Dr. Andreas Otto

Senior Scientist

Department for Microbial Proteomics

Felix-Hausdorff-Straße 8 - 1. floor - room-nr.: 1.57
17489 Greifswald

phone: +49 (0)3834 420 5920
fax:      +49 (0)3834 420 5902
email:   andreas.otto[at]uni-greifswald.de

Curriculum Vitae
since 10/2014 senior scientist with Dörte Becher, University Greifswald
03/2011 – 09/2014 postdoc in the group of Dörte Becher, University Greifswald
10/2004 – 02/2011 dissertation on „Mass spectrometry based proteomics approaches unraveling dynamic processes in the entire bacterial cell ” with Michael Hecker, University of Greifswald
02/2003 – 04/2003 research visit in the group of Michael Yudkin, University of Oxford, UK
10/1999 – 09/2004 study of Biochemistry, University of Greifswald
   
Research Interests
  • Subcellular fractionation of bacterial proteome samples (cytosolic fraction, membrane fraction, surface fraction, extracellular fraction, bacterial vesicles)

  • Systems biology data integration to understand complex physiological processes

  • Label free and label based proteomics

  • Data independent acquisition/ data analysis of LC-MS data

  • Usage of spectral libraries in LC-MS/MS data analysis
Publications
  • Comparative proteome analysis of Actinoplanes sp. SE50/110 grown with maltose or glucose shows minor differences for acarbose biosynthesis proteins but major differences for saccharide transporters. Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Pühler A. J Proteomics. 2016 Jan 10;131:140-8. doi: 10.1016/j.jprot.2015.10.023.

 

  • Global analysis of the impact of linezolid onto virulence factor production in S. aureus USA300. Bonn F, Pané-Farré J, Schlüter R, Schaffer M, Fuchs S, Bernhardt J, Riedel K, Otto A, Völker U, van Dijl JM, Hecker M, Mäder U, Becher D. Int J Med Microbiol. 2016 May;306(3):131-40. doi: 10.1016/j.ijmm.2016.02.004.

 

  • Evolutionary Events Associated with an Outbreak of Meningococcal Disease in Men Who Have Sex with Men. Taha MK, Claus H, Lappann M, Veyrier FJ, Otto A, Becher D, Deghmane AE, Frosch M, Hellenbrand W, Hong E, Parent du Châtelet I, Prior K, Harmsen D, Vogel U. PLoS One. 2016 May 11;11(5):e0154047. doi: 10.1371/journal.pone.0154047.

 

  • Impact of moderate temperature changes on Neisseria meningitidis adhesive phenotypes and proteome. Lappann M, Otto A, Brauer M, Becher D, Vogel U, Johswich K. Infect Immun. 2016 Sep 26. pii: IAI.00584-16.

 

  • The protein inventory of Clostridium difficile grown in complex and minimal medium. Otto A, Maaß S, Lassek C, Becher D, Hecker M, Riedel K, Sievers S. Proteomics Clin Appl. 2016 Oct;10(9-10):1068-1072. doi: 10.1002/prca.201600069.

 

  • Proteomics of septicemic Escherichia coli. Otto A, Biran D, Sura T, Becher D, Ron EZ. Proteomics Clin Appl. 2016 Oct;10(9-10):1020-1024. doi: 10.1002/prca.201600049. Review.

 

  • The Extracellular Proteome and Citrullinome of the Oral Pathogen Porphyromonas gingivalis. Stobernack T, Glasner C, Junker S, Gabarrini G, de Smit M, de Jong A, Otto A, Becher D, van Winkelhoff AJ, van Dijl JM. J Proteome Res. 2016 Oct 7 in press.

 

  • Grad-seq guides the discovery of ProQ as a major small RNA-binding protein. Smirnov A, Förstner KU, Holmqvist E, Otto A, Günster R, Becher D, Reinhardt R, Vogel J. Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):11591-11596.
  • A metaproteomics approach to elucidate host and pathogen protein expression during catheter-associated urinary tract infections (CAUTIs). Lassek C, Burghartz M, Chaves-Moreno D, Otto A, Hentschker C, Fuchs S, Bernhardt J, Jauregui R, Neubauer R, Becher D, Pieper DH, Jahn M, Jahn D, Riedel K. Mol Cell Proteomics. 2015 Apr;14(4):989-1008. doi: 10.1074/mcp.M114.043463.

  • Antibacterial metabolites and bacteriolytic enzymes produced by Bacillus pumilus during bacteriolysis of Arthrobacter citreus. Brack C, Mikolasch A, Schlueter R, Otto A, Becher D, Wegner U, Albrecht D, Riedel K, Schauer F. Mar Biotechnol (NY). 2015 Jun;17(3):290-304. doi: 10.1007/s10126-015-9614-3.

  • Purification and proteomics of pathogen-modified vacuoles and membranes. Herweg JA, Hansmeier N, Otto A, Geffken AC, Subbarayal P, Prusty BK, Becher D, Hensel M, Schaible UE, Rudel T, Hilbi H. Front Cell Infect Microbiol. 2015 Jun 2;5:48. doi: 10.3389/fcimb.2015.00048. Review.

  • Comprehensive proteome analysis of Actinoplanes sp. SE50/110 highlighting the location of proteins encoded by the acarbose and the pyochelin biosynthesis gene cluster. Wendler S, Otto A, Ortseifen V, Bonn F, Neshat A, Schneiker-Bekel S, Walter F, Wolf T, Zemke T, Wehmeier UF, Hecker M, Kalinowski J, Becher D, Pühler A. J Proteomics. 2015 Jul 1;125:1-16. doi: 10.1016/j.jprot.2015.04.013.

  • Enterococcus faecalis Glycolipids Modulate Lipoprotein-Content of the Bacterial Cell Membrane and Host Immune Response. Theilacker C, Diederich AK, Otto A, Sava IG, Wobser D, Bao Y, Hese K, Broszat M, Henneke P, Becher D, Huebner J. PLoS One. 2015 Jul 14;10(7):e0132949. doi: 10.1371/journal.pone.0132949.

  • Versatile vector suite for the extracytoplasmic production and purification of heterologous His-tagged proteins in Lactococcus lactis. Neef J, Milder FJ, Koedijk DG, Klaassens M, Heezius EC, van Strijp JA, Otto A, Becher D, van Dijl JM, Buist G. Appl Microbiol Biotechnol. 2015 Nov;99(21):9037-48. doi: 10.1007/s00253-015-6778-8.

  • Characterization of Helicobacter pylori VacA-containing vacuoles (VCVs), VacA intracellular trafficking and interference with calcium signalling in T lymphocytes. Kern B, Jain U, Utsch C, Otto A, Busch B, Jiménez-Soto L, Becher D, Haas R. Cell Microbiol. 2015 Dec;17(12):1811-32. doi: 10.1111/cmi.12474.

  • Quantitative proteomics in the field of microbiology. Otto A, Becher D, Schmidt F. Proteomics. 2014 Mar;14(4-5):547-65. doi: 10.1002/pmic.201300403. Review.

  • The Staphylococcus aureus proteome. Otto A, van Dijl JM, Hecker M, Becher D. Int J Med Microbiol. 2014 Mar;304(2):110-20. doi: 10.1016/j.ijmm.2013.11.007. Review.

  • The phosphoproteome and its physiological dynamics in Staphylococcus aureus. Bäsell K, Otto A, Junker S, Zühlke D, Rappen GM, Schmidt S, Hentschker C, Macek B, Ohlsen K, Hecker M, Becher D. Int J Med Microbiol. 2014 Mar;304(2):121-32. doi: 10.1016/j.ijmm.2013.11.020.

  • Small cationic antimicrobial peptides delocalize peripheral membrane proteins. Wenzel M, Chiriac AI, Otto A, Zweytick D, May C, Schumacher C, Gust R, Albada HB, Penkova M, Krämer U, Erdmann R, Metzler-Nolte N, Straus SK, Bremer E, Becher D, Brötz-Oesterhelt H, Sahl HG, Bandow JE. Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):E1409-18. doi: 10.1073/pnas.1319900111.

  • Functional characterization of polysaccharide utilization loci in the marine Bacteroidetes 'Gramella forsetii' KT0803. Kabisch A, Otto A, König S, Becher D, Albrecht D, Schüler M, Teeling H, Amann RI, Schweder T. ISME J. 2014 Jul;8(7):1492-502. doi: 10.1038/ismej.2014.4.

  • Functional analysis of novel Rab GTPases identified in the proteome of purified Legionella-containing vacuoles from macrophages. Hoffmann C, Finsel I, Otto A, Pfaffinger G,Rothmeier E, Hecker M, Becher D, Hilbi H. Cell Microbiol. 2014 Jul;16(7):1034-52. doi: 10.1111/cmi.12256.

  • Picking vanished proteins from the void: how to collect and ship/share extremely dilute proteins in a reproducible and highly efficient manner. Bonn F, Bartel J, Büttner K, Hecker M, Otto A, Becher D. Anal Chem. 2014 Aug 5;86(15):7421-7. doi: 10.1021/ac501189j.

  • Coping with anoxia: a comprehensive proteomic and transcriptomic survey of denitrification. Kohlmann Y, Pohlmann A, Schwartz E, Zühlke D, Otto A, Albrecht D, Grimmler C, Ehrenreich A, Voigt B, Becher D, Hecker M, Friedrich B, Cramm R. J Proteome Res. 2014 Oct 3;13(10):4325-38. doi: 10.1021/pr500491r.
  • Novel twin-arginine translocation pathway-dependent phenotypes of Bacillus subtilis unveiled by quantitative proteomics. Goosens VJ, Otto A, Glasner C, Monteferrante CC, van der Ploeg R, Hecker M, Becher D, van Dijl JM. J Proteome Res. 2013 Feb 1;12(2):796-807. doi: 10.1021/pr300866f.

  • Extracytoplasmic proteases determining the cleavage and release of secreted proteins, lipoproteins, and membrane proteins in Bacillus subtilis. Krishnappa L, Dreisbach A, Otto A, Goosens VJ, Cranenburgh RM, Harwood CR, Becher D, van Dijl JM. J Proteome Res. 2013 Sep 6;12(9):4101-10. doi: 10.1021/pr400433h.

  • Comparative proteome analysis of spontaneous outer membrane vesicles and purified outer membranes of Neisseria meningitidis. Lappann M, Otto A, Becher D, Vogel U. J Bacteriol. 2013 Oct;195(19):4425-35. doi: 10.1128/JB.00625-13.

  • Global proteome analysis of vancomycin stress in Staphylococcus aureus. Hessling B, Bonn F, Otto A, Herbst FA, Rappen GM, Bernhardt J, Hecker M, Becher D. Int J Med Microbiol. 2013 Dec;303(8):624-34. doi: 10.1016/j.ijmm.2013.08.014.
  • Substrate-controlled succession of marine bacterioplankton populations induced by a phytoplankton bloom. Teeling H, Fuchs BM, Becher D, Klockow C, Gardebrecht A, Bennke CM, Kassabgy M, Huang S, Mann AJ, Waldmann J, Weber M, Klindworth A, Otto A, Lange J, Bernhardt J, Reinsch C, Hecker M, Peplies J, Bockelmann FD, Callies U, Gerdts G, Wichels A, Wiltshire KH, Glöckner FO, Schweder T, Amann R. Science. 2012 May 4;336(6081):608-11. doi: 10.1126/science.1218344.
  • Global relative and absolute quantitation in microbial proteomics. Otto A, Bernhardt J, Hecker M, Becher D. Curr Opin Microbiol. 2012 Jun;15(3):364-72. doi: 10.1016/j.mib.2012.02.005. Review.
  • Life and death of proteins: a case study of glucose-starved Staphylococcus aureus. Michalik S, Bernhardt J, Otto A, Moche M, Becher D, Meyer H, Lalk M, Schurmann C, Schlüter R, Kock H, Gerth U, Hecker M. Mol Cell Proteomics. 2012 Sep;11(9):558-70. doi: 10.1074/mcp.M112.017004.
  • Bacillus subtilis YqjG is required for genetic competence development. Saller MJ, Otto A, Berrelkamp-Lahpor GA, Becher D, Hecker M, Driessen AJ. Proteomics. 2011 Jan;11(2):270-82. doi: 10.1002/pmic.201000435.

 

  • Analyses of soluble and membrane proteomes of Ralstonia eutropha H16 reveal major changes in the protein complement in adaptation to lithoautotrophy. Kohlmann Y, Pohlmann A, Otto A, Becher D, Cramm R, Lütte S, Schwartz E, Hecker M, Friedrich B. J Proteome Res. 2011 Jun 3;10(6):2767-76. doi: 10.1021/pr101289v.
  • A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study. Albaum SP, Hahne H, Otto A, Haußmann U, Becher D, Poetsch A, Goesmann A, Nattkemper TW. Proteome Sci. 2011 Jun 11;9:30. doi: 10.1186/1477-5956-9-30.

 

  • Surface shaving as a versatile tool to profile global interactions between human serum proteins and the Staphylococcus aureus cell surface. Dreisbach A, van der Kooi-Pol MM, Otto A, Gronau K, Bonarius HP, Westra H, Groen H, Becher D, Hecker M, van Dijl JM. Proteomics. 2011 Jul;11(14):2921-30. doi: 10.1002/pmic.201100134.

 

  • A trigger enzyme in Mycoplasma pneumoniae: impact of the glycerophosphodiesterase GlpQ on virulence and gene expression. Schmidl SR, Otto A, Lluch-Senar M, Piñol J, Busse J, Becher D, Stülke J. PLoS Pathog. 2011 Sep;7(9):e1002263. doi: 10.1371/journal.ppat.1002263.

 

  • Mapping of interactions between human macrophages and Staphylococcus aureus reveals an involvement of MAP kinase signaling in the host defense. Miller M, Dreisbach A, Otto A, Becher D, Bernhardt J, Hecker M, Peppelenbosch MP, van Dijl JM. J Proteome Res. 2011 Sep 2;10(9):4018-32. doi: 10.1021/pr200224x.
  • Systems-wide temporal proteomic profiling in glucose-starved Bacillus subtilis. Otto A, Bernhardt J, Meyer H, Schaffer M, Herbst FA, Siebourg J, Mäder U, Lalk M, Hecker M, Becher D. Nat Commun. 2010;1:137. doi: 10.1038/ncomms1137.
  • A comprehensive proteomics and transcriptomics analysis of Bacillus subtilis salt stress adaptation. Hahne H, Mäder U, Otto A, Bonn F, Steil L, Bremer E, Hecker M, Becher D. J Bacteriol. 2010 Feb;192(3):870-82. doi: 10.1128/JB.01106-09.
  • Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach. Hempel K, Pané-Farré J, Otto A, Sievers S, Hecker M, Becher D. J Proteome Res. 2010 Mar 5;9(3):1579-90. doi: 10.1021/pr901143a.
  • Penicillin-binding protein folding is dependent on the PrsA peptidyl-prolyl cis-trans isomerase in Bacillus subtilis. Hyyryläinen HL, Marciniak BC, Dahncke K, Pietiäinen M, Courtin P, Vitikainen M, Seppala R, Otto A, Becher D, Chapot-Chartier MP, Kuipers OP, Kontinen VP. Mol Microbiol. 2010 Jul 1;77(1):108-27. doi: 10.1111/j.1365-2958.2010.07188.x.
  • Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions. Price CE, Otto A, Fusetti F, Becher D, Hecker M, Driessen AJ. Proteomics. 2010 Sep;10(18):3235-47. doi: 10.1002/pmic.201000284.
  • "Gently rough": the vaccine potential of a Salmonella enterica regulatory lipopolysaccharide mutant. Nagy G, Palkovics T, Otto A, Kusch H, Kocsis B, Dobrindt U, Engelmann S, Hecker M, Emödy L, Pál T, Hacker J. J Infect Dis. 2008 Dec 1;198(11):1699-706. doi: 10.1086/593069.