Dr. Annett Mikolasch

Senior Scientist, Group Leader

Department for Bacterial Physiology

Felix-Hausdorff-Straße 8 - 1. floor  - room-nr.: 1.53

17489 Greifswald

phone: +49 (0)3834 420 5917
fax:      +49 (0)3834 420 5902
email:   annett.mikolasch(at)uni-greifswald(dot)de

Curriculum Vitae
2004 – present Assistant for Research and Teaching at the Institute for Microbiology of the Ernst-Moritz-Arndt-University in Greifswald. Studying the degradation/transformation of pollutants by microorganisms and the white biotechnology – laccases as catalysts.

Lectures in Microbial Degradation of Natural Products and Xenobiotics.

Experiences in dealing with HPLC/UV-Vis, GC/MS, LS/MS
2001 – 2004 Laboratory supervisor of the working group Biotransformation of the Ganomycin GmbH in Greifswald

Extending the application fields of bio-combinatoric reactions mediated by laccases
2000 Post-doctoral Researcher at the Institute of Marine Biotechnology Invention of the bio-combinatoric reactions mediated by laccases
1994 – 1999
Research for dissertation on Biotransformation of 1-Acylamino-2-alkylamino-4-phenylimidazoles by Microorganisms and Microbial Enzymes
1994 – 1997 PhD Stipend of the foundation Cusanuswerk
1999 Graduation (Doctor of Natural Sciences, field microbiology) at the Institute of Microbiology of the Ernst-Moritz-Arndt-University in Greifswald
1993 Chemistry degree at the Ernst-Moritz-Arndt-University in Greifswald
Research Interests

Assistant for Research and Teaching in Prof. Dr. Urich’s lab at the Institute for Microbiology of the Ernst-Moritz-Arndt-University in Greifswald. Studying the degradation/transformation of pollutants by microorganisms and the white biotechnology – laccases as catalysts.

Lectures in Microbial Degradation of Natural Products and Xenobiotics

Lectures in Microbiology of drinking water and waste water

Experiences in dealing with HPLC/UV-Vis, GC/MS, LS/MS

 

Publications

 

Wieners M, Reinhard A, Förschler M, Scholler M. The Rare Polypore Antrodiella citrinella and Its Special Phenology in the Black Forest National Park (Germany). J Biodivers Endanger Species. 2016 Oct; 4:168. doi:10.4172/2332-2543.1000168


Mikolasch A, Reinhard A, Alimbetova A, Omirbekova A, Pasler L, Schumann P, Kabisch J, Mukasheva T, Schauer F. From oil spills to barley growth – oil-degrading soil bacteria and their promoting effects. J Basic Microbiol.2016 Sep 14. PMID:27624187.

Zühlke M-K, Schlüter R, Henning A-K, Lipka M, Mikolasch A, Schumann P, Giersberg M, Kunze G, Schauer F. A novel mechanism of conjugate formation of bisphenol A and its analogues by Bacillus amyloliquefaciens: Detoxification and reduction of estrogenicity of bisphenols. Int Biodeterior Biodegrad. 2016 Jan; 109:165-173.

Schlippert M, Mikolasch A, Hahn V, Schauer F. Enzymatic thiol Michael addition using laccases: Multiple C-S bond formation between p-hydroquinones and aromatic thiols. J Mol Catal B-Enzym. 2016 Jan;126: 106–114.

Hahn V, Mikolasch A, Kuhlisch C, Schauer F. Laccase-mediated multi-step homo- and heteromolecular reactions of ortho-dihydroxylated aromatic compounds and mono- or diaminated substances resulting in C-C, C-O and C-N bonds. J Mol Catal B-Enzym. 2015 Aug; 122: 56–63.

Brack C, Mikolasch A, Pukall R, Schumann P, Köster M, Schauer F. Bacteriolytic Bacillus species isolated from brackish waters of the Southern Baltic Sea. Mar Biol. 2013 May; 160:2699–2709 DOI 10.1007/s00227-013-2263-z.

Herter S, Michalik D, Mikolasch A, Schmidt M, Wohlgemuth R, Bornscheuer U, Schauer F. Laccase-mediated synthesis of 2-methoxy-3-methyl-5-(alkylamino)- and 3-methyl-2,5-bis(alkylamino)-[1,4]-benzoquinones. J Mol Catal B-Enzym. 2013 Feb; 90:91– 97.

Hahn V, Mikolasch A, (2011). Mussel glue and its prospects in biotechnology . In Mussels: Anatomy,Habitat and Environmental Impact. McGevin, L.E. (ed.) Nova Science Publishers, ISBN: 978-1-61122-149-7.

Herter S, Mikolasch A, Michalik D, Hammer E, Schauer F, Bornscheuer U, Schmidt M.). C-N coupling of 3-methylcatechol with primary amines using native and recombinant laccases from Trametes versicolor and Pycnoporus cinnabarinus. Tetrahedron. 2011 Oct; 67:9311-9321.

Hahn V, Davids T, Lalk M, Schauer F, Mikolasch A. Enzymatic cyclizations using laccases: Multiple bond formation between dihydroxybenzoic acid derivatives and aromatic amines. Green Chem. 2010 March; 12, 879–887.

Kordon K, Mikolasch A, Schauer F. Oxidative dehalogenation of chlorinated hydroxybiphenyls by laccases of white-rot fungi. Int Biodeterior Biodegrad. 2010 Feb; 64:203-209.

Nhi-Cong LT, Mikolasch A, Klenk HP, Schauer F. Degradation of the multiple branched alkane 2,6,10,14-tetramethyl-pentadecane (pristane) in Rhodococcus ruber and Mycobacterium neoaurum. Int Biodeterior Biodegrad. 2009 Nov; 63:201-207.

Hahn V, Mikolasch A, Manda K, Gördes D, Thurow K, Schauer F. Derivatization of amino acids by fungal laccases: Comparison of enzymatic and chemical methods. J Mol Catal B-Enzym. 2009 April; 60: 76–81.

Awe A, Mikolasch A, Hammer E, Schauer F. Degradation of phenylalkanes and characterization of aromatic intermediates acting as growth inhibiting substances in hydrocarbon utilizing yeast Candida maltose. Int Biodeterior Biodegrad. 2008 Jun; 62: 408–414.

Manda K, Hammer E, Mikolasch A, Niedermeyer T, Dec J, Jones AD, Benesi AJ, Schauer F, Bollag JM. Laccase-induced cross-coupling of 4-aminobenzoic acid with para-dihydroxylated compounds 2,5-dihydroxy-N-(2-hydroxyethyl)-benzamide and 2,5-dihydroxybenzoic acid methyl ester. J Mol Catal B-Enzym. 2005 Jul; 35:86-92.

Mikolasch A, Hammer E, Jonas U, Popowski K, Stielow A Schauer F. Synthesis of 3-(3,4-dihydroxyphenyl)-propionic acid derivatives by N-coupling of amines using laccase. Tetrahedron. 2002; 58:7589-7593.

  • Mikolasch A, Hildebrandt O, Schlüter R, Hammer E, Witt S, Lindequist U. Targeted synthesis of novel β-lactam antibiotics by laccase-catalyzed reaction of aromatic substrates selected by pre-testing for their antimicrobial and cytotoxic activity. Appl Microbiol Biotechnol.2016 Jan 18. PMID: 26780358.

  • Brack C, Mikolasch A, Schlueter R, Otto A, Becher D, Wegner U, Albrecht D, Riedel K, Schauer F. Antibacterial metabolites and bacteriolytic enzymes produced by Bacillus pumilus during bacteriolysis of Arthrobacter citreus. Mar Biotechnol (NY).2015 Jun;17(3):290-304. PMID: 25678259.

  • Mikolasch A, Omirbekova A, Schumann P, Reinhard A, Sheikhany H, Berzhanova R, Mukasheva T, Schauer F. Enrichment of aliphatic, alicyclic and aromatic acids by oil-degrading bacteria isolated from the rhizosphere of plants growing in oil-contaminated soil from Kazakhstan. Appl Microbiol Biotechnol.2015 May;99(9):4071-84. PMID: 25592733.

  • Brack C, Mikolasch A, Schauer F. 2,5-Diketopiperazines produced by Bacillus pumilus during bacteriolysis of Arthrobacter citreus. Mar Biotechnol (NY).2014 Aug;16(4):385-95. PMID: 24449388.

  • Schlueter R, Röder A, Czekalski N, Gliesche D, Mikolasch A, Schauer F. Novel mechanisms of biotransformation of p-tert-amylphenol by bacteria and fungi with special degradation abilities and simultaneous detoxification of the disinfectant. Appl Microbiol Biotechnol.2014 Jan;98(1):373-84. PMID: 24158734.

  • Hahn V, Sünwoldt K, Mikolasch A, Schauer F. Biotransformation of 4-sec-butylphenol by Gram-positive bacteria of the genera Mycobacterium and Nocardia including modifications on the alkyl chain and the hydroxyl group. Appl Microbiol Biotechnol.2013 Sep;97(18):8329-39. PMID: 23912120.

  • Hahn V, Mikolasch A, Schauer F. Cleavage and synthesis function of high and low redox potential laccases towards 4-morpholinoaniline and aminated as well as chlorinated phenols. Appl Microbiol Biotechnol.2014 Feb;98(4):1609-20. PMID: 23715853.

  • Mikolasch A, Manda K, Schlüter R, Lalk M, Witt S, Seefeldt S, Hammer E, Schauer F, Jülich WD, Lindequist U. Comparative analyses of laccase-catalyzed amination reactions for production of novel β-lactam antibiotics. Biotechnol Appl Biochem.2012 Jul-Aug;59(4):295-306. PMID: 23586863.

  • Herter S, Schmidt M, Thompson ML, Mikolasch A, Schauer F. Investigating the effects of metals on phenol oxidase-producing nitrogen-fixing Azotobacter chroococcum. J Basic Microbiol.2013 Jun;53(6):509-17. PMID: 22961388.

  • Hahn V, Sünwoldt K, Mikolasch A, Schauer F. Two different primary oxidation mechanisms during biotransformation of thymol by gram-positive bacteria of the genera Nocardia and Mycobacterium. Appl Microbiol Biotechnol.2013 Feb;97(3):1289-97. PMID: 22828982.

  • Herter S, Schmidt M, Thompson ML, Mikolasch A, Schauer F. Study of enzymatic properties of phenol oxidase from nitrogen-fixing Azotobacter chroococcum. AMB Express.2011 Jun 24;1(1):14. PMID: 21906365.

  • Herter S, Mikolasch A, Schauer F. Identification of phenylalkane derivatives when Mycobacterium neoaurum and Rhodococcus erythropolis were cultured in the presence of various phenylalkanes. Appl Microbiol Biotechnol.2012 Jan;93(1):343-55. PMID: 21701983.

  • Herter S, Schmidt M, Thompson ML, Mikolasch A, Schauer F. A new phenol oxidase produced during melanogenesis and encystment stage in the nitrogen-fixing soil bacterium Azotobacter chroococcum. Appl Microbiol Biotechnol.2011 May;90(3):1037-49. PMID: 21327414.

  • Hahn V, Mikolasch A, Wende K, Bartrow H, Lindequist U, Schauer F. Derivatization of the azole 1-aminobenzotriazole using laccase of Pycnoporus cinnabarinus and Myceliophthora thermophila: influence of methanol on the reaction and biological evaluation of the derivatives. Biotechnol Appl Biochem.2010 May 27;56(2):43-8. PMID: 20441569.

  • Le TN, Mikolasch A, Awe S, Sheikhany H, Klenk HP, Schauer F. Oxidation of aliphatic, branched chain, and aromatic hydrocarbons by Nocardia cyriacigeorgica isolated from oil-polluted sand samples collected in the Saudi Arabian Desert. J Basic Microbiol.2010 Jun;50(3):241-53. PMID: 20143352.

  • Mikolasch A, Hahn V, Manda K, Pump J, Illas N, Gördes D, Lalk M, Gesell Salazar M, Hammer E, Jülich WD, Rawer S, Thurow K, Lindequist U, Schauer F. Laccase-catalyzed cross-linking of amino acids and peptides with dihydroxylated aromatic compounds. Amino Acids.2010 Aug;39(3):671-83. PMID: 20143113.

  • Hahn V, Mikolasch A, Wende K, Bartrow H, Lindequist U, Schauer F. Synthesis of model morpholine derivatives with biological activities by laccase-catalysed reactions. Biotechnol Appl Biochem.2009 Nov 13;54(4):187-95. PMID: 19788410.

  • Awe S, Mikolasch A, Schauer F. Formation of coumarines during the degradation of alkyl substituted aromatic oil components by the yeast Trichosporon asahii. Appl Microbiol Biotechnol.2009 Oct;84(5):965-76. PMID: 19536538.

  • Mikolasch A, Schauer F. Fungal laccases as tools for the synthesis of new hybrid molecules and biomaterials. Appl Microbiol Biotechnol.2009 Mar;82(4):605-24. PMID: 19183983.

  • Waldau D, Mikolasch A, Lalk M, Schauer F. Derivatization of bioactive carbazoles by the biphenyl-degrading bacterium Ralstonia sp. strain SBUG 290. Appl Microbiol Biotechnol.2009 May;83(1):67-75. PMID: 19148631.

  • Waldau D, Methling K, Mikolasch A, Schauer F. Characterization of new oxidation products of 9H-carbazole and structure related compounds by biphenyl-utilizing bacteria. Appl Microbiol Biotechnol.2009 Jan;81(6):1023-31. PMID: 18836714.

  • Hahn V, Mikolasch A, Manda K, Gördes D, Thurow K, Schauer F. Laccase-catalyzed carbon-nitrogen bond formation: coupling and derivatization of unprotected L-phenylalanine with different para-hydroquinones. Amino Acids.2009 Jul;37(2):315-21. PMID: 18695937.

  • Mikolasch A, Matthies A, Lalk M, Schauer F. Laccase-induced C-N coupling of substituted p-hydroquinones with p-aminobenzoic acid in comparison with known chemical routes. Appl Microbiol Biotechnol.2008 Sep;80(3):389-97. PMID: 18668239.

  • Mikolasch A, Wurster M, Lalk M, Witt S, Seefeldt S, Hammer E, Schauer F, Jülich WD, Lindequist U. Novel beta-lactam antibiotics synthesized by amination of catechols using fungal laccase. Chem Pharm Bull (Tokyo).2008 Jul;56(7):902-7. PMID: 18591799.

  • Mikolasch A, Hessel S, Salazar MG, Neumann H, Manda K, Gōrdes D, Schmidt E, Thurow K, Hammer E, Lindequist U, Beller M, Schauer F. Synthesis of new N-analogous corollosporine derivatives with antibacterial activity by laccase-catalyzed amination. Chem Pharm Bull (Tokyo).2008 Jun;56(6):781-6. PMID: 18520080.

  • Manda K, Gördes D, Mikolasch A, Hammer E, Schmidt E, Thurow K, Schauer F. Carbon-oxygen bond formation by fungal laccases: cross-coupling of 2,5-dihydroxy-N-(2-hydroxyethyl)-benzamide with the solvents water, methanol, and other alcohols. Appl Microbiol Biotechnol.2007 Aug;76(2):407-16. PMID: 17576553.

  • Mikolasch A, Niedermeyer TH, Lalk M, Witt S, Seefeldt S, Hammer E, Schauer F, Gesell Salazar M, Hessel S, Jülich WD, Lindequist U. Novel cephalosporins synthesized by amination of 2,5-dihydroxybenzoic acid derivatives using fungal laccases II. Chem Pharm Bull (Tokyo).2007 Mar;55(3):412-6. PMID: 17329882.

  • Poeckel D, Niedermeyer TH, Pham HT, Mikolasch A, Mundt S, Lindequist U, Lalk M, Werz O. Inhibition of human 5-lipoxygenase and anti-neoplastic effects by 2-amino-1,4-benzoquinones. Med Chem.2006 Nov;2(6):591-5. PMID: 17105440.

  • Mikolasch A, Niedermeyer TH, Lalk M, Witt S, Seefeldt S, Hammer E, Schauer F, Gesell M, Hessel S, Jülich WD, Lindequist U. Novel penicillins synthesized by biotransformation using laccase from Trametes spec. Chem Pharm Bull (Tokyo).2006 May;54(5):632-8. PMID: 16651757.

  • Manda K, Hammer E, Mikolasch A, Gördes D, Thurow K, Schauer F. Laccase-induced derivatization of unprotected amino acid L-tryptophan by coupling with p-hydroquinone 2,5-dihydroxy-N-(2-hydroxyethyl)-benzamide. Amino Acids.2006 Nov;31(4):409-19. PMID: 16583315.

  • Niedermeyer TH, Mikolasch A, Lalk M. Nuclear amination catalyzed by fungal laccases: reaction products of p-hydroquinones and primary aromatic amines. J Org Chem.2005 Mar 18;70(6):2002-8. PMID: 15760179.

  • Gesell M, Hammer E, Mikolasch A, Schauer F. Oxidation and ring cleavage of dibenzofuran by the filamentous fungus Paecilomyces lilacinus. Arch Microbiol.2004 Sep;182(1):51-9. PMID: 15278240.

  • Mikolasch A, Schauer F. Biotransformation of N-(2-alkylamino-4-phenylimidazol-1-yl)-acetamides and kinetic studies by using cells and laccase from Trametes versicolor. J Basic Microbiol.2003;43(6):508-21. PMID: 14625901.

  • Mikolasch A, Hammer E, Schauer F. Synthesis of imidazol-2-yl amino acids by using cells from alkane-oxidizing bacteria. Appl Environ Microbiol.2003 Mar;69(3):1670-9. PMID: 12620858.

  • Hammer E, Schoefer L, Schäfer A, Hundt K, Schauer F. Formation of glucoside conjugates during biotransformation of dibenzofuran by Penicillium canescens SBUG-M 1139. Appl Microbiol Biotechnol.2001 Oct;57(3):390-4. PMID: 11759691.

  • Hammer E, Krowas D, Schäfer A, Specht M, Francke W, Schauer F. Isolation and characterization of a dibenzofuran-degrading yeast: identification of oxidation and ring cleavage products. Appl Environ Microbiol.1998 Jun;64(6):2215-9. PMID: 9603837.