Dr. Sandra Maaß


Department for Microbial Proteomics

Felix-Hausdorff-Straße 8 - 1. floor - room: 1.57
17489 Greifswald

phone: +49 (0)3834 420 5921
fax:      +49 (0)3834 420 5902
email:   sandra.maass[at]uni-greifswald.de

Curriculum Vitae
since 11/2016 senior scientist with Dörte Becher, University Greifswald
05/2014 – 11/2016 postdoc in the group of Dörte Becher, University Greifswald
09/2007 – 05/2014 PhD-student in the group of Dörte Becher, University Greifswald dissertation on „Relative and absolute protein quantification in bacteria” with Dörte Becher, University of Greifswald
10/2002 – 09/2007 study of Biology, University of Greifswald diploma thesis: “Clp-dependent proteolysis in stationary cells of Bacillus subtils” with Michael Hecker, University of Greifswald
Research Interests
  •  Label free and label based proteomics

  • Absolute protein quantification using targeted as well as global methods

  • Post-translational modifications (especially phosphorylations and ubiquitinations)

  •  Physiological responses of bacteria to infection-related stress conditions 


Chi BK, Huyen NTT, Loi VV, Gruhlke MCH, Schaffer M, Mäder U, Maaß S, Becher D, Bernhardt J, Arbach M, Hamilton CJ, Slusarenka AJ, Antelmann H (2019). The disulfide stress response and protein S-thioallylation caused by allicin and diallyl polysulfanes in Bacillus subtilis as revealed by transcriptomics and proteomics. Antioxidants 8, 605. PMID: 31795512

Antelo-Varela M, Bartel J, Quesada-Ganuza A, Appel K, Bernal-Cabas M, Sura T, Otto A, Rasmussen M, van Dijl JM, Nielsen A, Maaß S, Becher D (2019). Ariadne’s thread in the analytical labyrinth of membrane proteins: integration of targeted and shotgun proteomics for global absolute quantification of membrane proteins. Anal. Chem. 91 (18), 11972-11980. PMID:31424929

Graf AC, Leonard A, Schäuble M, Rieckmann LM, Hoyer J, Maaß S, Lalk M, Becher D, Pané-Farré J, Riedel K (2019). Virulence factors produced by Staphylococcus aureus biofilms have a moonlighting function contributing to biofilm integrity. Mol. Cell Proteomics, 18 (6), 1036-1053. PMID: 30850421

Maaß S, Moog G, Becher, D (2019). Subcellular protein fractionation in Legionella pneumophila and preparation of the derived sub-proteomes for analysis by mass spectrometry. In: Buchrieser, C and Hilbi, H (eds.). Legionella: Methods and Protocols, Springer, New York, NY, pp 445–464. PMID:30694509

Lemay M-L, Otto A, Maaß S, Plate K, Becher D, Moineau, S (2019). Investigating Lactococcus lactis MG1363 response to phage p2 infection at the proteome level. Mol. Cell Proteomics, 18 (4), 704-714. PMID: 30679258

Maaß S, Otto A, Albrecht D, Riedel K, Trautwein-Schult A, Becher D. Proteomic signatures of Clostridium difficile stressed with metronidazole, vancomycin, or fidaxomicin. Cells. 2018. 7, 213. PMID:30445773

Trautwein-Schult A, Maaß S, Plate K, Otto A, Becher D. A metabolic labeling strategy for relative protein quantification in Clostridioides difficile. Front. Microbiol. 2018. 9. PMID: 30386308

Bonn F, Maaß S. van Dijl JM. Enrichment of cell surface-associated proteins in gram-positive bacteria by biotinylation or trypsin shaving for mass spectrometry analysis. Methods in Molecular Biology (Clifton, N.J.). 2018. 1841, pp 35–43. PMID:30259478

Junker S, Maaß S, Otto, A, Hecker M, Becher D. Toward the quantitative characterization of arginine phosphorylations in Staphylococcus aureus. J. Proteome Res. 2018. doi:10.1021/acs.jproteome.8b0057911. PMID:30358407

Maaß S. Absolute protein quantification using AQUA-calibrated 2D-PAGE. Methods in Molecular Biology (Clifton, N.J.). 2018. 1841, pp 141–16212. PMID:30259485

Otto A, Maaß S, Bonn F, Büttner K, Becher D. An easy and fast protocol for affinity bead-based protein enrichment and storage of proteome samples. In: Shukla, AK (ed.). Methods in Enzymology. 2017. 585, Academic Press, pp 1–13.

Hoyer J, Bartel J, Gómez-Mejia A, Rohde M, Hirschfeld C, Heß N, Sura T, Maaß S, Hammerschmidt S, Becher D. Proteomic response of Streptococcus pneumoniae to iron limitation. International Journal of Medical Microbiology. 2018. doi:10.1016/j.ijmm.2018.02.001. PMID:29496408

Junker S, Maaß S, Otto A, Michalik S, Morgenroth F, Gerth U, et al.. Spectral library based analysis of arginine phosphorylations in Staphylococcus aureus. Mol. Cell Proteomics. 2017.doi:10.1074/mcp.RA117.000378. PMID:29183913

Hillion M, Imber M, Pedre B, Bernhardt J, Saleh M, Loi VV, et al. The glyceraldehyde-3-phosphate dehydrogenase GapDH of Corynebacterium diphtheriae is redox-controlled by protein S -mycothiolation under oxidative stress. Scientific Reports. 2017. 7, 5020. PMID:28694441

Hillion M, Bernhardt J, Busche T, Rossius M, Maaß S, Becher D, et al. Monitoring global protein thiol-oxidation and protein S-mycothiolation in Mycobacterium smegmatis under hypochlorite stress. Sci Rep. 2017. 7, 1195. PMID:28446771

Bonn F, Maass S, Becher D. Sample preparation for mass-spectrometry based absolute protein quantification in antibiotic stress research. In: Sass, P (ed.). Antibiotics, 2017, Springer New York, pp 281–289. PMID:27873259

Otto A, Maaß S, Lassek C, Becher D, Hecker M, Riedel K, Sievers S. The protein inventory of Clostridium difficile grown in complex and minimal medium. Proteomics Clin Appl. 2016. 10(9-10):1068-1072. PMID:27511832

Zühlke D, Dörries K, Bernhardt J, Maaß S, Muntel J, Liebscher V, Pané-Farré J, Riedel K, Lalk M, Völker U, Engelmann S, Becher D, Fuchs S, Hecker M. Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis. Scientific Reports. 2016. 6:28172. PMID: 27344979

Maaß S, Becher D. Methods and applications of absolute protein quantification in microbial systems. J Proteomics. 2016. 136:222–233. PMID:26825536

Salzberg LI, Botella E, Hokamp K, Antelmann H, Maaß S, Becher D, Noone D, Devine KM. A genome-wide analysis of PhoP∼P binding to chromosomal DNA reveals several novel features of the PhoPR-mediated phosphate limitation response in Bacillus subtilis. J. Bacteriol. 2015. 197(8):1492-1506. PMID: 25666134

Kohlstedt M, Sappa PK, Maaß S, Zaprasis A, Hoffmann T, Becker J, Steil L, Hecker M, van Dijl JM, Lalk M, Mäder U, Stülke J, Bremer E, Völker U, Wittmann C. Adaptation of Bacillus subtilis carbon core metabolism to simultaneous nutrient limitation and osmotic challenge: a multi-omics perspective. Environ Microbiol. 2014. 16(6):1898-1917. PMID:24571712

Maaß S, Wachlin G, Bernhardt J, Eymann C, Fromion V, Riedel K, Becher, D, Hecker M. Highly precise quantification of protein molecules per cell during stress and starvation responses in Bacillus subtilis. Mol Cell Proteomics. 2014. 13:2260–2276. PMID: 24878497

Muntel J, Fromion V, Goelzer A, Maaβ S, Mäder U, Büttner K, Hecker M, Becher D. Comprehensive absolute quantification of the cytosolic proteome of Bacillus subtilis by data independent, parallel fragmentation in liquid chromatography/mass spectrometry (LC/MSE). Mol. Cell Proteomics. 2014. 13:1008–1019. PMID: 24696501

Moche M, Albrecht D, Maaß S, Hecker M, Westermeier R, Büttner K. The new horizon in 2D electrophoresis -new technology to increase resolution and sensitivity. Electrophoresis. 2013. 34:1510–1518. PMID: 23494680

Maaß S, Sievers S, Zühlke D, Kuzinski J, Sappa PK, Muntel J, Hessling B, Bernhardt J, Sietmann R, Völker U, Hecker M, Becher D. Efficient, global-scale quantification of absolute protein amounts by integration of targeted mass spectrometry and two-dimensional gel-based proteomics. Analytical Chemistry. 2011. 83:2677–2684. PMID: 21395229

Tefon BE, Maaß S, Ozcengiz E, Becher D, Hecker M, Ozcengiz G. A comprehensive analysis of Bordetella pertussis surface proteome and identification of new immunogenic proteins. Vaccine. 2011. 29:3583–3595. PMID: 21397717