The Institute of Microbiology hosts a nationwide unique technology platform for microbial proteomics headed by Dörte Becher, Michael Hecker & Katharina Riedel, which offers a broad range of state-of-the-art gel-based and gel-free (meta)proteomics workflows. The platform harbours numerous high-end mass spectrometers together with servers/data analyses pipelines, from which the processed data can be delivered to more sophisticated, often in-house-established bioinformatics analyses and visualization tools. These resources allow a dedicated and individually optimised selection of the best instrument configuration/workflow for each particular research task.

Services

• high resolution and high throughput 2D gel electrophoresis
• in-depth gel image analysis with the Delta2D software package
• high throughput spot cutting and spot digestion
• protein/peptide identification and characterization with mass spectrometry 
• gel-free label-based and label-free proteomics
• data analyses & visualization

Applications

• proteome mapping
• expression profiling
• immunoproteomics
• absolute quantification for systems biology
• identification of post-translational modifications
• protein-protein interactions