-shock and general stress response in

Bacillus subtilis. Mol Microbiol. 1996 Feb;19(3):417-28. Review. PubMed PMID:



Engelmann S, Lindner C, Hecker M. Cloning, nucleotide sequence, and

regulation of katE encoding a sigma B-dependent catalase in Bacillus subtilis. J

Bacteriol. 1995 Oct;177(19):5598-605. PubMed PMID: 7559348; PubMed Central PMCID:



Krüger S, Hecker M. Regulation of the putative bglPH operon for

aryl-beta-glucoside utilization in Bacillus subtilis. J Bacteriol. 1995

Oct;177(19):5590-7. PubMed PMID: 7559347; PubMed Central PMCID: PMC177369.


Maul B, Völker U, Riethdorf S, Engelmann S, Hecker M. sigma B-dependent

regulation of gsiB in response to multiple stimuli in Bacillus subtilis. Mol Gen

Genet. 1995 Jul 22;248(1):114-20. PubMed PMID: 7651322.


Voelker U, Voelker A, Maul B, Hecker M, Dufour A, Haldenwang WG. Separate

mechanisms activate sigma B of Bacillus subtilis in response to environmental and

metabolic stresses. J Bacteriol. 1995 Jul;177(13):3771-80. PubMed PMID: 7601843;

PubMed Central PMCID: PMC177095.


Antelmann H, Bernhardt J, Schmid R, Hecker M. A gene at 333 degrees on the

Bacillus subtilis chromosome encodes the newly identified sigma B-dependent

general stress protein GspA. J Bacteriol. 1995 Jun;177(12):3540-5. PubMed PMID:

7768864; PubMed Central PMCID: PMC177060.


Schweder T, Hofmann K, Hecker M. Escherichia coli K12 relA strains as safe

hosts for expression of recombinant DNA. Appl Microbiol Biotechnol. 1995

Jan;42(5):718-23. PubMed PMID: 7765912.


Riethdorf S, Völker U, Gerth U, Winkler A, Engelmann S, Hecker M. Cloning,

nucleotide sequence, and expression of the Bacillus subtilis lon gene. J

Bacteriol. 1994 Nov;176(21):6518-27. PubMed PMID: 7961402; PubMed Central PMCID:



Krüger E, Völker U, Hecker M. Stress induction of clpC in Bacillus subtilis

and its involvement in stress tolerance. J Bacteriol. 1994 Jun;176(11):3360-7.

PubMed PMID: 8195092; PubMed Central PMCID: PMC205508.


Lindner C, Stülke J, Hecker M. Regulation of xylanolytic enzymes in Bacillus

subtilis. Microbiology. 1994 Apr;140 ( Pt 4):753-7. PubMed PMID: 8012596.


Völker U, Engelmann S, Maul B, Riethdorf S, Völker A, Schmid R, Mach H,

Hecker M. Analysis of the induction of general stress proteins of Bacillus

subtilis. Microbiology. 1994 Apr;140 ( Pt 4):741-52. PubMed PMID: 8012595.


Krüger S, Stülke J, Hecker M. Catabolite repression of beta-glucanase

synthesis in Bacillus subtilis. J Gen Microbiol. 1993 Sep;139(9):2047-54. PubMed

PMID: 8245831.


Stülke J, Hanschke R, Hecker M. Temporal activation of beta-glucanase

synthesis in Bacillus subtilis is mediated by the GTP pool. J Gen Microbiol. 1993

Sep;139(9):2041-5. PubMed PMID: 8245830.


Völker U, Riethdorf S, Winkler A, Weigend B, Fortnagel P, Hecker M. Cloning

and characterization of heat-inducible promoters of Bacillus subtilis. FEMS

Microbiol Lett. 1993 Feb 1;106(3):287-93. PubMed PMID: 8454194.


Schweder T, Schmidt I, Herrmann H, Neubauer P, Hecker M, Hofmann K. An

expression vector system providing plasmid stability and conditional suicide of

plasmid-containing cells. Appl Microbiol Biotechnol. 1992 Oct;38(1):91-3. PubMed

PMID: 1369014.


Völker U, Mach H, Schmid R, Hecker M. Stress proteins and cross-protection

by heat shock and salt stress in Bacillus subtilis. J Gen Microbiol. 1992

Oct;138(10):2125-35. PubMed PMID: 1362210.


Schmidt A, Schiesswohl M, Völker U, Hecker M, Schumann W. Cloning,

sequencing, mapping, and transcriptional analysis of the groESL operon from

Bacillus subtilis. J Bacteriol. 1992 Jun;174(12):3993-9. PubMed PMID: 1350777;

PubMed Central PMCID: PMC206108.


Wetzstein M, Völker U, Dedio J, Löbau S, Zuber U, Schiesswohl M, Herget C,

Hecker M, Schumann W. Cloning, sequencing, and molecular analysis of the dnaK

locus from Bacillus subtilis. J Bacteriol. 1992 May;174(10):3300-10. PubMed PMID:

1339421; PubMed Central PMCID: PMC205999.


Riethdorf S, Ulrich A, Hecker M. [Replication of ColE 1-related plasmids at

increased growth temperature depending on rom function]. Z Naturforsch C. 1991

Nov-Dec;46(11-12):1063-6. German. PubMed PMID: 1817515.


Wegrzyn G, Neubauer P, Krueger S, Hecker M, Taylor K. Stringent control of

replication of plasmids derived from coliphage lambda. Mol Gen Genet. 1991

Jan;225(1):94-8. PubMed PMID: 1825694.


Riethdorf S, Ulrich A, Völker U, Hecker M. Excretion into the culture medium

of a Bacillus beta-glucanase after overproduction in Escherichia coli. Z

Naturforsch C. 1990 Mar-Apr;45(3-4):240-4. PubMed PMID: 2114118.


Hofmann KH, Neubauer P, Riethdorf S, Hecker M. Amplification of pBR322

plasmid DNA in Escherichia coli relA strains during batch and fed-batch

fermentation. J Basic Microbiol. 1990;30(1):37-41. PubMed PMID: 2187073.


Riethdorf S, Schroeter A, Hecker M. RelA mutation and pBR322 plasmid

amplification in amino acid-starved cells of Escherichia coli. Genet Res. 1989

Dec;54(3):167-71. PubMed PMID: 2695389.


Mach H, Hecker M, Hill I, Schroeter A, Mach F. [Physiologic significance of

“stringent control” in Escherichia coli under extreme starvation]. Z Naturforsch

  1. 1989 Sep-Oct;44(9-10):838-44. German. PubMed PMID: 2686672.


Hecker M, Riethdorf S, Bauer C, Schroeter A, Borriss R. Expression of a

cloned beta-glucanase gene from Bacillus amyloliquefaciens in an Escherichia coli

relA strain after plasmid amplification. Mol Gen Genet. 1988 Dec;215(1):181-3.

PubMed PMID: 3071739.


Hecker M, Heim C, Völker U, Wölfel L. Induction of stress proteins by sodium

chloride treatment in Bacillus subtilis. Arch Microbiol. 1988;150(6):564-6.

PubMed PMID: 3144958.


Schroeter A, Riethdorf S, Hecker M. Amplification of different ColE1

plasmids in an Escherichia coli relA strain. J Basic Microbiol. 1988;28(8):553-5.

PubMed PMID: 3070008.


Riethdorf S, Flunker G, Seidel W, Khairallah S, Berger W, Meixner M, Hecker

M, Mach F, Döhner L. [In vitro translation of mRNA from respiratory syncytial

virus-infected cells and preparation of cDNA]. Arch Exp Veterinarmed. 1987

Sep;41(5):682-5. German. PubMed PMID: 3435222.


Hecker M, Richter A, Schroeter A, Wölfel L, Mach F. [Synthesis of heat shock

proteins following amino acid or oxygen limitation in Bacillus subtilis relA+ and

relA strains]. Z Naturforsch C. 1987 Jul-Aug;42(7-8):941-7. German. PubMed PMID:



Riedel K, Schroeter A, Liebs P, Graba JP, Hecker M, Schrapel D. Formation of

extracellular neutral proteinase and the stringent response in Bacillus subtilis.

Folia Microbiol (Praha). 1987;32(2):96-100. PubMed PMID: 3108120.


Hecker M, Schroeter A, Mach F. Physiological studies on pBR 322 DNA

amplification in an Escherichia coli relA strain. J Basic Microbiol.

1986;26(6):329-33. PubMed PMID: 3543293.


Hecker M, Schroeter A, Träder K, Mach F. Role of relA mutation in the

survival of amino acid-starved Escherichia coli. Arch Microbiol. 1986

Jan;143(4):400-2. PubMed PMID: 3513732.


Hanschke R, Hecker M. Synthesis of poly(A)+-containing RNA during growth in

Escherichia coli relA+ and relA- strains. J Basic Microbiol. 1986;26(6):317-22.

PubMed PMID: 2433424.


Kunze G, Petzoldt C, Bode R, Samsonova I, Hecker M, Birnbaum D.

Transformation of Candida maltosa and Pichia guilliermondii by a plasmid

containing Saccharomyces cerevisiae ARG4 DNA. Curr Genet. 1985;9(3):205-9. PubMed

PMID: 3916721.


Hecker M, Schroeter A. [Synthesis of alkaline phosphatase in a stringent and

a relaxed strain of Escherichia coli under amino acid and phosphate limitation].

J Basic Microbiol. 1985;25(5):341-7. German. PubMed PMID: 3897509.


Wachlin G, Hecker M. [Protein biosynthesis following heat shock in Bacillus

subtilis]. Z Allg Mikrobiol. 1984;24(6):397-401. German. PubMed PMID: 6433588.


Wiehle W, Hecker M, Reichstein B, Mach F. [Formation of plasmid pBR322

oligomers as depending on a tetracycline concentration]. Z Allg Mikrobiol.

1984;24(2):119-24. German. PubMed PMID: 6372270.


Hecker M, Schroeter A. [3H-thymidine incorporation following isoleucine

limitation in stringent and relaxed controlled strains of Escherichia coli]. Z

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Hecker M. [Molecular biology of the germination of Bacillus spores]. Z Allg

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